Secondary compounds in rice varieties resistant to Nilaparvata lugens

Abstract

Abstract: By the method of high-performance liquid chromatography (HPLC),this paper studied the contents of 13 secondary compounds from 130 samples of 26 rice varieties resistant to brown planthopper (BPH) Nilaparvata lugens biotype Ⅱ.A prediction model was established through principal component analysis and multiple regression analysis: Y=3.4593-0.02491X₁+0.08475X₂-0.04227X₈+0.1174X₁₂.The relationships between the BPH-resistant level of rice varieties and the area values of chromatographic peaks were significant (r²=0.84,P<0.01).The results demonstrated that Peak 1,Peak 2,Peak 8 and Peak 12 were the major secondary compounds affecting the resistance to BPH biotype Ⅱ,which suggested that the BPH resistance of the varieties came from the action of several secondary compounds that varied in contribution to the resistance.

Key words: Resistant secondary compounds, Rice, Resistant breeding to insects, HPLC, Brown planthopper

CLC Number:

Q946.8

ZHAO Ying, HUANG Fengkuan, TONG Xiaoli, LING Bing, PANG Xiongfei. Secondary compounds in rice varieties resistant to Nilaparvata lugens[J]. Chinese Journal of Applied Ecology, 2004, (11): 2161-2164.

References

[1] Besson E,Dellamonica G,Chopin J,et al.1985.C-gycosyl flavone from Oryza sativa.Phytochemistry,24(5):1061～1064
[2] Chung IM,Kim KH,Ahn JK,et al.2002.Screening of allelochemicals on barnyargrass (Echinochloa crus-galli) and identification of potentially allelopathic compounds from rice (Oryza sativa) variety hull extracts.Crop Prot,21:913～920
[3] Cohen MB,Alam SN,Medina EB,et al.1997.Brown planthopper,Nilaparvata lugens,resistance in rice cultivar IR64: Mechanism and role in successful N.lugens management in Central Luzon,Philippines.Ent Exp Appl,85:221～229
[4] Hu G-W(胡国文),Liang T-X(梁天锡),Liu G-J(刘光杰),et al.1994.The extraction,chemical analysis and bioassays of secondary volatiles from rice varieties susceptible and resistant to the whitebacked planthopper,Sogatella furcifera(Horváth)(Homoptera:Delphacidae).Chin J Rice Sci(中国水稻科学),8(4):223～230(in Chinese)
[5] Kim M,Koh HS,Fukami H.1985.Isolation of C-glycosylflavones as the probing stimulant of planthoppers in rice plant.J Chem Ecol,11:441～452
[6] Kong C-H(孔垂华).2002.Frontier fields of plant chemical ecology in the 21 st century.Chin J Appl Ecol(应用生态学报),13(3): 349～353(in Chinese)
[7] Kong C-H(孔垂华),Xu X-H(徐效华),Hu F(胡飞),et al.2002.Using specific secondary metabolites as markers to evaluate allelopathic potentials of rice varieties and individual plants.Chin Sci Bull(科学通报),47(3):203～206(in Chinese)
[8] Li R-B(李容柏),Qin X-Y(秦学毅).1994.The main findings of resistant identification of Guangxi wild rice to diseases and pests.Guangxi Sci(广西科学),1(1):83～85(in Chinese)
[9] Li R-D(李汝铎),Ding J-H(丁锦华),Hu G-W(胡国文),et al.1996.The Brown Planthopper and Its Population Management.Shanghai:Fudan University Press.72～76(in Chinese)
[10] Ling B(凌冰),Zhang M-X(张茂新),Kong C-H(孔垂华),et al.2003.Chemical composition of volatile oil from Chromolaena odorata and its effect on plant,fungi and insect growth.Chin J Appl Ecol(应用生态学报),14(5):744～746(in Chinese)
[11] Liu G-J(刘光杰),Zheng Y-C(郑宜才),Gui L-Q(桂丽琴),et al.1999.On the screening methods for resistance to rice planthoppers (Homoptera:Delphacidae) in some Chinese rice varieties.Acta Agric Zhejiangensis(浙江农业学报),11(6):306～310(in Chinese)
[12] Lü Z-X(吕仲贤),Yü X-P(俞晓平),Tao L-Y(陶林勇),et al.2002.Resistance evaluation of newly-bred rice varieties (lines) to brown planthopper Nilaparvata lugens Stl in China.Sci Agric Sin(中国农业科学),35(2):225～229(in Chinese)
[13] Mattice JD,Dilday RH,Gbur EE,et al.2001.Barnyardgrass growth inhibition with rice using high-performance liquid chromatography to identify rice accession activity.Agron J,93:8～11
[14] Maxwell FG,Jennings PR.1980.Breeding Plants Resistant to Insects.New York:John Wiley & Sons.23～61
[15] Niemeyer HM.1988.Hydroxamic acids (4-hydroxy-1,4-benzoxazin-3-ones),defense chemicals in the Gramineae.Phytochemistry,27(11):3349～3358
[16] Niemeyer HM,Perez FJ.1995.Potential of hydroxamic acids in the control of cereal pests,diseases,and weeds.ACS Symp Series,582:260～270
[17] Pang X-F(庞雄飞).1999.Plant protectants and plant immune engineering against insect pests.J World Sci-Technol Res Dev(世界科技研究与发展),21(2):24～28(in Chinese)
[18] Qin J-D(钦俊德).1987.The Relationship of Plants and Insects.Beijing: Science Press.38～58 (in Chinese)
[19] Wei S-M(韦素美),Luo S-Y(罗善昱),Huang F-K(黄凤宽),et al.1998.Resistance evaluation of newly-bred rice varieties in Guangxi to different biotypes of brown planthopper Nilaparvata lugens (Stl).J Guangxi Agric Sci(广西农业科学),(5):238～239(in Chinese)
[20] Yan S-C(严善春),Zhang D-D(张丹丹),Chi D-F(迟德富).2003.Advances of studies on the effects of plant volatiles on insect behavior.Chin J Appl Eco(应用生态学报),14(2):310～313(in Chinese)
[21] Zhang G,Zhang W,Lian B,et al.1999.Insecticidal effects of extracts from two rice varieties to brown planthopper,Nilaparvata lugens.J Chem Ecol,25(8):1843～1853
[22] Zhou M-Z(周明(羊)).1992.Principles and Applications of Crop Resistance to Insects.Beijing:Beijing Agricultural University Press.37～41(in Chinese)