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Chinese Journal of Applied Ecology ›› 2012, Vol. 23 ›› Issue (02): 511-518.

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Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops.

XU Ying-ying1, DU Bing-hai1,2, YAO Liang-tong1, JIN Feng-li1, WANG Cui-cui1, WANG Xuan1, DING Yan-qin1   

  1. 1Department of Microbiology, College of Life Science, Shandong Agricultural University, Tai’an 271018, Shandong, China; 2 Shandong Province Key Laboratory for Agriculture Microbiology,  Tai’an 271018, Shandong, China
  • Online:2012-02-18 Published:2012-02-18

Abstract: By adopting antimicrobial spectrum test, BOXAIR-PCR, physiological and biochemical, and 16S rDNA sequencing analysis, this paper analyzed the diversity of 55 antagonistic bacterial strains isolated from the  rhizosphere  of 10 cash crops. There was a high diversity of the antagonism of the strains. Based on BOXAIR-PCR, all the strains were clustered into 7 groups at the similarity level of 721%, and divided into 25 groups at the similarity level of 850%. All the strains belonged to Bacillus, Paenibacillus, Brevibacillus, Pseudomonas and Alcaligenes, respectively. The antagonistic bacteria isolated from the rhizosphere had high genetic diversity and high diversity in antagonistic activity.

Key words: rhizosphere, antimicrobial spectrum, BOXAIR-PCR, 16S rDNA, diversity