高效解磷细菌的筛选及其对玉米苗期生长的促进作用

应用生态学报

高效解磷细菌的筛选及其对玉米苗期生长的促进作用

朱培淼;杨兴明;徐阳春;欧阳红;沈其荣

南京农业大学资源与环境学院，南京 210095

收稿日期:2006-01-10 修回日期:2006-10-27 出版日期:2007-01-18 发布日期:2007-01-18

High effective phosphate-solubilizing bacteria: Their isolation and promoting effect on corn seedling growth

ZHU Pei-miao;YANG Xing-ming;XU Yang-chun;OUYANG Hong;SHEN Qi-rong

College of Resources and Environmental Sciences, Nanjing Agricultural University,
Nanjing 210095, China

Received:2006-01-10 Revised:2006-10-27 Online:2007-01-18 Published:2007-01-18

摘要/Abstract

摘要： 采用改良后的PVK平板，从石灰性土壤上长势良好的野生植物根表分离到44株解磷细菌，通过NBRIP液体摇瓶实验，培养7 d后发现：K₃菌株培养液中全磷浓度高达643.2 μg·ml^-1，可溶性磷为584.8 μg·ml^-1，约有12.9%的磷酸三钙被溶解出来，为对照(CK)的10.5倍；K₉菌株培养液的全磷浓度为608.5 μg·ml^-1，可溶性磷浓度为606.4 μg·ml^-1.盆栽试验的结果表明：接种解磷细菌的处理玉米株高、茎粗和干质量显著高于CK；将有机肥作为载体和解磷细菌一同混合施入土壤的处理，玉米苗干质量较单施解磷菌显著增加.经初步鉴定, K₃、K₉为假单胞菌属.

关键词: 空间多样性指数, 野生动物, 生境格局, 辽河三角洲

Abstract: By using modified PVK plate method, 44 strains of phosphate-solubilizing bacteria (PSB) were isolated from the root surface of wild plants growing well in calcareous soil. After 7-day incubation in a liquid medium NBRIP, the total P in the cultures of strains K₃ and K₉ reached 643.2 μg·ml^-1 and 608.5 μg·ml^-1, and soluble P was 584.8 μg·ml^-1 and 606.4 μg·ml^-1, respectively. About 12.9% of tricalcium phosphate was dissolved, being 9.5 times higher than CK. Pot experiment showed that the plant height, stem diameter, and dry mass of corn seedling were significantly higher in PSB treatments than in CK. Applying PSB agent with manure as a carrier could significantly increase the seedling’s dry mass, in comparing with applying PSB agent alone. The strains K₃ and K₉ were identified as Pseudomonas.

Key words: Spatial diversity index, Wildlife, Habitat pattern, Liaohe Delta

朱培淼;杨兴明;徐阳春;欧阳红;沈其荣. 高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J]. 应用生态学报.

ZHU Pei-miao;YANG Xing-ming;XU Yang-chun;OUYANG Hong;SHEN Qi-rong. High effective phosphate-solubilizing bacteria: Their isolation and promoting effect on corn seedling growth[J]. Chinese Journal of Applied Ecology.

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