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Chinese Journal of Applied Ecology ›› 1995, Vol. 6 ›› Issue (4): 406-410.

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Bioassay of simazine in water and soil water solution using aquatic flowering plants as indicators

Ou Ziqing, Ren Liping, Jia Liangqing, Zhang Hairong, Chang Shijun, Sun Tieheng   

  1. Laboratory of of Ecological Process of Trace Substance in Terrestrial Ecosystem, Institute of Applied Ecology, Academia Sinica, Shenyang 110015
  • Received:1995-01-25 Revised:1995-06-23 Online:1995-10-25 Published:1995-10-25

Abstract: A simple, rapid and sensitiye bioassay using aquatic flowering plants as indicators is used for measuring available photosynthesis-inhibiting herbicides (simazine) in water and soil water solution.This method is based on the effect of simazine on oxygen release of photosynthesis, which can be directly measured using an oxygen electrode, and with which, the amount of simazine can be calculated.Measurement can be made within 10 min of treatment.This method is capable of measuring a minimum simazine concentration of 0.02mg·L-1.Ceratophyllum oryzetorum is a better indicator than Ranunculus trichophyllus and Alisma planlago-aquatica.The mean recoveries of simazine by bioassay using C.oryzetorum are 96 to 100%, which is comparable to those (98 to 100%) obtained by C18 column extraction and HPLC measurement.

Key words: Bioassay, Aquatic flowering plant, Simazine, Herbicide, Cd pollution, rice, alkaline fertilizer, Cd concentration of rice