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灰飞虱对噻嗪酮的抗性风险及机理

毛旭连,刘锦,李许可,迟家家,刘永杰*   

  1. (山东农业大学植物保护学院,  山东泰安 271018)
  • 出版日期:2016-01-18 发布日期:2016-01-18

Resistance risk, cross-resistance and biochemical resistance mechanism of Laodelphax striatellus to buprofezin.

MAO Xu-lian, LIU Jin, LI Xu-ke, CHI Jia-jia, LIU Yong-jie*   

  1. (College of Plant Protection, Shandong Agricultural University, Tai’an 271018, Shandong, China)
  • Online:2016-01-18 Published:2016-01-18

摘要: 为研究灰飞虱对噻嗪酮的抗性发展规律及抗性生化机理,采用稻苗喷雾法对灰飞虱种群进行连续筛选获得高抗性品系,估算其现实遗传力并进行田间抗性风险预测;采用稻苗浸渍法测定杀虫剂对灰飞虱的毒力及交互抗性;利用生物化学方法测定不同品系之间的解毒酶活力,探讨灰飞虱对噻嗪酮的抗性生化机理.结果表明: 用噻嗪酮对灰飞虱种群连续筛选32代,其抗性倍数达到168.49倍,现实遗传力h2为0.11.当杀死率为80%~90%时,预计灰飞虱对噻嗪酮的抗性增长10倍,仅需要5~6代.田间实际的现实遗传力要比室内选择种群估计低一些,预计田间抗性提高10倍所需要时间会更长.交互抗性测定结果表明,灰飞虱抗噻嗪酮品系与吡虫啉和噻虫嗪之间有高水平交互抗性,与啶虫脒有低水平交互抗性,与吡蚜酮和毒死蜱无交互抗性.增效作用和解毒酶活力测定结果显示,抗性品系细胞色素P450单加氧酶活力提高最大,酯酶次之,谷胱甘肽S转移酶无显著变化.田间使用噻嗪酮防治灰飞虱存在较大抗性风险,可与吡蚜酮和毒死蜱等交替使用以延缓抗性发展;3种解毒酶中,细胞色素P450单加氧酶在灰飞虱对噻嗪酮的抗性发展中起到了重要作用.

Abstract: In order to investigate the resistance development law and biochemical resistance mechanism of Laodelphax striatellus to buprofezin, spraying
rice seedlings was used to continuously screen resistant strains of L. striatellus and  dipping rice seedlings was applied to determine the toxicity and crossresistance of L. striatellus to insecticides. After 32generation screening with buprofezin, L. striatellus developed 168.49 folds resistance and its reality heritability (h2) was 0.11. If the killing rate was 80%-90%, L. striatellus was expected to develop 10-fold resistance to buprofezin only after 5 to 6 generations breeding. Because the actual reality heritability of field populations was usually lower than that of the resistant strains, the production of field populations increasing with 10-fold resistance would need much longer time. The results of cross-resistance showed that resistant strain had high level cross-resistance with thiamethoxam and imidacloprid, low level cross-resistance with acetamiprid, and no cross-resistance with pymetrozine and chlorpyrifos. The activity of detoxification enzymes of different strains and the syergism of synergist were measured. The results showed that cytochrome P450 monooxygenase played a major role in the resistance of L. striatellus to buprofezin, the esterase played a minor role and the GSH-S-transferase had no effect. Therefore, L. striatellus would have high risk to develop resistance to buprofezin when used in the field and might be delayed by  using pymetrozine and chlorpyrifos.