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Isolation and determination of silverresistant bacteria plasmids

LI Junmin;JIN Zexin   

  1. Ecology Institute of Taizhou University,Linhai 317000,China
  • Received:2005-01-31 Revised:2005-06-19 Online:2006-02-18 Published:2006-02-18

Abstract: Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag+ solution,thirty bacteria strains with silver (Ag+)resistance were isolated,among which,the highest Ag+-resistant concentration was 80 mg·ml-1.The plasmids in these bacteria were extracted,with the detection rate of 76.67%.The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol·L-1 sodium benzoate,and 77.78% by 350 μg·ml-1 acridine orange.It was suggested that the Ag+ -resistance of bacteria was highly correlated with their plasmids.

Key words: Natural forest, Pinus densiflora, Individual biomass, Stand density