Effects of understory removal on soil microbial community composition in subtropical Phyllostachys edulis plantations

doi:10.13287/j.1001-9332.202109.034

Abstract

Abstract: We investigated the effects of understory removal on soil microbial community and soil physicochemical properties in a field experiment following random block design in subtropical moso bamboo (Phyllostachys edulis) plantations, which were widely contributed in middle subtropical area, aiming to assess the regulation mechanism of understory plants on soil microbial community. The results showed that understory removal significantly increased the contents of soil N, NO₃^--N, and soil available phosphorus, but decreased soil pH and the contents of soil NH₄⁺-N and soil phosphorus (TP). Moreover, understory removal decreased total and bacterial PLFAs (B) and increasing soil fungal PLFAs (F), resulting in a higher F/B ratio. Redundancy analysis showed that changes in fungal PLFAs caused by understory removal were mainly attributed to soil acidification, while changes in bacterial PLFAs caused by understory removal were mainly due to the decreases in soil TP and pH. Furthermore, i14:0、i15:0 and i16:0 contributed to the decreases in bacterial biomass. Our results suggested that understory removal might not be suitable for the management of subtropical P. edulis plantations, as it would alter microbial community composition. The shift of soil microbial community from bacteria to fungi could inhibit microbial decomposition function.

Key words: understory removal, microbial community, phospholipid fatty acid, moso bamboo, forest management

XIAO Yi, CHEN Hui-xian, QIU Li-jun, ZHANG Yang, WAN Song-ze. Effects of understory removal on soil microbial community composition in subtropical Phyllostachys edulis plantations[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3089-3096.

References

[1] 朱永官, 沈仁芳, 贺纪正, 等. 中国土壤微生物组: 进展与展望. 中国科学院院刊, 2017, 32(6): 554-565 [Zhu Y-G, Shen R-F, He J-Z, et al. China soil microbiome initiative: Progress and perspective. Bulletin of Chinese Academy of Sciences, 2017, 32(6): 554-565]
[2] 郑蔚, 周嘉聪, 林伟盛, 等. 土壤增温对亚热带杉木幼树不同深度土壤微生物胞外酶活性的影响. 应用生态学报, 2019, 30(3): 832-840 [Zheng W, Zhou J-C, Lin W-S, et al. Effects of soil warming on soil microbial extracellular enzyme activities with different depths in a young Chinese fir (Cunninghamia lanceolata) plantation of subtropics. Chinese Journal of Applied Ecology, 2019, 30(3): 832-840]
[3] Wan SZ, Zhang CL, Chen YQ, et al. Interactive effects of understory removal and fertilization on soil respiration in subtropical Eucalyptus plantations. Journal of Plant Ecology, 2015, 8: 284-290
[4] Wardle DA, Bardgett RD, Klironomos JN, et al. Ecological linkages between aboveground and belowground biota. Science, 2004, 304: 1629-1633
[5] Bardgett RD, Bowman WD, Kaufmann R, et al. A temporal approach to linking aboveground and belowground ecology. Trends in Ecology & Evolution, 2005, 20: 634-641
[6] Zak DR, Holmes WE, White DC, et al. Plant diversity, soil microbial communities, and ecosystem function: Are there any links? Ecology, 2003, 84: 2042-2050
[7] De Deyn GB, Raaijmakers CE, Rik ZH, et al. Soil invertebrate fauna enhances grassland succession and diversity. Nature, 2003, 422: 711-713
[8] Packer A, Clay K. Soil pathogens and spatial patterns of seedling mortality in a temperate tree. Nature, 2000, 404: 278-281
[9] Wu JP, Liu ZF, Wang XL, et al. Effects of understory removal and tree girdling on soil microbial community composition and litter decomposition in two Eucalyptus plantations in South China. Functional Ecology, 2011, 25: 921-931
[10] Maina GG, Brown JS, Gersani M. Intra-plant versus inter-plant root competition in beans: Avoidance, resource matching or tragedy of the commons. Plant Ecology, 2002, 160: 235-247
[11] Caldwell JM, Sucoff EI, Dixon RK. Grass interference limits resource availability and reduces growth of juvenile red pine in the field. New Forests, 1995, 10: 1-15
[12] 胡亚林, 汪思龙, 颜绍馗. 影响土壤微生物活性与群落结构因素研究进展. 土壤通报, 2006, 37(1): 170-176 [Hu Y-L, Wang S-L, Yan S-K, et al. Research advances on the factors influencing the activity and community structure of soil microorganism. Chinese Journal of Soil Science, 2006, 37(1): 170-176]
[13] Osburn ED, Elliottt KJ, Knoepp JD, et al. Soil microbial response to Rhododendron understory removal in sou-thern Appalachian forests: Effects on extracellular enzymes. Soil Biology and Biochemistry, 2018, 127: 50-59
[14] Xiong Y, Xia H, Li ZA, et al. Impacts of litter and understory removal on soil properties in a subtropical Acacia mangium plantation in China. Plant and Soil, 2008, 304: 179-188
[15] Urcelay C, Díaz S, Gurvich DE, et al. Mycorrhizal community resilience in response to experimental plant functional type removals in a woody ecosystem. Journal of Ecology, 2009, 97: 1291-1301
[16] Wan SZ, Liu ZF, Chen YQ, et al. Effects of lime application and understory removal on soil microbial communities in subtropical Eucalyptus L'Hér. plantations. Forests, 2019, 10: 338
[17] 李玉敏, 冯鹏飞. 基于第九次全国森林资源清查的中国竹资源分析. 世界竹藤通讯, 2019, 17(6): 45-48 [Li Y-M, Feng P-F. Bamboo resources in China based on the Ninth National Forest Inventory Data. World Bamboo and Rattan, 2019, 17(6): 45-48]
[18] 陈阳, 王新杰. 闽西北丘陵地毛竹林下植物多样性的研究. 中南林业科技大学学报, 2014, 34(1): 84-88 [Chen Y, Wang X-J. Plant diversity under Phyllostachys pubescens forests in hilly area of northwest Fujian Pro-vince. Journal of Central South University of Forestry & Technology, 2014, 34(1): 84-88]
[19] 李光敏, 陈伏生, 徐志文, 等. 间伐和林下植被剔除对毛竹林土壤氮矿化速率及其温度敏感性的影响. 生态学报, 2019, 39(11): 4106-4115 [Li G-M, Chen F-S, Xu Z-W, et al. Effects of thinning and understory removal on soil nitrogen mineralization. Acta Ecologica Sinica, 2019, 39(11): 4106-4115]
[20] Wang FM, Li J, Zou B, et al. Effect of prescribed fire on soil properties and N transformation in two vegetation types in South China. Environmental Management, 2013, 51: 1164-1173
[21] Zhao J, Wan SZ, Fu SL, et al. Effects of understory removal and nitrogen fertilization on soil microbial communities in Eucalyptus plantations. Forest Ecology and Management, 2013, 310: 80-86
[22] 陈淼, 李小娟, 陈歆, 等. 不同施肥处理下热带土壤硝态氮累积特征及与土壤pH值、辣椒产量的关系. 西南农业学报, 2018, 31(5): 1045-1050 [Chen M, Li X-J, Chen X, et al. Characteristics of nitrate nitrogen accumulation in tropical soil and its relationship with pH and chilli yield under different fertilization treatments. Southwest China Journal of Agricultural Sciences, 2018, 31(5): 1045-1050]
[23] 沈灵凤, 白玲玉,曾希柏,等. 施肥对设施菜地土壤硝态氮累积及pH的影响. 农业环境科学学报, 2012, 31(7): 1350-1356 [Shen L-F, Bai L-Y, Zeng X-B, et al. Effects of fertilization on NO₃^--N accumulation in greenhouse soils. Journal of Agro-Environment Science, 2012, 31(7): 1350-1356]
[24] 佟德利, 徐仁扣. 三种氮肥对红壤硝化作用及酸化过程影响的研究. 植物营养与肥料学报, 2012, 18(4) 853-859 [Tong D-L, Xu R-K. Effects of urea, (NH₄)₂SO₄ and NH₄HCO₃ on nitrification and acidification of a red soil. Journal of Plant Nutrition and Fertilizers, 2012, 18(4): 853-859]
[25] Liu R, Zhang Y, Hu X, et al. Litter manipulation effects on microbial communities and enzymatic activities vary with soil depth in a subtropical Chinese fir plantation. Forest Ecology and Management, 2021, 480, doi.org/10.1016/j.foreco.2020.118641
[26] Winsome T, Silva LCR, Scow KM, et al. Plant-microbe interactions regulate carbon and nitrogen accumulation in forest soils. Forest Ecology and Management, 2017, 384: 415-423
[27] Murugan R, Beggi F, Kumar S. Belowground carbon allocation by trees, understory vegetation and soil type alter microbial community composition and nutrient cycling in tropical Eucalyptus plantations. Soil Biology and Biochemistry, 2014, 76: 257-267
[28] Song QN, Lu H, Liu J, et al. Accessing the impacts of bamboo expansion on NPP and N cycling in evergreen broadleaved forest in subtropical China. Scientific Reports, 2017, 7: 723-728
[29] 韩桂云, 齐玉臣, 刘忱, 等. 温度 pH对菌根真菌生长影响的研究. 生态学杂志, 1993, 12(1): 15-19 [Han G-Y, Qi Y-C, Liu C, et al. Effects of temperature and pH on mycorrhizal fungus growth. Chinese Journal of Ecology, 1993, 12(1): 15-19]
[30] 王立超, 连岩, 张志新. 温度、光照和pH之间的相互关系对光和细菌生长的影响. 海洋湖沼通报, 1997, 5(4): 28-31 [Wang L-C, Lian Y, Zhang Z-X. The effect of the relationships among temperature, light and pH on the growth of PSB. Transactions of Oceanology and Limnology, 1997, 5(4): 28-31]
[31] 严海元, 辜夕容, 申鸿. 森林凋落物的微生物分解. 生态学杂志, 2010, 29(9): 1827-1835 [Yan H-Y, Gu X-R, Shen H. Microbial decomposition of forest litter: A review. Chinese Journal of Ecology, 2010, 29(9): 1827-1835]
[32] Thirukkumaran CM, Parkinson D. Microbial respiration, biomass, metabolic quotient and litter decomposition in a lodgepole pine forest floor amended with nitrogen and phosphorous fertilizers. Soil Biology and Biochemistry, 2000, 32: 59-66
[33] Cox F, Barsoum N, Lilleskov EA, et al. Nitrogen availa-bility is a primary determinant of conifer mycorrhizas across complex environmental gradients. Ecology Letters, 2010, 13: 1103-1113
[34] Wu JP, Liu ZF, Chen DM, et al. Understory plants can make substantial contributions to soil respiration: Evidence from two subtropical plantations. Soil Biology and Biochemistry, 2011, 43: 2355-2357
[35] Thomas S, Katharina MK, Emanuel S, et al. Who is who in litter decomposition? Metaproteomics reveals major microbial players and their biogeochemical functions. The ISME Journal, 2012, 6: 1749-1762
[36] Dong WY, Zhang XY, Liu XY, et al. Responses of soil microbial communities and enzyme activities to nitrogen and phosphorus additions in Chinese fir plantations of subtropical China. Biogeosciences, 2015, 12: 5537-6646
[37] Trentini CP, Villagra M, Pámies DG, et al. Effect of nitrogen addition and litter removal on understory vegetation, soil mesofauna, and litter decomposition in loblolly pine plantations in subtropical Argentina. Forest Ecology and Management, 2018, 429: 133-142