靶标昆虫杨扇舟蛾对转Bt基因741杨胁迫效应的SSR分析

doi:10.13287/j.1001-9332.201612.011

应用生态学报 ›› 2016, Vol. 27 ›› Issue (12): 4039-4044.doi: 10.13287/j.1001-9332.201612.011

靶标昆虫杨扇舟蛾对转Bt基因741杨胁迫效应的SSR分析

刘军侠^1,2, 宋晓英¹, 姜文虎¹, 周国娜¹, 高宝嘉^1,2*

¹河北农业大学林学院, 河北保定 071000;
²河北省林木种质资源与森林保护重点实验室, 河北保定 071000

收稿日期:2016-03-24 出版日期:2016-12-18 发布日期:2016-12-18
通讯作者: * E-mail: baojiagao@163.com
作者简介:刘军侠,女,1969年生,博士,教授. 主要从事昆虫生态及有害生物综合治理研究. E-mail: bdljx1997@126.com
基金资助:
本文由国家高技术研究发展计划项目(2013AA102703)和国家林业局科技发展中心项目(JC-2014-04)资助

SSR analysis on stress effect of transgenic hybrid poplar 741 on Clostera anachoreta (Fabricius) (Lepidoptera: Notodontidae)

LIU Jun-xia^1,2, SONG Xiao-ying¹, JIANG Wen-hu¹, ZHOU Guo-na¹, GAO Bao-jia^1,2*

¹College of Forestry, Agricultural University of Hebei, Baoding 071000, Hebei, China;
²Key Laboratory of Germplasm Resources of Forest Trees and Forest Protection, College of Forest of Hebei, Baoding 071000, Hebei, China

Received:2016-03-24 Online:2016-12-18 Published:2016-12-18
Contact: * E-mail: baojiagao@163.com
Supported by:
This work was supported by the National High Technology Research and Development Program of China (2013AA102703) and Science and Technology Development Center of the State Forestry Administration (JC-2014-04).

摘要/Abstract

摘要： 应用SSR分子标记技术,研究了以不同抗性转基因741杨为食物的杨扇舟蛾实验种群的遗传分化,探讨以转Bt基因杨树作为食物对靶标昆虫的胁迫效应.利用筛选出的10对引物,分别对经取食转基因741杨高抗品系‘Pb29’、中抗品系‘Pb17’及未转基因杨(对照)筛选出的杨扇舟蛾实验种群进行遗传多样性和遗传分化研究.结果表明: 10对引物共检测到76条等位基因,平均等位基因7.6个,平均有效等位基因为2.2,平均观测杂合度为0.5167,平均期望杂合度为0.5167,平均多态位点百分率达96.7%.经取食转基因741杨筛选出的杨扇舟蛾实验种群的遗传多样性水平显著高于未转基因种群,且取食转基因741杨的杨扇舟蛾与CK样本间的遗传相似性最低,表明经取食转Bt基因杨树的杨扇舟蛾实验种群遗传多样性有增高趋势.基于SSR分析,转基因741杨对杨扇舟蛾实验种群的胁迫筛选效应明显.

Abstract: The genetic differentiation of the experimental population of Clostera anachoreta fed on different resistant transgenic 741 poplar leaves was analyzed by SSR molecular marker technique to investigate stress effect of transgenic poplar Bt gene as food on target insect. The experimental population of C. anachoreta fed on transgenic 741 poplar high resistant strains ‘Pb29’, medium resis-tant strains ‘Pb17’ and non-transgenic poplar (CK), and the screened ten pairs of SSR primers were used. The results showed that 76 alleles were observed in ten pairs of primers. The average allele was 7.6, the average effective number of alleles was 2.2, the average observed heterozygosity was 0.5167, the average expected heterozygosity was 0.5167, and the average percentage of polymorphic loci was 96.7%. The genetic diversity level of C. anachoreta experimental population fed on transgenic poplar 741 was significantly higher than that fed on non-transgenic populations, and C. anachoreta fed on high resistance had the lowest genetic similarity with CK samples, which showed an increasing trend of the genetic diversity of the experimental population fed on transgenic Bt poplar. It was thus clear that transgenic hybrid poplar 741 had stress effects on genetic differentiation of C. anachoreta experimental population by SSR.

刘军侠, 宋晓英, 姜文虎, 周国娜, 高宝嘉. 靶标昆虫杨扇舟蛾对转Bt基因741杨胁迫效应的SSR分析[J]. 应用生态学报, 2016, 27(12): 4039-4044.

LIU Jun-xia, SONG Xiao-ying, JIANG Wen-hu, ZHOU Guo-na, GAO Bao-jia. SSR analysis on stress effect of transgenic hybrid poplar 741 on Clostera anachoreta (Fabricius) (Lepidoptera: Notodontidae)[J]. Chinese Journal of Applied Ecology, 2016, 27(12): 4039-4044.

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靶标昆虫杨扇舟蛾对转Bt基因741杨胁迫效应的SSR分析

SSR analysis on stress effect of transgenic hybrid poplar 741 on Clostera anachoreta (Fabricius) (Lepidoptera: Notodontidae)

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