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应用生态学报 ›› 2017, Vol. 28 ›› Issue (11): 3740-3750.doi: 10.13287/j.1001-9332.201711.033

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毛竹种植对土壤细菌和真菌群落结构及多样性的影响

赵天心, 毛新伟, 程敏, 陈俊辉, 秦华, 李永春, 梁辰飞, 徐秋芳*   

  1. 浙江农林大学环境与资源学院, 浙江省森林生态系统碳循环与固碳减排重点实验室, 浙江临安 311300
  • 出版日期:2017-11-18 发布日期:2017-11-18
  • 通讯作者: *mail:xuqiufang@zafu.edu.cn
  • 作者简介:赵天心, 男, 1992年生, 硕士研究生.主要从事土壤生物与生物化学研究.E-mail:857936436@qq.com
  • 基金资助:
    本文由国家自然科学基金项目(31570602)和浙江省自然科学基金重点项目(LZ16C160002)资助

Effects of Phyllostachys edulis cultivation on soil bacterial and fungal community structure and diversity

ZHAO Tian-xin, MAO Xin-wei, CHENG Min, CHEN Jun-hui, QIN Hua, LI Yong-chun, LIANG Chen-fei, XU Qiu-fang*   

  1. Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, School of Environment and Resources, Zhejiang Agriculture and Forestry University, Lin’an 311300, Zhejiang, China
  • Online:2017-11-18 Published:2017-11-18
  • Contact: *mail:xuqiufang@zafu.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31570602) and Key Project of Zhejiang Natural Science Foundation, China (LZ16C160002)

摘要: 为揭示天然林改为毛竹林过程中土壤微生物变化规律,在浙江省湖州市安吉县和长兴县两地选择不同种植历史的粗放经营毛竹林,分层采集0~20和20~40 cm的混合土壤样品,应用PCR-DGGE技术分析土壤细菌和真菌群落结构及多样性变化.结果表明: 在马尾松林改种毛竹林或毛竹林入侵杂灌阔叶林形成毛竹纯林过程中,土壤细菌和真菌的群落结构均发生明显变化,且细菌结构对毛竹种植的响应更敏感;随着毛竹生长时间的延长,表层土壤细菌群落表现出抵抗干扰、最后向改种毛竹之前状态恢复的趋势.毛竹种植时间、样地和土层均对土壤细菌和真菌多样性产生显著影响,其中样地和土层的影响明显大于种植时间.土壤性质和细菌、真菌结构的冗余分析结果表明,不同地点、不同土层驱动土壤微生物结构随时间变化的主要因子没有一致规律,且第1、2轴对样地变化的解释率大多低于65.0%,说明除本研究分析的5个土壤化学指标外,可能还有其他土壤理化性质共同驱动微生物结构的变化.

Abstract: This study examined how soil bacterial and fungal communities responded to the cultivation history of Moso bamboo in Anji and Changxing counties, Huzhou, Zhejiang, China. Soil samples (0-20 and 20-40 cm) were taken from bamboo plantations subjected to different cultivation histories and analyzed the community structures of soil bacterial and fungal by PCR-DGGE methods. It was found that soil bacterial and fungal communities varied greatly with the development of bamboo plantations which converted from Masson pine forest or formed via invading adjacent broadleaf shrub forest. Soil bacterial community structures exhibited a greater response to bamboo cultivation time than fungal community, but bacteria structure of surface soil displayed an ability of resiliency to disturbance and the tendency to recover to the original state. The cultivation time, sampling site and soil layer significantly affected the biodiversity of soil bacteria and fungi, especially the latter two factors. Redundancy analysis (RDA) of soil properties and bacteria or fungi communities showed that there were no accordant factors to drive the alteration of microbial structure, and the first two axes explained less than 65.0% of variance for most of the sampling sites and soil layers, indicating there existed soil parameters besides the five examined that contributed to microbial community alteration.