油菜素内酯对NaCl胁迫下棉花叶片生理特征和基因表达谱的影响

应用生态学报

油菜素内酯对NaCl胁迫下棉花叶片生理特征和基因表达谱的影响

束红梅,郭书巧,巩元勇,倪万潮^*

(江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室, 南京 210014)

出版日期:2016-01-18 发布日期:2016-01-18

Effects of brassinolide on leaf physiological characteristics and differential gene expression profiles of NaClstressed cotton.

SHU Hong-mei, GUO Shu-qiao, GONG Yuan-yong, NI Wan-chao^*

(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Nanjing 210014, China)

Online:2016-01-18 Published:2016-01-18

摘要/Abstract

摘要： 研究了外源油菜素内酯(BL)对NaCl胁迫下棉花幼苗的钠累积、叶片生理特征及叶片差异基因表达水平的影响.结果表明： NaCl胁迫下2个棉花品种各部位钠含量升高，叶片丙二醛(MDA)、脯氨酸含量上升，叶绿素含量下降，叶片基因表达水平受到影响.外源施用油菜素内酯可降低NaCl胁迫下棉花幼苗根、茎、叶的钠含量，降低叶片中MDA含量，提高脯氨酸含量，并且NaCl胁迫下苏棉12号棉花品种更易受油菜素内酯调控.对苏棉12号的叶片进行数字表达谱分析结果表明，NaCl胁迫下苏棉12号叶片中的差异基因表达水平受到油菜素内酯调控，BL+NaCl处理棉花叶片的基因表达模式与CK(正常生长棉株)更为一致.说明外源油菜素内酯可减轻NaCl胁迫下棉花叶片受抑制程度，从而使NaCl胁迫下棉花叶片生理功能增强(叶绿素含量升高)，最终使生物量增加.

Abstract: This study analyzed the effects of brassinolide (BL) on Na⁺ accumulation, leaf physiological characteristics and differentially expressed genes (DEGs) of cotton leaves under NaCl stress. The results showed that NaCl stress increased the Na⁺, proline and MDA content in the leaves of Sumian 12 and Sumian 22, and changed the expression level of genes in cotton leaves. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Na⁺, enhanced proline content, and resulted in a decrease in the MDA content of NaCl-stressed leaves, and the influence of BL on salt-stressed Sumian 12 plants was more ronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaClstressed cotton leaves, the gene expression pattern as a result of the root applied BL on NaCl-stressed cotton treatment (BL+NaCl) was similar to the normal cotton plants (CK). Our results indicated that brassinolide alleviated NaCl stress on cotton through improving leaf physiological characteristics and gene expression, and resulted in an increase in biomass of NaCl-stressed cotton.

束红梅,郭书巧,巩元勇,倪万潮. 油菜素内酯对NaCl胁迫下棉花叶片生理特征和基因表达谱的影响[J]. 应用生态学报.

SHU Hong-mei, GUO Shu-qiao, GONG Yuan-yong, NI Wan-chao*. Effects of brassinolide on leaf physiological characteristics and differential gene expression profiles of NaClstressed cotton.[J]. Chinese Journal of Applied Ecology.