Screening of a phenanthrene-degrading bacterium and its degradation conditions

Abstract

Abstract: Several PAHs-degrading bacteria were isolated from the soil near a petrochemicals factory,and one strain F10a identified as B.sphaericus was chosen for use.The study on the phenanthrene-degradation potential of the strain and its affecting factors showed that at 28 ℃,the degradation rate of phenanthrene (50 mg·L^-1) was 98.12% after 27 hours rotary culture,and 98.47% after 84 hours static culture.F10a had a good phenanthrene-degradation capability when the pH was 4,6 and 8,but its growth was inhibited when pH was 10.Cr²⁺ was toxic to the strain,Cu²⁺ could delay the degradation of phenanthrene,while Zn²⁺ and Pb²⁺ had no significant effects.The degradation rate of phenanthrene (200 mg·L^-1) was 99.6% after 84 hours rotary culture.A significant positive relationship was found between bacterial growth and phenanthrene degradation.

Key words: Degradation, Phenanthrene, Polycyclic aromatic hydrocarbons, Bsphaericus

CLC Number:

Q93
X172

ZHOU Le, SHENG Xiafang, ZHANG Shijin, LIU Jing . Screening of a phenanthrene-degrading bacterium and its degradation conditions[J]. Chinese Journal of Applied Ecology, 2005, 16(12): 2399-2402.

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