Analysis and identification of Liriomyza sativae-attractants from cowpea and kidney bean volatiles

Abstract

Abstract: In this paper,the volatiles from cowpea and kidney bean,the main host plants of American leafminer Liriomyza sativae,were collected and extracted by a self-designed device and solid phase microextraction (SPME) technique,and analyzed by GC-MS.The results showed that the volatiles from the two beans had the same components mainly consisted of 2-hexenal,3-Hexen-1-ol,2-Hexen-1-ol,1-Octen-3-ol,3-Hexenol acetate,α-Ionone and β-Ionone.The lure made of the seven components was attractive to L.sativae in field trapping trials.Detailed analyses indicated that α-Ionone and β-Ionone might play important roles in the searching behavior of L.sativae for host plants.

Key words: Solid phase microextraction, Liriomyza sativae, Volatile organic compounds, Field trapping trials

CLC Number:

Q968.1

References

[1] Affeldt HA, Thimijan RW, Smith FF, et al. 1983. Response of the greenhouse whitefly (Homoptera:Aleyrodidae) and the vegetable leafminer (Diptera:Agromyzidae) to photospectra. J Econ Ent, 76:1405～1409
[2] Bicchi C, Drigo S, Rubiolo P. 2000. Influence of fibre coating in headspace solid-phase microextraction - Gas chromatographic analysis of aromatic and medicinal plants. J Chromatogr A, 892:469485
[3] Buchbauer G, Jirovetz L, Wasicky M. 1994. Comparative investigation of Douglas fir headspace samples, essential oils, and extracts(needles and twigs) using GC-FID and GC-FTIR-MS. J Agric Food Chem, 42:2852 ～2854
[4] Chen W-L(陈文龙).2001. Mechanisms of ost selection and location of vegetable leafminer, Liriomyza sativae Blanchard (Diptera:Agromyzidae). Ph. D. Dissertation. Shanghai:Shanghai Institute of Entomology, Chinese Academy of Sciences. 55～60(in Chinese)
[5] Chen Y(陈艳). 1998. Spatial pattern and vertical distribution of vegetable leaf miner Liriomyza sativae Blanchard (Diptera:Agromyzidae). J Fujian Agric Univ(福建农业大学学报), 27(3):257 ～260 (in Chinese)
[6] Dai X-H(戴小华), You M-S(尤民生), Fu L-J(付丽君). 2001.The influences of water-soluble substances from other plants on the host-selection of Liriomyza sativae. J Fujian Agric Univ (福建农业大学学报), 30 (4) :490～492(in Chinese)
[7] Delton AV, Paul J, David JB. 2000. Solid phase micro-extraction for the determination of volatile organics in the foliage of fraser fir (Abies fraseri). Microchem J, 65:269 ～276
[8] Deeming JC. 1992. Liriomyza sativae Blanchard (Diptera:Agromyidae) established in the Old World. Trop Pest Man, 38:218～219
[9] Deng J-Y(邓建宇).2002. Studies on insect sex pheromone formulations and effects of plant volatiles on attractiveness of sex pheromones. Ph. D. Dissertation. Shanghai:Shanghai Institute of Plant Physiology & Ecology, Chinese Academy of Sciences. 32～37(in Chinese)
[10] Deng W-X(邓望喜), Wang Z-X(汪忠信), Peng F-Q(彭发青).1999. Studies on host plant selectivity of Liriomyza sativae to vegetable varietyes of Leguminosae and Cucurbilaceae. J Huazhong Agric Univ(华中农业大学学报), 18(4):317～320(in Chinese)
[11] Dicke M, Minkenberg OPMJ. 1991. Role of volatile chemicals in foraging behavior of the leafminer parasitoid Dacnusa sibirica(Diptera:Agromyzidae). J Insect Behav, 4(4) :489 ～500
[12] Du J-W(杜家纬). 1988. Insect Pheromone and Its Application.Beijing:China Forestry Press. (in Chinese)
[13] Du J-W (杜家纬). 2001. Plants-insects chemical communication and their behavior control. Acta Phytophysiol Sin (植物生理学报), 27(3):193～200(in Chinese)
[14] Fabio A, Luiz A. 2002. Applications of solid-phase microextraction to chemical analysis of live biological samples. Trends Anal Chem,21(6 + 7) :428～438
[15] Fagoonee I, Toory V. 1983. Preliminary investigations of host selection mechanisms by the leafminer Liriomyza trifolii. Insect Sci Appl, 4(4) :337～341
[16] Heath RR, Manukian A. 1992. Development and evaluation of systems to collect volatile semiochemicals from insects and plants using a charcoal-infused medium for air purification. J Chem Ecol, 18(7):1209～1226
[17] HuangJ-H(黄京华), Li H-S(黎华寿), Yang J(杨军).2004.Chemical componets of Vetiveria zizanioides volatiles. Chin J Appl Ecol(应用生态学报), 15(1):170～172(in Chinese)
[18] Johnson MW, Oatman ER, Wyman JA. 1980. A technique for monitoring Liriomyza sativae in fresh market tomatoes. J Econ Ent, 73(4) :552～555
[19] Johnson MW, Welter SC, Toscano NC. 1983. Reduction of tomato leaflet photosynthesis rate by mining activity of Liriomyza sativae(Diptera:Agromyzidae). J Econ Ent, 76(5):1061 ～1063
[20] Ling B (凌冰), Zhang M-X(张茂新), Kong C-H(孔垂华), et al. 2003. Chemical composition of volatile oil from Chromolaena odorata and its effect on plant, fungi and insect growth. Chin J Appl Ecol(应用生态学报), 14(5) :744～766(in Chinese)
[21] Minkenberg OP, Ottenheim JJ. 1990. Effect of leaf nitrogen content of tomato plants on preference and performance of a leafmining fly.Oecology, 83(3) :291 ～298
[22] Pare PW, Tumlinsom JH. 1999. Plant volatiles as a defense against insect herbivores. Plant Physil, 121:325～331
[23] Servili M, Roberto S, Taticchi A. 2000. Relationships between the volatile compounds evaluated by solid phase microextraction and the thermal treatment of tomato juice:Optimization of the blanching parameters. Food Chem, 71:407～415
[24] Spencer KA. 1973. Agromyzidae (Diptera) of economic importance. Ser Ent, 9:410～418
[25] Wei H-Y(魏洪义), Du J-W(杜家纬). 2003. Identification cf active components of sex pheromone for Sidemia depravata and field trapping. Chin J Appl Ecol (应用生态学学报), 14(5):730 ～732(in Chinese)
[26] XianJ-D(冼继东),Chen R(陈荣),Yang Q-J(杨全杰), et al.1998. Study on biology and control of Liriomyza sativae Blandchard in Hainan island (Diptera:Agromyzidae). J Hainan Univ(Nat Sci) (海南大学学报·自然科学版), 16(3):247 ～250(in Chinese)
[27] Xian J-D(冼继东),Liang G-W(梁广文),Zeng L(曾玲).2003. Suppressive effect of plant secondary substances and ecological measures on Liriomyza sativae population. Chin J Appl Ecol(应用生态学报), 14(11):1943～1946(in Chinese)
[28] XianJ-D(冼继东),Pang X-F(庞雄飞),Zeng L(曾玲).2003.Suppressive effect of secondary substances on Liriomyza sativae population. Chin J Appl Ecol (应用生态学报), 14 (1):97 ～100 (in Chinese)
[29] Yuan Z-F(袁志发), Zhou J-Y(周静芋). 2000. Experiments Design and Analysis. Beijing:Higher Education Press. 292 ～303 (in Chinese)
[30] Zhao Y-X(赵云鲜), Kang L(康乐). 2001. Host plant preference of the polyphagous leafminers Liriomyza spp. Acta Ent Sin (昆虫学报), 44(4) :567～573(in Chinese)
[31] Zoltan M, Ralph S. 2001. Solid phase microextraction as a tool for trace element speciation. Spectrochim Acta Part B, 56:233 ～260