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Chinese Journal of Applied Ecology ›› 2023, Vol. 34 ›› Issue (6): 1500-1508.doi: 10.13287/j.1001-9332.202306.012

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Responses of soil bacterial and fungal communities to altered precipitation in a desert steppe

GUO Rong1,2, WU Xudong3, WANG Zhanjun3, JIANG Qi3, YU Hongqian3, HE Jing1,2, LIU Wenjuan1,2, MA Kun1,2*   

  1. 1Cultivation Base of State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China;
    2Ministry of Education Key Laboratory for Restoration and Reconstruction of Degraded Ecosystems in Northwest China, Ningxia University, Yinchuan 750021, China;
    3Institute of Forest and Grassland Ecology, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021, China
  • Received:2022-10-27 Accepted:2023-04-11 Online:2023-06-15 Published:2023-12-15

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Abstract: To investigate the response mechanisms of soil bacterial and fungal communities to the changes of preci-pitation in a desert steppe of Ningxia, we conducted a three-year precipitation control experiment following completely randomized design. There were five treatments, natural precipitation (T0), 50% less in precipitation (T1), 25% less in precipitation (T2), 25% more in precipitation (T3) and 50% more in precipitation (T4). By using Illumina high-throughput sequencing and bioinformatics analysis, we investigated the effects of increased and decreased precipitation on soil bacterial and fungal communities, and examined the correlations between soil physicochemical properties, plant communities and soil bacterial and fungal communities. The result showed that the richness of soil bacteria and fungi was highest in the T4 treatment. In addition, the relative abundance of Chloroflexi, the predominant phyla of soil bacteria was more sensitive to precipitation change. However, the relative abundance of only Ascomycota, a rare fungal taxon, responded to precipitation changes. Results of redundancy analysis showed that the first two axes accounted for 92.8% and 87.4% of the total variance for soil bacterial and fungal community composition, respectively. Precipitation and soil pH were the most important environmental factors driving changes in soil bacterial diversity and community composition. On the one hand, precipitation had a direct positive effect on bacterial diversity and community composition. On the other hand, precipitation changed pH by affecting soil moisture, which in turn had a significant indirect effect on bacterial diversity and community composition. Plant community biomass, plant species richness, and soil pH were the most influential environmental factors affecting fungal diversity and community composition. Precipitation had no direct effect on soil fungal community, but had a significant indirect effect by changing plant community richness and soil pH. The response mechanisms of bacterial and fungal communities in soil differed significantly under different precipitation regimes in the desert grasslands of Ningxia.

Key words: precipitation variation, bacterial community, fungal community, richness, diversity, high-throughput sequencing, structural equation modeling