重茬胁迫下硒对大豆叶绿体超微结构和叶片中微量元素含量的影响

应用生态学报 ›› 2003, Vol. ›› Issue (4): 573-576.

重茬胁迫下硒对大豆叶绿体超微结构和叶片中微量元素含量的影响

于颖¹, 刘元英², 罗盛国², 彭显龙²

1. 中国科学院沈阳应用生态研究所陆地生态过程重点实验室, 沈阳 110016;
2. 东北农业大学, 哈尔滨 150030

收稿日期:2001-12-24 修回日期:2002-05-08 出版日期:2003-04-15
通讯作者: 刘元英
基金资助:
国家自然科学基金资助项目(39870470).

Effects of selenium on soybean chloroplast ultra-structure and microelement content of soybean leaves under continuous cropping stress

YU Ying¹, LIU Yuanying², LUO Shengguo², PENG Xianlong²

1. Insititute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
2. Northeast Agricultural University, Harbin 150030, China

Received:2001-12-24 Revised:2002-05-08 Online:2003-04-15

摘要/Abstract

摘要： 采用盆栽试验和田间小区试验方法,研究了低浓度施Se对重茬胁迫下大豆叶绿体超微结构及大豆叶片中Mg、Fe、Mn含量的影响.结果表明,当盆栽试验施Se量小于0.50mg·kg^-1,田间小区试验施Se量小于300g·hm^-2时,施Se都不同程度地提高了大豆叶片的Mg、Fe和Mn含量.在盆栽试验中,施Se量与大豆叶片中Mg、Fe的含量呈正比,而施Se量与大豆叶片中Mn含量之间的关系很难确定.同时,透射电镜照片显示,盆栽试验中重茬胁迫下,施Se可使大豆叶绿体膜结构保持完好,而不施Se对照的叶绿体发生膨胀,基粒消失,甚至转化成了黄化体.可以认为,低浓度施Se能缓解大豆重茬胁迫所造成的过氧化损伤,使大豆生长较为正常.

Abstract: Pot and field experiments were carried out to study the effect of low Se concentration on soybean chloroplast ultra-structure and on Mg, Fe, Mn and contents in soybean leaves under continuous cropping stress. The results showed that Se could certainly increase the contents of Mg, Fe and Mn in soybean leaves when the application rate of Se was below 0.50mg穔g^-1 in pot experiment, and below 300g穐m^-2 in field experiment.In pot experiment, Se concentration positively correlated with Mg and Fe contents in soybean leaves, but the relationship between Mn and Se was hardly to be quantified.Transmission electron microscopic observation indicated that under continuous cropping stress, application of Se could hold the soybean chloroplast membrane structure in good condition.When Se was not applied,the chloroplast swelled, and its grana disappeared, and even,transformed into etioplast.These experiments indicated that application of low Se could alleviate peroxidative stress resulting from continuous cropping, and keep the soybean grow normally.

中图分类号:

S565.1

于颖, 刘元英, 罗盛国, 彭显龙. 重茬胁迫下硒对大豆叶绿体超微结构和叶片中微量元素含量的影响[J]. 应用生态学报, 2003, (4): 573-576.

YU Ying, LIU Yuanying, LUO Shengguo, PENG Xianlong. Effects of selenium on soybean chloroplast ultra-structure and microelement content of soybean leaves under continuous cropping stress[J]. Chinese Journal of Applied Ecology, 2003, (4): 573-576.

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