Effect of fertilization on soil enzymes and microbes in Larix gemlinii plantations

Abstract

Abstract: One year-old second generation stand and 14 year-and 34 year-old first generation stands in larch plantations were studied at the Maoershan Mt. Experimental Forest Farm. The forestland of these three stands was treated with various fertilizations. The results showed that fertilization could promote or restrain soil enzyme activity and microbial amount with different degrees, and its effect on soil physiological activity was more apparent. The effect of the same fertilisation treatmenu on soil enzyme activity and microbial amount was different at different development stages of the stands. For one year-old stand, the best fertilization scheme was treatment 9, its soil enzyme activities (catalase, proteinase, polyphenoloxidase,urease，and saccharase), total microbial amount, and amount of bacteria, actinomyces and fungus being increased by 413.49%,22.10%，20. 56%，220. 00%，49. 46 %，238. 88 %，247. 24 %,106. 70 %，and 366 . 67 %,respectively, as compared with the mntrol. For 34 year-old stand, the best fertilization scheme was treatment 5, its soil enzyme activities(catalase, proteinase,polyphenoloxidase,urease，and saccharase), total microbial amount, and amount of bacteria and fungus was increased by 30. 44 %，16.91%,0.22%,43.06%，124.18 %，119. 92 %, 87 . 66 %, and 17. 57&，respectively in rhizosphere soil, and by 24 . 55 %，77. 0l %，168.62 %，251. 85 %，183. 33 %, 250. 0 %，38 . 24 % and 128 . 57 %，respectively in non-rhizosphere soil. For 14 year-old young stand, it needed a proper amount of nitrogen fertilizer and organic-mineral fertilizers, and the better fertilisation schemes were treatments 2 and 9. The soil enzyme activities (catslose, proteinase, and，urease)in treatment 2 was increased by 44. 39 %, 94 . 83 %，and 4 . 62 %，respectively in rhizcsphere soil, and by 13 . 98 %，10. 70 % and 129 . 76 %，respectively in non-rhizosphere soil. Total soil microbial amount and the amount of bacteria and fungus in treatment 9 was increased by 176. 49 %, 266. 63&, and 198. 04 %, respectively in rhizcsphere Broil, and by 275 . 56 %, 66. 67 % and 143 . 75 %，respectively in non-rhizasphere soil.

Key words: Laris gmelinii plantation, Fertilization, Enzyme activity, Microbial amount

CLC Number:

S714

CHEN Lixin. Effect of fertilization on soil enzymes and microbes in Larix gemlinii plantations[J]. Chinese Journal of Applied Ecology, 2004, (6): 1000-1004.

References

[1] Chen L-X(陈立新),Chen X-W(陈祥伟),Duan W-B(段文标).1998.Larchlitter and soil fertility.Chin J Appl Ecol(应用生态学报),9(6):581 586(in Chinese)
[2] Chen L-X(陈立新),Chen X-W(陈祥伟),Shi G-X(史桂香).1998.Study on improving forestland quality of larch plantation.J Northeast For Univ(东北林业大学学报),26(1):6～11(in Chinese)
[3] Chen YL, Han SJ, Shi XM. 2001. Nutrient characteristics in rhizosphere of pure and mixed plantations of Manchurian walnut and Dahurian larch. J For Res, 12(1): 18～ 20
[4] Akai Qguna(赤井童男), Asada Fushio(浅田节夫). 1962. Study on bad forestland of larch plantations at the second generation (the eighth report). Congress Reports Japan Soc For (日林讲集), 72:145 ～ 148 (in Japanese)
[5] Gao Y-X(高雅贤). 1983. Study on soil water and fertility dynamics in larch plantations. For Sci Tech (林业科技), (2): 9 ～ 13 (in Chinese)
[6] Guan S-Y(关松荫), et al. 1986. Soil Enzyme and Its Research Method. Beijing: Agriculture Press. (in Chinese)
[7] Microbe Section of Nanjing Soil Institute of CAS. 1985. Research Method About Soil Microbe. Beijing: Agriculture Press. (in Chinese)
[8] Asada Fushio(浅田节夫). 1981.Silviculture on Larch Plantations.Tokyo: Agro-forestry Publication Company. 162 ～ 168 (in Japanese)
[9] Sun C-L(孙翠玲), GuoY-W(郭玉文), Guo Q-S(郭泉水). 1997.The change of nutrient contents and its effect on forest growth on the soil degraded by successive crop of Japanese larch. For Res (林业科学研究), 10(3) :321 ～ 324(in Chinese)
[10] Wei M-R(卫茂荣), Zhang X-H(张新华), Xu A-J(徐爱军).1994. Seedling nutrition diagnosis of Japanese larch by integrated method of fertilization diagnosis. Chin J Soil Sci (土壤通报), 25(5) :227～229(in Chinese)
[11] Xu G-H(许光辉). 1986. Handbook of Analysis Method About Soil Microbe. Beijing: Agriculture Press. (in Chinese)
[12] Yan D-R(阎德仁),Wang J-Y(王晶莹), Yang M-R(杨茂仁).1997. Tendency of soil degradation in the pure larch plantations.Chin J Ecol (生态学杂志), 16(2) :62～66(in Chinese)
[13] Yan X-S(严昶升). 1988. Soil Fertility and Its Research Method.Beijing: Agricultural Press. (in Chinese)
[14] Zhang H-L(张慧亮), Gao Y-X(高雅贤), Xiang H-H(向黄怀).1987. Soil fertility status in larch plantations. In: Forestry Research Institute, Academy of Forestry of Heilongjiang Province, eds. Proceedings of Science and Technology of Forest Management. Beijing:China Forestry Press. 144～ 147(in Chinese)
[15] Zhang Y-D(张彦东), Yu X-J(于兴君), Yang W-H(杨文化).1997. Nutrient characteristics in forestland and rhizosphere of mixed plantations of Manchurian walnut and Dahurian larch. In:Shen G-F(沈国航), Zhai M-P(翟明普), eds. Mixed Plantation Research. Beijing: China Forestry Press. 145～ 149(in Chinese)
[16] Zhang Y-D (张彦东东), Wang Z-Q(王政权), Wang J (王晶).1997. Soil enzyme activeness of pure and mixed plantations of Manchurian walnut and Dahurian larch. In: Shen G-F (沈国舫), Zhai M-P(翟明普), eds. Mixed Plantation Research. Beijing:China Forestry Press. 141 ～ 144 (in Chinese)
[17] Zbou L-K (周礼恺) ed. 1987. Soil Enzymology. Beijing: Science Press. (in Chinese)
[18] Zhou X-M(邹晓明). 1992. Effect of tree biogeochemistry on soil fertility. In: Liu J-G(刘建国) ed. Great Ecology Theory at Present Generation. Beijing: China Science and Technology Press. 65 ～ 72(in Chinese)