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应用生态学报 ›› 2001, Vol. ›› Issue (6): 900-902.

• 研究论文 • 上一篇    下一篇

渤海水母体细菌的微生态分布及弧菌生物学特性

刘全永, 胡江春, 马成新, 薛德林, 王书锦   

  1. 中国科学院沈阳应用生态研究所, 沈阳, 110016
  • 收稿日期:2001-01-12 修回日期:2001-04-26 出版日期:2001-11-25
  • 通讯作者: 刘全永,男,1970年生,微生物学在读博士,主要从事海洋微生物资源开发与利用研究.E-mail:Liuqy@iae.ac.cn
  • 基金资助:
    辽宁省科委资助项目(05960810).

Bacteria distribution and biological characteristics of vibrios in jellyfish from Bohai Sea

LIU Quanyong, HU Jiangchun, MA Chengxin, XUE Delin, WANG Shujin   

  1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016
  • Received:2001-01-12 Revised:2001-04-26 Online:2001-11-25

摘要: 采用稀释平板法对我国辽宁渤海海域水母体中细菌的微生态分布进行了考察.结果表明,在渤海水母体中各部位均有腐生性细菌及弧菌生长,其中腐生性细菌主要存在于水母体表,而弧菌除存在于水母体表外,有的则能在水母体内深层生长.水母体细菌微生态研究结果表明,弧菌约占细菌总数的90%以上.通过改进TCBS培养基,从水母体中分离得到6株优势类群的细菌,对其菌落特征、菌体形态、生理生化特性进行了研究,它们都具备弧菌属的共同特点:革兰氏阴性,氧化酶阳性,兼性厌氧,TCBS培养基上能生长,对O/129敏感,初步鉴定为弧菌属.研究还发现,这几株菌都能产蛋白酶,其中JF2、JF4、JF5、JF6产蛋白酶的能力明显较强,它们极有可能是导致水母捕捞后快速解体腐败的主要原因.

关键词: 水母, 蛋白酶, 弧菌, 微生态, 海洋微生物资源

Abstract: The bacteria distribution in jellyfish from Bohai Sea was investigated with dilution plate method. The result shows that anaerobes commonly existed in each part of jellyfish body, while aerobes mainly subsisted on jellyfish epiderm or in its head. Vibrios accounted for over 90% of total bacteria. By ameliorating the TCBS medium, 6 strains of vibrios were obtained from jellyfish. Studies on colony features, strain morphology, growth as well as physiological and biochemical characteristics show that these strains had common features, such as gram negative, oxidase positive, facultative anaerobic and growing on TCBS medium, which are typical characters of vibrios. In addition, all the 6 strains of vibrios can produce proteinase. Among which, JF2, JF4, JF5 and JF 6 showed strong proteinase-producing ability, which were possibly the main cause resulting in the decaying of jellyfish after being captured.

Key words: Jellyfish, Proteinase, Vibrio, Microecology, Ocean microorganism resource

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