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田间条件下不同诱导棉花挥发性物质的鉴定

于惠林1,2;张永军1;潘文亮3;郭予元1;高希武2   

  1. 1中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京 100094;2中国农业大学农学与生物技术学院, 北京 100094;3河北农林科学院植物保护研究所, 河北保定 071000
  • 收稿日期:2006-03-16 修回日期:1900-01-01 出版日期:2007-04-05 发布日期:2007-04-05

Identification of volatiles from field cotton plant under different induction treatments.

YU Hui-lin1,2; ZHANG Yong-jun1; PAN Wen-liang3; GUO Yu-yuan1; GAO Xi-wu2   

  1. 1State Key Laboratory of Biology for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;2College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;3Institute of Plant Protection, Hebei Academy of Agriculture and Forestry, Baoding 071000, Hebei, China
  • Received:2006-03-16 Revised:1900-01-01 Online:2007-04-05 Published:2007-04-05

摘要: 采用人工机械损伤(处理A)、棉铃虫为害(处理B)、棉铃虫为害+棉铃虫体(处理C)和水杨酸诱导(处理D)4种处理方式,对田间现蕾期棉花植株挥发性化合物进行了定性和定量研究.结果表明:有30多种棉株挥发性化合物被定性检出,对其中10种主要挥发性物质进行定量分析,田间棉株挥发的绿叶气味化合物有3-己酮、2-己酮和3-己醇等;单萜类化合物有α-蒎烯、β-蒎烯、β-月桂烯、3,7-二甲基,1,3,6-辛三烯等;脂肪族化合物有丙酸丁酯、乙酸戊酯、乙酸丁酯和3-甲基丁酸乙酯;芳香族化合物有苯乙酮、苯甲醛等.处理C和处理B的棉花挥发信息化合物种类和释放量均明显多于对照,这两个处理检测到3,7-二甲基,1,3,6-辛三烯;而处理A和处理D未检测到该化合物.与对照相比,处理A挥发物在种类和含量上差异不显著.在田间,处理B、处理C和处理D均能诱导促进棉株挥发物的释放.

关键词: 颗粒悬浮物, 轮虫培育池生态系统

Abstract: The volatiles of field cotton plant at its squaring stage were analyzed qualitatively and quantitatively under treatments of mechanical injury (treatment A), cotton bollworm (CBW) injury (treatment B), CBW plus CBW larva injury (treatment C), and salicylic acid (SA) inducement (treatment D). The volatiles were gathered in an automatic circle system, absorbed by Tenax-TA column, and analyzed with GC-MS system. About 30 kinds of volatiles were qualitatively identified, including terpenoids, aliphatic compounds, aromatic compounds, 3-hexanone, 2-hexanone, 3-hexanol, a-pinene, β-pinene,β-myrcene, propenoic acid, butyl ester, acetic acid, pentyl ester, acetic acid , butyl ester, butanoic acid, 3-methyl-, ethyl ester, benzaldehyde, acetophenone and 1,3,6-octatriene,3,7-dimethyl, etc., and a total of 10 primary volatiles were detected quantitatively. The results showed that both the kinds and the contents of the volatiles were notably higher in treatments B and C than in the control. It was noteworthy that 1,3,6-octatriene 3,7-dimethyl was only found in treatments B and C, i. e. , it only occurred in the cotton plants injured by CBW.There were no significant differences in the kinds and contents of the volatiles between treatment A and the control. Similar to CBW injury, SA inducement also enhanced the release of cotton plant volatiles.

Key words: Suspending pariculates, Rotifer-culturing pond ecosystem