钙与脱落酸对干旱胁迫下黄瓜幼苗光合及相关酶活性的影响

doi:10.13287/j.1001-9332.201612.025

应用生态学报 ›› 2016, Vol. 27 ›› Issue (12): 3996-4002.doi: 10.13287/j.1001-9332.201612.025

钙与脱落酸对干旱胁迫下黄瓜幼苗光合及相关酶活性的影响

陈露露¹, 王秀峰^1,2,3**, 刘美¹, 杨凤娟^1,2, 史庆华^1,2, 魏珉^1,2, 李清明^1,2

¹山东农业大学园艺科学与工程学院, 山东泰安 271018;
²作物生物学国家重点实验室, 山东泰安 271018;
³园艺作物生物学农业部重点开放实验室, 山东泰安 271018

收稿日期:2016-04-12 出版日期:2016-12-18 发布日期:2016-12-18
通讯作者: * E-mail: xfwang@sdau.edu.cn
作者简介:陈露露,女,1989年生,硕士研究生. 主要从事设施蔬菜与无土栽培研究. E-mail: 378018781@qq.com
基金资助:
本文由国家现代农业产业技术体系大宗蔬菜专项(CARS-25)资助

Effects of calcium and ABA on photosynthesis and related enzymes activities in cucumber seedlings under drought stress

CHEN Lu-lu¹, WANG Xiu-feng^1,2,3**, LIU Mei¹, YANG Feng-juan^1,2, SHI Qing-hua^1,2, WEI Min^1,2, LI Qing-ming^1,2

¹College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China;
²State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China;
³Ministry of Agriculture Key Laboratory of Biology of Horticultural Crops, Tai’an 271018, Shandong, China

Received:2016-04-12 Online:2016-12-18 Published:2016-12-18
Contact: * E-mail: xfwang@sdau.edu.cn
Supported by:
This paper was supported by the Special Program of China Modern Agriculture Industry Technology System (CARS-25).

摘要/Abstract

摘要： 为探讨在干旱胁迫下钙与脱落酸对黄瓜幼苗光合作用及相关酶活性的影响,以黄瓜为试材,正常营养液栽培为对照,利用PEG-6000(聚乙二醇)营养液添加模拟干旱胁迫,设干旱胁迫条件下幼苗叶片喷施清水、脱落酸(ABA)、CaCl₂+ABA、LaCl₃(钙离子通道抑制剂)+ABA及EGTA(钙离子螯合剂)+ABA等5个处理.结果表明: 干旱胁迫抑制了黄瓜幼苗的营养生长、降低了幼苗叶片的抗氧化酶和硝酸还原酶活性,以及光合作用和荧光参数等,通过叶面喷施ABA减小了幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,以及光合作用(P_n、g_s)和荧光参数(F_v′/F_m′、q_P和ETR)的下降幅度,有效缓解了干旱胁迫对植株造成的伤害;喷施CaCl₂+ABA显著促进了ABA的这种正向缓解作用,而喷施LaCl₃+ABA和EGTA+ABA都没有表现出促进作用.

Abstract: To investigate the effect of calcium and ABA on photosynthesis and the activities of antioxidant enzymes in cucumber seedlings under drought stress, the cucumber was used as the expe-riment materials, normal nutrient solution culture was considered as the control, and PEG-6000 application in the nutrient solution simulated the drought stress. There were five different treatments which were spraying water, ABA, CaCl₂+ABA, LaCl₃(calcium channel inhibitor)+ABA and EGTA (calcium ion chelating agent)+ABA under drought stress. The results showed that drought stress inhibited the growth of cucumber seedlings, and reduced the activities of antioxidant enzymes, nitrate reductase, net photosynthetic rate and fluorescence parameters of the cucumber seedlings leaves. The application of ABA reduced the inhibition of activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), photosynthesis (P_n, g_s) and the fluorescence parameters (F_v′/F_m′, q_P and ETR), and decreased the damage of drought stress on plant. Spraying CaCl₂+ABAsignificantly promoted the positive effect of ABA, while EGTA+ABA and LaCl₃ +ABA didn’t show the promoting effect.

陈露露, 王秀峰, 刘美, 杨凤娟, 史庆华, 魏珉, 李清明. 钙与脱落酸对干旱胁迫下黄瓜幼苗光合及相关酶活性的影响[J]. 应用生态学报, 2016, 27(12): 3996-4002.

CHEN Lu-lu, WANG Xiu-feng, LIU Mei, YANG Feng-juan, SHI Qing-hua, WEI Min, LI Qing-ming. Effects of calcium and ABA on photosynthesis and related enzymes activities in cucumber seedlings under drought stress[J]. Chinese Journal of Applied Ecology, 2016, 27(12): 3996-4002.

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钙与脱落酸对干旱胁迫下黄瓜幼苗光合及相关酶活性的影响

Effects of calcium and ABA on photosynthesis and related enzymes activities in cucumber seedlings under drought stress

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