Accumulation of La(NO3)3 in mice liver and its genetic toxicity

Abstract

Abstract: By using ICP-MS method,this paper determined the accumulation of La in mice liver after the mice being fed with La(NO₃)₃ water solution for thirty days,and through in vitro experiment,studied the effect of La(NO₃)₃ on the micronucleus rate of mice bone marrow cells and the cleavage action of La(NO₃)₃ on genome DNA to investigate the genetic toxicity of La.The results showed that when the treated concentration was 1 000,500,300 and 50 μg·ml^-1,the amount of La in mice liver reached 1.46,0.558,0.529 and 0.083 μg·g^-1,respectively.Compared with control,the La amount in disposed groups' mice livers increased with increasing La(NO₃)₃ concentration in water(r=0.980).T-test results showed that there existed significant differences in 1 000,500 and 300 μg·ml^-1 disposed groups when compared with the control(P<0.05).The micronucleus rate of mice bone marrow cells increased with increasing La(NO₃)₃ concentration in water(r=0.853).The in vitro experiments showed that La(NO₃)₃ could make DNA cleaved.It could be concluded that that La might be accumulated in organisms,and could induce the damage of genetic material in cells.

Key words: La(NO3)3, ICP-MS, Micronucleus rate, DNA cleavage, Genetic toxicity

CLC Number:

Q784

WANG Yang, NIE Liuwang, CHEN Wen, ZHANG Jifeng, CHEN Qilong . Accumulation of La(NO₃)₃ in mice liver and its genetic toxicity [J]. Chinese Journal of Applied Ecology, 2005, 16(1): 133-136.

References

[1] Cao J(曹焦), Lin Z(林真). 2000. Micronucleus Test Beijing:Military Medical Science Press. 42～61(in Chinese)
[2] Cao X-D(曹心德), Zhao G-W(赵贵文), Zha L-X(查立新), et al.1998. A method for determination of soluble rare earth elements in soils by inductively coupled plasma-atomic emission spectrometry.Environ Sci(环境科学), 19(2):66～70(in Chinese)
[3] Chen J-Y (陈祖义). 1999. Reflection on the biological effects of rare earth and the cumulative impacts of its agricultural application.Rural Eco-Environ (农村生态环境), 15(3):44～48(in Chinese)
[4] DingS-M(丁士明),ZhangZ-L(张自立),LiangT(梁涛),et al. 2003. Effects of extraneous rare earths(REs) on form of soil exchangeable REs and heavy metals. Environ Sci (环境科学), 24(4):122～126(in Chinese)
[5] DongZ-Y(董智勇),ChenD(陈东),LiuY(刘颖),etal.2003. Effects of mixed rare earth change on calcium content and it's deposition in the liver of pregnant and fetal rats. J Jilin Univ (吉林大学学报), 29(1):13～15 (in Chinese)
[6] Fan G-Q(范广勤), Liu Z-G(刘志刚), Zheng H-L(郑辉), et al. 2002. Study on effects of exposure to rare earth elements on function of immune system of children. Chin J Public Health (中国公共卫生), 18(8):952～953(in Chinese)
[7] GaoJ-H(高晋华),Wang Q(王青),Wang A-F(王爱凤), et al.1998. A Study on La-rich rare-earth' s effects on salt-resistance of wheat seedling. J Shanxi Agric Univ (山西农业大学学报), 18(2):115～117(in Chinese)
[8] HuX(胡忻),Dai L-M(戴乐美),Wang X-R(王晓蓉), et al.2002. The relationship between accumulation of lanthanum on wheat seedlings and the seedlings growth. China Environ Sci (中国环境科学), 22(2):137～141 (in Chinese)
[9] Hua R-M(花日茂), Zhang Z-L(张自立), Wu X-A(吴新宏), et al. 2003. Effects of rare earth elements on amino acid contents in earthworms. Chin J Appl Ecol (应用生态学报), 14(10):1787～1789(in Chinese)
[10] Ju T-J(琚彤军),Liu P-L(刘普灵), Shi H(石辉).2002. Study on REE absorption and distribution characteristics in crops. J Chin RareEarth Soc (中国稀土学报), 20 (spec. issue):163～167 (in Chinese)
[11] Komiyama M, Matsumura K, Matsumoto Y. 1992. Unprecedentedly fast hydrolysis of the RNA dinucleoside monophosphates ApA and UpU by rare earth metal ions. J Chem Soc Chem Comm, 8:640～641
[12] Komiyama M, Shiiba T, Kodama T, et al. 1994. DNA hydrolysis by cerium(Ⅳ) does not involve either molecular-oxygen or hydrogenperoxide. Chem Let, 6:1025～1028
[13] Liang T(梁涛),Wang L-J(王立军),Zhang S(章申), et al.2001. Accumulation of lanthanum with various species in Oryzias latipes fish body. J Chin Rare Earth Soc(中国稀土学报), 19(5):443～446 (in Chinese)
[14] Liu S-J(刘书娟), Wang L-J(王立军), Zhang S(章申), et al.1997. Effect of long-term foliage-dressing rare earth elements (REEs) on their distribution, accumulation and translocation in soilspring wheat system. Ch in J Appl Ecol (应用生态学报), 8 (1):55～58 (in Chinese)
[15] Liu X-Y(刘晓勇),Rong X-H(荣先恒), Hu S-C(胡泗才), et al.2000. Effects of cerium on lift of fruit fly and silk worm and on weight of cocoon. J Nanchang Univ(南昌大学学报), 24(2):192～194(in Chinese)
[16] Liu Z-H(刘志宏),Lei Z-M(雷志明),Wei X-T(魏雪涛), et al.2002.The effects of exposure to rare earth(NO3)3 on the immune function of mice off spring via milk. Chin J Prev Med (中华预防医学杂志), 36(2):394～397(in Chinese)
[17] Lu R(卢然), Ni J-Z(倪嘉缵). 2002. Mechanism of rare earth effect on liver. J Chin Rare Earth Soc (中国稀土学报), 20(3):193～198(in Chinese)
[18] Meng Z-Q(孟紫强), Ruan A-D(阮爱东),Zhang B(张波), et al. 2002. Micronuclei induced by sulfur dioxide inhalation in bone marrow cells of mice. J Environ Health (环境与健康杂志), 19(4):298～299 (in Chinese)
[19] Ni J-Z(倪嘉缵). 1995. Bioinorganic Chemistry of Rare Earth Elements. Beijing:Science Press. 13～38 (in Chinese)
[20] Sambrook J, Frisch EF, Maniatis T. 1989. Molecular Cloning, A Laboratory Manual. New York:Cold Spring Harbor Laboratory Press. 78～120
[21] Takasaki B, Chin J. 1994. Cleavage of phosphate diester backbone of DNA with cerium and molecular oxygen. J Amer Chem Soc,116:1121～1122
[22] TangX-Y(唐欣昀),Zhang Z-L(张自立),Cheng Y(程勇),et al. 1997. Effect of Ce accumulation on soil microflora in yellow cinnamon soil. Chin J Appl Ecol (应用生态学报), 8(6):585～588(in Chinese)
[23] Wang J-F(汪建飞),He Y-Z(何友昭),Cao X-D(曹德). 1999.Effects of rare earth micro-fertilizer on hot pepper quality and determination of RE elements in hot pepper using ICP-MS method.Chin Rare Earths(稀土), 20(3):48～50(in Chinese)
[24] Wang M-Q(王敏奇), Xu Z-R(许梓荣). 2003. Effect of supplemental lanthanum on growth performance of pigs and its security as a feed additive. Chin J Vet Sci (中国兽医学报), 23(1):88～90(in Chinese)
[25] Wei Z-G(魏正贵),Wan S-K(宛寿康),Zhang X(张巽), et al.2001. ICP-AES studies on characteristics of distribution, accumulation and transportation of rare earth elements in soil-Dicranopteris dichotoma system. Chin J Appl Ecol (应用生态学报), 12(6):863～866 (in Chinese)
[26] WeiZ-G(魏正贵),Yin M(尹明),ZhangX(张巽),et al.2001. Distribution, accumulation and transportation of rare earth elements in soil-dicranoprteris linearis system at South Jiangxi. Acta Ecol Sin (生态学报), 21(6):900～906(in Chinese)
[27] Xu X-K(徐星凯), Wang Z-J(王子健), Liu Y(刘琰), et al.2001. Influences of rare earths on the hydrolysis of urea and its hydrolyzed product in soil. Chin J Appl Ecol (应用生态学报), 12(5):739～742 (in Chinese)
[28] Xu Z-G(徐子刚),Shang W(商玮),Shen Y-Z(沈雅珍), et al.2000. Analysis of trace elements in human hair, human serum and cancer by ICP-MS.J Zhejiang Univ (Eng Sci) (浙江大学学报·工学版), 34 (5):479～483 (in Chinese)
[29] Yuan Y-J(元英进), Hu G-W(胡国武), Wang C-G(王传贵), et al. 1998. Effect of rare earth compounds on the growths, taxol biosynthesis and release of taxus cuspidate cell culture. J Chin Rare Earth Soc(中国稀土学报), 16(1):56～60(in Chinese)
[30] YuanZ-K(袁兆康),LiuY(刘勇),YuH-Q(俞慧强),etal.2003.Study on relationship between rare earth level in blood and health condition of residents. Chin J Public Health (中国公共卫生), 19(2):133～135(in Chinese)
[31] ZhangJ(章健),ChengH-Y(承河元),Gao Q(高倩),etal.2000. Effect of lanthanum on growth and biochemical property of Sclerotinia sclerotiorum. Chin J Appl Ecol (应用生态学报), 11(3):382～384 (in Chinese)
[32] Zhang W-P(张万萍), Yang M (杨民), Mo X-Q(莫小青), et al. 2003. Effects of soaking with rare earth elements on seeds germination and seedings growth of cucumber. Seeds (种子), (1):28～30 (in Chinese)

Accumulation of La(NO₃)₃ in mice liver and its genetic toxicity

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	HU Shen, WANG Xiao-chun, YANG Jin-yan. Interannual variation patterns of heavy metals concentrations in tree rings of Larix gmelinii near Xilin Lead-zinc Mine, Yichun of Northeast China. [J]. Chinese Journal of Applied Ecology, 2013, 24(6): 1536-1544.
[2]	WANG Yang,NIE Liuwang,CHEN Wen,ZHANG Jifeng,CHEN Qilong . Accumulation of La(NO₃)₃ in mice liver and its genetic toxicity [J]. Chinese Journal of Applied Ecology, 2005, 16(01): 133-136 .