Simulated nitrogen deposition reduces potential nitrous oxide emissions in a natural Castanopsis carlesii forest soil

doi:10.13287/j.1001-9332.202210.005

Abstract

Abstract: The reactive nitrogen deposition in subtropical region of China has been increasing annually, which affects biogeochemical processes in forest soils. In this study, three treatments were established, including control (no N addition, CK), low nitrogen deposition (40 kg·hm^-2·a^-1, LN), and high nitrogen deposition (80 kg·hm^-2·a^-1, HN) to study the response of denitrifying functional genes and potential N₂O emissions to simulated nitrogen deposition in the soils of a natural Castanopsis carlesii forest. Results showed that HN significantly decreased soil potential N₂O emission, while 8-year nitrogen deposition did not affect the abundances of nirS, nirK, nosZ Ⅰ and nosZ Ⅱ. However, the abundance of nosZ Ⅰwas significantly higher than nosZ Ⅱ in all the treatments, indicating that nosZ Ⅰ dominated over nosZ Ⅱ in the acidic soils. HN significantly decreased the ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which was positively correlated with soil pH. The results suggested that long-term high nitrogen deposition reduced soil pH and the abundance ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which subsequently reduced the potential N₂O emission.

Key words: nitrogen addition, forest soil, N₂O emission, denitrifying gene

DENG Mi-lin, FENG Meng-meng, LIU Xiao-fei, CHEN Shi-dong, HE Ji-zheng, LIN Yong-xin. Simulated nitrogen deposition reduces potential nitrous oxide emissions in a natural Castanopsis carlesii forest soil[J]. Chinese Journal of Applied Ecology, 2022, 33(10): 2705-2710.

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