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Effects of different concentration ammonium-N on Hydrilla verticillata antioxidant enzymes under Cd stress

XU Qiu-jin; JIN Xiang-can; WANG Xing-min; HU Xue-wen; CHEN Shu-qin; YAN Chang-zhou   

  1. State Environmental Protection Key Laboratory for Lake Pollution Control, Research Center of
    Lake Eco-environment, Chinese Research Academy of Environmental Sciences, Beijing 100012,
    China
  • Received:2006-01-06 Revised:2006-11-30 Online:2007-02-10 Published:2007-02-10

Abstract: In this paper, submersed plant Hydrilla verticillata was cultivated in a 1/10 Hoagland culture medium added with different concentration NH4+-N (0, 0.5, 2.0, 4.0 mg·L-1) and 10 mg·L-1 cadmium to study the effects of NH4+-N on H. verticillata growth and its antioxidant en-zyme system under Cd stress, and approach the degradation mechanism of submersed macrophyte in eutrophic and polluted water bodies. The results showed that under 10 mg·L-1 Cd stress, the chlorophyll content of H. verticillata decreased obviously, and SOD and POD activities increased first but declined then. NH4+-N accelerated the stress effect of Cd. Under 0.5 h joint stress of Cd and NH4+-N, SOD activity increased significantly, while such an increase needed 3 hours under single Cd stress. Relative to chlorophyll and protein, antioxidant enzyme system was an early sensi-tive index, and SOD was more sensitive than POD. After two days exposure to Cd plus NH4+-N, H. verticillata was mainly stressed by Cd, and NH4+-N stress could be neglected.

Key words: Global consequences, Prevention and control strategies, Ecosystem, Biodiversity, Risk analysis