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应用生态学报 ›› 2011, Vol. 22 ›› Issue (02): 357-363.

• 研究报告 • 上一篇    下一篇

法国冬青和光皮桦挥发物日节律及云斑天牛的触角电位反应

杨桦1,2,杨伟1**,杨茂发2,杨春平1,杨令国3,徐唐鑫科3   

  1. 1四川农业大学森林保护重点实验室, 四川雅安 625014|2贵州大学昆虫研究所贵州山地农业病虫害重点实验室, 贵阳 550025|3罗江县林业局, 四川罗江 618500
  • 出版日期:2011-02-18 发布日期:2011-02-18

Diurnal rhythm of Viburnum awabuki and Betula luminifera volatiles and electroantennogram response of Batocera horsfieldi.

YANG Hua1,2, YANG Wei1, YANG Mao-fa2, YANG Chun-ping1, YANG Ling-guo3, XUTANG Xin-ke3   

  1. 1Key Laboratory of Forest Conservation, Sichuan Agricultural University, Ya’an 625014, Sichuan, China|2Key Laboratory for Plant Pest Management of Mountain Region, Institute of Entomology, Guizhou University, Guiyang 550025, China|3Luojiang Forestry Bureau, Luojiang 618500, Sichuan, China
  • Online:2011-02-18 Published:2011-02-18

摘要: 采用Tenax-TA吸附剂吸附法结合气相色谱-质谱联用技术(GC-MS)分析了法国冬青和光皮桦的健康株和云斑天牛危害株挥发性物质的日节律变化,并利用触角电位技术比较分析了云斑天牛未交配雌、雄虫对法国冬青和光皮桦挥发物的触角电位(EAG)反应.结果表明: 法国冬青被取食后柠檬烯、壬醛、十六烷、丙烯酸丁酯和3-甲基丁酸的含量减少,光皮桦被取食后新己烷和十六烷的含量减少;而法国冬青和光皮桦被取食后新合成了异十二烯、辛醇、吲哚、癸醛、己醛和丙烯菊酯等物质.云斑天牛未交配雌、雄成虫对光皮桦被害株挥发物的EAG反应值最大,分别为1.23和1.38 mV;对法国冬青健康株挥发物的EAG反应值最小,分别为0.95和1.01 mV.不同时段,成虫对12:00—14:00挥发成分的EAG反应值最低,与成虫野外取食行为多发生在清晨和傍晚相一致.

关键词: 光皮桦, 植物挥发物, 日节律, 生理状态, 触角电位反应, 法国冬青

Abstract: By the method of Tenax-TA absorbent adsorption combined with GC-MS, this paper analyzed the changes of the diurnal rhythm of the volatiles in the healthy branches and Batocera horsfieldi-damaged branches of Viburnum awabuki and Betula luminifera, and electroantennogram technique was used to perform a comparative analysis on the electroantennogram (EAG) responses of unmated male and female B. horsfieldi to the volatiles. After the feeding by B. horsfieldi, there was a decrease in the contents of limonene, nonanal, hexadecane, butyl acrylate, and 3-methyl-butanoic acid in damaged branches of V. awabuki and in the neohexane and hexadecane contents in damaged branches of B. luminifera. Simultaneously, new materials such as permethyl 99A, octyl alcohol, iodo, decanal, hexanal, and bioallethrin etc. were newly synthesized in the dama ged branches. The EAG response values of unmated male and female B. horsfieldi adults to the volatiles in the damaged branches of B.luminifera were the highest, being 1.23 mV and 1.38 mV, while to the healthy branches of V. awa buki were the lowest, being 0.95 mV and 1.01 mV, respectively. As for the time period, the EAG response values of the adults to the volatiles were the lowest from 12:00 to 14:00, which accorded with the feeding behaviors of the adults, i.e., taking food in the field in early morning or at dusk.

Key words: Viburnum awabuki, Betula luminifera, plant volatiles, diurnal rhythm, physiological status,  , electroantennogram response