枸杞红瘿蚊对寄主植物挥发物的电生理和行为反应

doi:10.13287/j.1001-9332.202007.040

应用生态学报 ›› 2020, Vol. 31 ›› Issue (7): 2299-2306.doi: 10.13287/j.1001-9332.202007.040

枸杞红瘿蚊对寄主植物挥发物的电生理和行为反应

张凡¹, 徐常青¹, 陈君¹, 马妹², 陆鹏飞², 刘赛¹, 李建领^1,3, 乔海莉^1*

¹中国医学科学院/北京协和医学院药用植物研究所, 北京 100193;
²北京林业大学省部共建森林培育与保护教育部重点实验室, 北京 100083;
³华润三九医药股份有限公司, 广东深圳 518110

收稿日期:2019-12-31 接受日期:2020-04-21 出版日期:2020-07-15 发布日期:2021-01-15
通讯作者: E-mail: qhl193314@sina.com
作者简介:张凡, 女, 1990年生, 硕士研究生。主要从事药用植物病虫害防治研究。E-mail: xiaofanzhang94@163.com
基金资助:
国家自然科学基金项目(81774015,81673699,31570643)和中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017)资助

Electrophysiological and behavioral responses of gall midge, Jaapiella sp. to volatiles of host plant Lycium barbarum

ZHANG Fan¹, XU Chang-qing¹, CHEN Jun¹, MA Mei², LU Peng-fei², LIU Sai¹, LI Jian-ling^1,3, QIAO Hai-li^1*

¹Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China;
²Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China;
³China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518110, Guangdong, China

Received:2019-12-31 Accepted:2020-04-21 Online:2020-07-15 Published:2021-01-15
Contact: E-mail: qhl193314@sina.com
Supported by:
This work was supported by the National Natural Science Foundation of China (81774015, 81673699, 31570643) and the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (2016-I2M-3-017).

摘要/Abstract

摘要： 植物挥发物是植食性昆虫定位寄主的重要信息物质。枸杞红瘿蚊是宁夏枸杞的主要成灾害虫,野外发现其雌虫对枸杞幼嫩花蕾具有更强的产卵趋性。为明确枸杞红瘿蚊对枸杞不同阶段花蕾挥发物的选择差异性,解析吸引其选择产卵的关键信息化学物质,采用顶空固相微萃取法和气相色谱-质谱联用技术(GC-MS),系统研究枸杞幼嫩花蕾和成熟花蕾挥发物的成分差异,选择幼嫩花蕾挥发物中的特有成分及其与成熟花蕾挥发物共有成分共8种作为候选挥发物;通过气相色谱-触角电位联用仪(GC-EAD)和Y型嗅觉仪,测定枸杞红瘿蚊对上述挥发物的电生理和行为反应,筛选关键活性挥发物。结果显示: 枸杞幼嫩花蕾和成熟花蕾挥发物的种类和释放量存在明显差异;8种化合物能激发枸杞红瘿蚊产生持续触角电生理反应,其中,有5种[3-蒈烯、莰烯、萜品油烯、d-柠檬烯和(+)-α-蒎烯]能刺激枸杞红瘿蚊产生显著趋向或躲避行为。利用枸杞挥发物设计枸杞红瘿蚊引诱剂或忌避剂,可为田间安全防控红瘿蚊提供新的途径。

关键词: 枸杞红瘿蚊, 植物挥发物, SPME, GC-MS, GC-EAD, Y型嗅觉仪

Abstract: Plant-derived volatiles are important for guiding herbivorous insects to their host plants. Jaapiella sp. (Diptera: Cecidomyiidae) is the most serious pest of Lycium barbarum. The young flower buds of L. barbarum are attractive to females of Jaapiella sp. for oviposition in the field. In this study, we compared response of Jaapiella sp. to volatiles from flower buds of L. barbarum at different stages, and clarified the crucial semiochemicals attracting Jaapiella sp. to lay eggs. Volatiles from young and mature flower buds were collected using SPME, and then identified and quantified by gas chromatography-mass spectrometry (GC-MS). Both electrophysiological and behavior experiments were conducted to measure the attraction of eight synthetic compounds to females and screen the crucial components based on gas chromatography-electroantennogram detection (GC-EAD) and Y-tube bioassays, respectively. Results showed that qualitative and quantitative diffe-rences between two odor profiles. Consistent electroantennographic response was observed for eight compounds from headspace collections, of which five synthetic compounds (3-carene, camphene, terpinolene, d-limonene, and (+)-pinene) were essential for significant preference or avoidance. Our results indicated that these compounds from L. barbarum flower buds could be effective candidates as oviposition attractant or repellents of Jaapiella sp., which would be helpful in the exploitation of new control agent to Jaapiella sp. in the field.

Key words: Jaapiella sp., plant olatiles, SPME, GC-MS, GC-EAD, Y-tube olfactometer

张凡, 徐常青, 陈君, 马妹, 陆鹏飞, 刘赛, 李建领, 乔海莉. 枸杞红瘿蚊对寄主植物挥发物的电生理和行为反应[J]. 应用生态学报, 2020, 31(7): 2299-2306.

ZHANG Fan, XU Chang-qing, CHEN Jun, MA Mei, LU Peng-fei, LIU Sai, LI Jian-ling, QIAO Hai-li. Electrophysiological and behavioral responses of gall midge, Jaapiella sp. to volatiles of host plant Lycium barbarum[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2299-2306.

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枸杞红瘿蚊对寄主植物挥发物的电生理和行为反应

Electrophysiological and behavioral responses of gall midge, Jaapiella sp. to volatiles of host plant Lycium barbarum

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