Effects of long-term fertilization patterns on bacterial community structure and soil nutrients in dryland of yellow soil

doi:10.13287/j.1001-9332.202307.011

Abstract

Abstract: Understanding the responses of soil bacterial community to long-term fertilization in dryland of yellow soil could provide theoretical basis for establishing scientific fertilization system and cultivating healthy soil. Based on a 25-year long-term fertilization experiment on yellow soil, we collected soil samples from 0-20 cm layer under different fertilization treatments: no fertilization (CK), balanced application of N, P and K fertilizers (NPK), single application of organic fertilizer (M), combined application of constant organic and inorganic fertilizer (MNPK), and 1/2 organic fertilizer instead of 1/2 chemical fertilizer (MNP). Illumina MiSeq high-throughput sequencing technology was used to examine the effects of different fertilization patterns on soil bacterial community structure and soil nutrient content. The main driving factors of soil bacterial community were explored. The results showed that soil pH and organic matter content under treatments with organic fertilizer increased by 11.4%-13.5% and 28.8%-52.0%, respectively, compared to that under NPK treatment. Long-term fertilization did not affect soil bacterial α diversity, but significantly affected soil bacterial β diversity. Compared with CK and NPK treatment, treatments of M, MNP, and MNPK significantly changed soil bacterial community structure, and increased the relative abundance of Fusobacteria and Anaerobes. Four fertilization treatments increased the relative abundance of Bacteroidetes, and decreased the relative abundance of Actinomyces and Campylobacter, compared to CK. Soil pH was the most important factor affecting soil bacterial community structure. Fertilization-stimulated rare microbial taxa (Pumilomyces and Anaerobes) were more sensitive to changes in different environmental factors and were the main drivers of the formation of community versatility. In conclusion, organic fertilizer improved soil properties and fertility and changed soil bacterial community structure, which are conducive to cultivating healthy soil.

Key words: long-term fertilization, bacterial diversity, healthy soil

XIONG Han, LIU Yanling, LI Yu, ZHANG Yarong, HUANG Xingcheng, YANG Yehua, ZHU Huaqing, JIANG Taiming. Effects of long-term fertilization patterns on bacterial community structure and soil nutrients in dryland of yellow soil[J]. Chinese Journal of Applied Ecology, 2023, 34(7): 1949-1956.

References

[1] Carvalho FP. Pesticides, environment, and food safety. Food Energy Security, 2017, 6: 48-60
[2] Chen J, Luo YQ, Van G, et al. A keystone microbial enzyme for nitrogen control of soil carbon storage. Science Advances, 2018, 4: 1689
[3] 胡晓婧. 东北黑土区不同地域土壤微生物对长期施肥管理的响应研究. 博士论文. 北京: 中国科学院大学, 2018
[4] 武俊男. 长期不同施肥对玉米根际微生物多样性及功能菌群的影响. 硕士论文. 长春: 吉林农业大学, 2018
[5] 单鸿宾. 新疆棉田土壤微生物特性研究. 硕士论文. 乌鲁木齐: 新疆农业大学, 2009
[6] 林新坚, 王飞, 蔡海松, 等. 不同有机肥源对土壤微生物生物量及花生产量的影响. 中国生态农业学报, 2009, 17(2): 235-238
[7] 秦杰, 姜昕, 周晶, 等. 长期不同施肥黑土细菌和古菌群落结构及主效影响因子分析. 植物营养与肥料学报, 2015, 21(6): 1590-1598
[8] 王慧颖, 徐明岗, 周宝库, 等. 黑土细菌及真菌群落对长期施肥响应的差异及其驱动因素. 中国农业科学, 2018, 51(5): 914-925
[9] 王慧颖. 长期不同施肥下我国典型农田土壤细菌和真菌群落的特征. 硕士论文. 北京: 中国农业科学院, 2018
[10] 练金山, 王慧颖, 徐明岗, 等. 长期施用有机肥潮土细菌的多样性及功能预测. 植物营养与肥料学报, 2021, 27(12): 2073-2082
[11] 蔡泽江, 孙楠, 王伯仁, 等. 长期施肥对红壤pH、作物产量及氮、磷、钾养分吸收的影响. 植物营养与肥料学报, 2011, 17(1): 71-78
[12] 刘佳, 陈晓芬, 刘明, 等. 长期施肥对旱地红壤细菌群落的影响. 土壤学报, 2020, 57(2): 468-478
[13] 吕倩倩. 长期施肥对褐土酶活性及微生物多样性的影响. 硕士论文. 太原: 山西大学, 2020
[14] 刘彦伶, 李渝, 张雅蓉, 等. 长期施肥对黄壤稻田和旱地土壤磷酸酶活性的影响. 土壤通报, 2022, 53(4): 948-955
[15] 李渝, 刘彦伶, 白怡婧, 等. 黄壤稻田土壤微生物生物量碳磷对长期不同施肥的响应. 应用生态学报, 2019, 30(4): 1327-1334
[16] 张萌, 刘彦伶, 魏全全, 等. 酒糟生物炭短期施用对贵州黄壤氮素有效性及细菌群落结构多样性的影响. 环境科学, 2020, 41(10): 4690-4700
[17] 孙益权, 王美艳, 张龙, 等. “泡沫砂”改良黏重黄壤孔隙结构的粒径效应. 土壤, 2020, 52(3): 597-602
[18] 刘彦伶, 李渝, 张艳, 等. 长期施用磷肥和有机肥黄壤微生物量磷特征. 中国农业科学, 2021, 54(6): 1188-1198
[19] 柳玲玲, 李渝, 蒋太明, 等. 长期不同施肥措施对黄壤微生物量碳及氮的影响. 西南农业学报, 2017, 30(3): 645-649
[20] 杨叶华, 黄兴成, 朱华清, 等. 长期有机与无机肥配施的黄壤稻田土壤细菌群落结构特征. 植物营养与肥料学报, 2022, 28(6): 984-992
[21] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2007
[22] 陈浩宁, 周怀平, 文永莉, 等. 长期不同施肥下褐土养分及酶活性的生态化学计量特征. 植物营养与肥料学报, 2022, 28(6): 972-983
[23] Chaer G, Fernandes M, Myrold D. Comparative resis-tance and resilience of soil microbial communities and enzyme activities in adjacent native forest and agricul-tural soils. Microbial Ecology, 2009, 58: 414-424
[24] Fierer N, Jackson RB. The diversity and biogeography of soil bacterial communities. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103: 626-631
[25] 韩丽, 李立军, 赵举, 等. 内蒙古农牧交错带农田与草地土壤真菌群落特征差异性分析. 北方农业学报, 2022, 50(2): 38-46
[26] Li X, Sun ML, Zhang HH, et al. Use of mulberry-soybean intercropping in salt-alkali soil impacts the diversity of the soil bacterial community. Microbial Biotechnology, 2016, 9: 293-304
[27] 赵立君, 刘云根, 王妍, 等. 典型高原湖滨带底泥细菌群落结构及多样性特征. 微生物学通报, 2020, 47(2): 401-410
[28] Fazi S, Amalfitano S, Pernthaler J. Bacterial communities associated with benthic organic matter in headwater stream microhabitats. Environmental Microbiology, 2005, 7: 1633-1640
[29] Li BB, Roley SS, Duncan DS, et al. Long-term excess nitrogen fertilizer increases sensitivity of soil microbial community to seasonal change revealed by ecological network and metagenome analyses. Soil Biology and Biochemistry, 2021, 160: 108349
[30] Sun R, Chen Y, Han W, et al. Different contribution of species sorting and exogenous species immigration from manure to soil fungal diversity and community assemblage under long-term fertilization. Soil Biology and Biochemistry, 2020, 151: 108049
[31] Lidbury IDEA, Borsetto C, Murphy ARJ, et al. Niche-adaptation in plant-associated Bacteroidetes favours specialisation in organic phosphorus mineralisation. ISME Journal, 2021, 15: 1040-1055
[32] 吴高杰. 红树林沉积物有机碳的来源及其微生物碳同化研究. 博士论文. 厦门: 厦门大学, 2018
[33] 谭雪莲, 阚蕾, 张璐, 等. 城市森林土壤微生物群落结构季节变化. 生态学杂志, 2019, 38(11): 3306-3312
[34] Rousk J, Bååth E, Brookes PC, et al. Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME Journal, 2010, 4: 1340-1351
[35] 孙志朋, 郭玉冰, 刘建玲, 等. 长期有机肥磷肥配施对潮褐土有机质固存效应的影响. 河北农业大学学报, 2022, 45(2): 67-75
[36] Chen QL, Ding J, Zhu D, et al. Rare microbial taxa as the major drivers of ecosystem multifunctionality in long-term fertilized soils. Soil Biology and Biochemistry, 2019, 141: 107686