瓜类保护地土壤镰孢菌种群及UP-PCR多样性分析

应用生态学报

瓜类保护地土壤镰孢菌种群及UP-PCR多样性分析

赵柏霞;高增贵;庄敬华;张小飞;赵辉

沈阳农业大学植物保护学院农业部北方农作物病害免疫重点开放实验室，沈阳 110161

收稿日期:2008-10-10 修回日期:1900-01-01 出版日期:2009-04-20 发布日期:2009-04-20

UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils.

ZHAO Bai-xia;GAO Zeng-gui;ZHUANG Jing-hua;ZHANG Xiao-fei;ZHAO Hui

Ministry of Agriculture Key Laboratory of Northern Crop Immunology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161,China

Received:2008-10-10 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20

摘要/Abstract

摘要： 对采自辽宁省部分地区瓜类保护地的36份土壤样品进行镰孢菌（Fusarium）分离培养，共获得112株镰孢菌，采用传统形态学分类和现代分子生物学方法，确定属于11个种.对其中25株镰孢菌及3株对照镰孢菌进行了通用引物PCR（UP-PCR）多样性分析.结果表明：6条引物扩增出73条带，其中多态性条带66条，占总条带数的90.4%.对供试菌株进行UP-PCR聚类图谱分析，当相似系数为0.736时，可将其划分为8个类群，其中14株尖孢镰孢菌聚为一类.UP-PCR分析体现了镰孢菌菌株间的亲缘关系及差异性，可以作为镰孢菌分类的辅助方法.

关键词: 节肢动物, 林分类型, 种-多度, 种-面积, 恢复区

Abstract: A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR (UP-PCR) was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fu sarium isolates. The results indicated that a total of 73 bands appeared after amp lification by using 6 primers, and 66 bands (904%) were polymorphic. The isola tes were clustered into eight groups at the similarity of 0736 by cluster anal ysis, among which, 14 isolates were clustered into one group. It was concluded t hat UP-PCR could present the genetic relationship and difference among Fusari um strains, being able to be used as an assistant method for Fusarium clas sificati on.

Key words:
arthropod, forest stand type, species-abundance, species-area, restoration area.

赵柏霞;高增贵;庄敬华;张小飞;赵辉. 瓜类保护地土壤镰孢菌种群及UP-PCR多样性分析[J]. 应用生态学报.

ZHAO Bai-xia;GAO Zeng-gui;ZHUANG Jing-hua;ZHANG Xiao-fei;ZHAO Hui. UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils.[J]. Chinese Journal of Applied Ecology.

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