鼠尾藻对赤潮异弯藻和中肋骨条藻的抑制作用

应用生态学报

鼠尾藻对赤潮异弯藻和中肋骨条藻的抑制作用

王仁君¹;唐学玺¹;冯蕾^1,2;肖慧¹;曲良¹;蔡恒江¹

¹中国海洋大学海洋生态学研究室，青岛 266003；
²东营职业学院，山东东营 257091

收稿日期:2005-11-28 修回日期:2006-09-28 出版日期:2006-12-18 发布日期:2006-12-18

Inhibitory effect of Surgassum thunbergii on Heterosigma akashiwo and Skeletonema costatum

WANG Renjun¹;TANG Xuexi¹; FENG Lei^{1, 2}; XIAO Hui¹;QU Liang¹;CAI Hengjiang¹

¹Laboratory of Marine Ecology, Ocean University of China, Qingdao, 266003, Shandong， China;
²Dongying Vocation College, Dongying, 257091, Shandong， China

Received:2005-11-28 Revised:2006-09-28 Online:2006-12-18 Published:2006-12-18

摘要/Abstract

摘要： 通过共培养的方法，研究了鼠尾藻（Surgassum thunbergii）培养水的过滤液、新鲜组织、干粉末、水溶性抽提液对赤潮异弯藻（Heterosigma akashiwo）和中肋骨条藻（Skeletonema costatum）生长的抑制效应，建立了分隔共培养系统并证明了抑制物质的存在，排除了细胞直接接触抑制的可能性.结果表明，在共培养实验中，鼠尾藻新鲜组织、干粉末及水溶性抽提液对赤潮异弯藻和中肋骨条藻的生长具有显著的抑制作用，且在较高浓度下对两种赤潮微藻的生长具有致死效应.在一次性及半连续培养方式下，鼠尾藻培养水过滤液对赤潮异弯藻的生长无抑制效应，而对中肋骨条藻的生长具有显著的抑制效应.

Abstract: By the method of co-culture, this paper studied the inhibitory effect of Surgassum thunbergii culture filtrate, fresh tissue, dry powder, and aqueous extract on the growth of Heterosigma akashiwo and Skeletonema costatum. An isolated co-culture system was built, which confirmed the existence of allelochemicals and precluded the growth inhibition by direct contact. The results showed that the fresh tissue, dry powder, and aqueous extract of S. thunbergii could significantly inhibit the growth of H. akashiwo and S. costatum, and had lethal effect at relatively higher concentrations. The culture filtrate of S. thunbergii had no inhibitory effect on H. akashiwo, but could significantly inhibit the growth of S. costatum under initial or semi-continuous filtrate addition.

Key words: Cd²⁺, Pollution of heavy metal, Alternanthera philoxeroides, Antioxidant enzyme systems, Ultrastructure

王仁君¹;唐学玺¹;冯蕾^1,2;肖慧¹;曲良¹;蔡恒江¹. 鼠尾藻对赤潮异弯藻和中肋骨条藻的抑制作用[J]. 应用生态学报.

WANG Renjun¹;TANG Xuexi¹; FENG Lei^{1, 2}; XIAO Hui¹;QU Liang¹;CAI Hengjiang¹ . Inhibitory effect of Surgassum thunbergii on Heterosigma akashiwo and Skeletonema costatum[J]. Chinese Journal of Applied Ecology.