东北春大豆样本的代表性及其SSR位点的遗传多样性分析

应用生态学报 ›› 2005, Vol. 16 ›› Issue (8): 1469-1476.

东北春大豆样本的代表性及其SSR位点的遗传多样性分析

栾维江^1,2, 刘章雄¹, 关荣霞¹, 常汝镇¹, 何蓓如², 邱丽娟¹

1. 中国农业科学院作物科学研究所农业部作物种质资源与生物技术重点开放实验室, 北京 100081;
2. 西北农林科技大学家学院, 杨凌 712100

收稿日期:2004-10-03 修回日期:2005-02-21 出版日期:2005-08-15 发布日期:2005-08-15
通讯作者: 邱丽娟,E-mail:qiu-lijuan@263.net
基金资助:
国家重点基础研究发展规划项目(G1998010203,2004CB117203)和国家科技攻关资助项目(2004BA525B06).

Representativeness of Northeast China spring soybeans and their genetic diversity at SSR loci

LUAN Weijiang^1,2, LIU Zhangxiong¹, GUAN Rongxia¹, CHANG Ruzhen¹, HE Beiru², QIU Lijuan ¹

1. Key Laboratory of Crop Germplasm and Biotechnology of Agriculture Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China

Received:2004-10-03 Revised:2005-02-21 Online:2005-08-15 Published:2005-08-15

摘要/Abstract

摘要： 从3226份东北春大豆总体中选择283份春大豆种质,用质量性状和数量性状进行检测,对总体的代表性为80%.利用筛选出61对SSR核心引物对具代表性的东北春大豆样本进行分析,共检测到534个等位变异,平均每个位点的等位变异为8.75个,变幅为2～16个;遗传多样性指数变化范围在0.406～0.886,平均为0.704;东北春大豆样本在大多数位点上有优势等位变异,从而降低了其遗传多样性.其中35份种质具有特异等位变异,分布在29个位点上;各个位点上分化系数均较小,遗传多样性分化程度较低.东北春大豆中3个省种质的共有等位变异较多,以吉林省和辽宁省种质的遗传多样性表现较为一致,均高于黑龙江省种质的遗传多样性.地方品种的遗传多样性高于育成品种.东北春大豆种质资源的遗传多样性分布特点为有目的选择杂交亲本拓宽遗传基础以培育新品种提供了理论依据.

关键词: 东北春大豆, SSR标记, 遗传多样性, 等位变异, 核心种质

Abstract: A total of 283 accessions were selected from the total 3 226 Northeast China spring soybeans,which represented >80% of the whole based on their qualitative and quantitative traits.The representative samples were analyzed by 61 SSR loci,and a total of 534 alleles were detected,ranging 216 alleles per locus,with an average of 8.75 alleles per locus.Among the accessions,the Simpson diversity index (SDI) for each locus ranged from 0.406 to 0.886,with a mean of 0.704,which was relatively lower since there were dominant alleles at most of loci in the representative samples.35 accessions had specific alleles,which distributed among 29 loci.The differentiation coefficient was lower than 9.27% in 61 loci among the three provinces of Northeast China,which might be caused by lots of common alleles shared among these provinces.The genetic diversity in Jilin and Liaoning provinces was nearly equal,but was higher than that in Heilongjiang Province.The landraces of the representative samples at 61 loci had a higher genetic diversity than that of the released cultivars.The genetic diversity appeared within and between the provinces could be used to broaden the genetic base of modern cultivars,and also,the landraces were the major source for soybean breeding because of their high genetic diversity.

Key words: Northeast China spring soybean, SSR marks, Genetic diversity, Core collection

中图分类号:

S311

栾维江, 刘章雄, 关荣霞, 常汝镇, 何蓓如, 邱丽娟. 东北春大豆样本的代表性及其SSR位点的遗传多样性分析[J]. 应用生态学报, 2005, 16(8): 1469-1476.

LUAN Weijiang, LIU Zhangxiong, GUAN Rongxia, CHANG Ruzhen, HE Beiru, QIU Lijuan . Representativeness of Northeast China spring soybeans and their genetic diversity at SSR loci[J]. Chinese Journal of Applied Ecology, 2005, 16(8): 1469-1476.

参考文献

[1] Chang R-Z(常汝镇).1989.Study on genetic resources of soybean in China Ⅰ.Growth period of genetic resources of soybean in different cultural area.J Plant Genetic Res(作物品种资源),28(2):4～6(in Chinese)
[2] Chang R-Z(常汝镇).1989.Study on genetic resources of soybean in China Ⅱ.Traits of seed for soybean cultivars in different cultural area.J Plant Genetic Res(作物品种资原),30(4):11～14(in Chinese)
[3] Chang R-Z(常汝镇).1990.Study on genetic resources of soybean in China Ⅲ.Feature of distribution for growth habit and pods habit of soybean.J Plant Genetic Res(作物品种资源),32(2):1～2(in Chinese)
[4] Chang R-Z(常汝镇).1990.Study on genetic resources of soybean in China Ⅳ.Trait of plant for genetic resources of soybean in different area.J Plant Genetic Res(作物品种资源),32(2):10～11(in Chinese)
[5] Cui Y-H(崔艳华),Qiu L-J(邱丽娟),Chang R-Z(常汝镇),et al.2004.A study of genetic diversity of Huanghuai summer sowing soybean in China.Sci Agric Sin(中国农业科学),37(1):15～22(in Chinese)
[6] Edwards K,Johnstone C,Thompson C.1991.A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.Necleic Acid Res,19:1349
[7] Frankel OH,Brown AHD.1984.Current Plant Genetic Resources-A Critical Appraisal.New Delhi:Oxford and IBH Press.
[8] Frankel OH.1984.Genetic perspective of germplasm conservation.In:Arber WL,eds.Genetic Manipulation:Impact on Man and Society.Cambridge:Cambridge University Press.
[9] Gai J-Y(盖钧镒),Zhao T-J(赵团结),Cui Z-L(崔章林),et al.1999.The Genetic for 651 soybean cultivars released during 1923～1995 in China.Rev Chin Agric Sci Technol(中国农业科技导报),(1):26～31(in Chinese)
[10] Gai J-Y(盖钧镒),Xu D-H(许东河),Gao Z(高忠),et al.2000.Studies on the evolutionary relationship among eco-types of G.max and G.soja in China.Acta Agron Sin(作物学报),26(5):513～516(in Chinese)
[11] Heale SM,Petes TD.1995.The stabilization of repetitive tract of DNA by variant repeats requires a functional DNA mismatch repair system.Cell,83:539-545
[12] Institute of Crop Germplams Resources of Chinese Academy of Agricultural Sciences(中国农业科学院作物品种资源研究所).1991.Catalog of Chinese Soybean Germplam Resources(continued 1).Beijing:China Agriculture Press.1～60(in Chinese)
[13] Institute of Crop Germplams Resources of Chinese Academy of Agricultural Sciences(中国农业科学院作物品种资源研究所).1996.Catalog of Chinese Soybean Germplam Resources(continued 2).Beijing:China Agriculture Press.1～38(in Chinese)
[14] Institute of Oil Crop of Chinese Academy of Agricultural Sciences(中国农业科学院油料作物研究所).1980.Catalog of Chinese Soybean Germplam Resources.Beijing:China Agriculture Press.1～124(in Chinese)
[15] Kruglyak S,Durrett R,Schug MD,et al.2001.Distribution and abundance of microsatellites in the yeast genome can be explained by a balance between slippage events and point mutations.Mol Biol Evol,17(8):1210～1219
[16] Lin F-Y(林凡云),Qiu L-J(邱丽娟),Chang R-Z(常汝镇),et al.2003.Genetic diversity of landrace and bred varieties of soybean in Shanxi.Chin J Oil Crop Sci(中国油料作物学报),24(3):24～29(in Chinese)
[17] Li Z,Nelson RL.2001.Genetic diversity among soybean accessions from three countries mensured by RAPDs.Crop Sci.41:1337～1347
[18] Maughan PJ,Saghai Maroot MA,Buss GR.1995.Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean.Genome,38(4):715～723
[19] Powell W,Morgante M,Doyle JJ,et al.1996.Genepool variation in genus Glycine subgenus Soja revealed by polymorphic nuclear and chloroplast microsatellites.Genetics,144(2):793～803
[20] Qiu L-J(邱丽娟),Cao Y-S(曹永生),Chang R-Z(常汝),et al.2003.Establishment of Chinese soybean(G.max)core collection I.Sampling strategy.Sci Agric Sin(中国农业科学),36(12):1442～1449(in Chinese)
[21] Qiu L-J(邱丽娟),Nelson RL,Vodkin LO.1997.Evaluation of soybean germplasm with random amplification polymorphic DNA(RAPD)markers.Acta Agron Sin(作物学报),23(4):408～417(in Chinese)
[22] Schltterere C,Tautz D.1992.Slippage synthesis of simple sequence DNA.Nucl Acids Res,20:211～215
[23] Sun Z-Q(孙志强),Liu Y-Z(刘玉芝),Sun D-M(孙大敏).1990.Genetic analysis of resistance of soybean for 1,2 and 3 lines of soybean mosaic virus(SMV).Chin J Oil Crop Sci(中国油料),(2):20～24(in Chinese)
[24] WangB(王彪),ChangR-Z(常汝镇),TaoL(陶莉),et al.2003.Identification of SSR primer numbers for analyzing genetic diversity of Chinese soybean cultivated soybean.Mol Plant Breeding(分子植物育种),1(1):82～88(in Chinese)
[25] Wang J-L(王金陵),Yang Q-K(杨庆凯),Wu Z-P(吴宗璞).1994.Northeast Soybean of China.Harbin:Heilongjiang Science and Technology Press.144～232(in Chinese)
[26] Wang Z,Weber JL,Zhong G,et al.1994.Survey of plant short tandem DNA repeats.Ther Appl Genet,88:1～6
[27] Wu X-L(吴晓雷),He C-Y(贺超英),Chen S-Y(陈受宜),et al.2001.Phylogenetic analysis of interspecies in genus Glycine through SSR markers.Acta Genet Sin(遗传学报),28(4):359～366(in Chinese)
[28] Xie H(谢华),Chang R-Z(常汝镇),Cao Y-S(曹永生),et al.2003.Selection of core SSR loci by using Chinese autumn soybean(Glycine max L.Merr).Sci Agric Sin(中国农业科学),36(4):360～366(in Chinese)
[29] Yan P(晏鹏),Wu X-B(吴孝兵).2003.Technique of microsatellite polymorphism and its application in the protective genetics.Chin J Appl Ecol(应用生态学报),14(3):461～464(in Chinese)
[30] Zhang J-L(张军丽),Wang Z-F(王峥峰),Li M-G(李鸣光).2000.Molecular marker and its application in plant species.Chin J Appl Ecol(应用生态学报),11(4):631～636(in Chinese)