Screening of phytase-producing strain and its optimal solid state phytase-producing conditions

Abstract

Abstract: Phytase can catalyze phytate hydrolyzed into inositol and phosphoric acid,and its application could make the phytic acid in animal feeds become more available,decrease feed cost,and at the same time,protect eco-environment.In this paper,a phytase-producing strain Trichoderma Viride LH374 was obtained through isolation and mutation,and its optimal conditions for solid state fermentation and phytase production were investigated.The results suggested that the optimal conditions of solid state fermentation were straw:rice bran ratio 8:2,medium initial pH 6.5,temperature 30 ℃,culture duration 96 h,and moisture content 60%.Under the optimal conditions,LH374 could accumulate phytase up to 1 580 U·g^-1.

Key words: Phytase, Strain screening, Solid state fermentation, Optimal conditions of phytase-producing

CLC Number:

S154.39

WANG Shihua, HU Kaihui, LIN Wenxiong . Screening of phytase-producing strain and its optimal solid state phytase-producing conditions[J]. Chinese Journal of Applied Ecology, 2005, 16(11): 2154-2157.

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