Effects of different fertilization treatments on soil microbial functional diversity of dry tableland wheat field in south Shanxi Province.

doi:10.13287/j.1001-9332.202303.014

Abstract

Abstract: Understanding the effects of different fertilization treatments on microbial functional diversity in loess tableland wheat soil in south Shanxi Province can provide the theoretical basis from the perspective of microbial functional diversity for chemical fertilizer reduction, wheat yield increase, and soil fertility improvement in dryland soil. We conducted a long-term field experiment with seven fertilization treatments in winter wheat cultivation area of loess tableland in south Shanxi Province, including straw charcoal fertilizer (SF), bacterial fertilizer (BF), organic fertilizer (OF), humic acid fertilizer (HF), monitoring fertilizer (MF), farmer fertilizer (FF) and no fertilizer (CK). We employed Biolog-ECO microplate technique to investigate the differences of carbon source utilization capacity and functional diversity of soil microorganisms. The results showed that all the fertilization treatments could improve the metabolic activity and functional diversity of soil microbial community. Carbon source utilization was the most efficient in SF, with the overall soil microbial utilization ability of the 31 carbon sources and the utilization ability of different guilds of carbon sources being improved. Functional diversity, richness, and dominance based on microbial carbon sources utilization were significantly higher in SF treatment than that under other five treatments, and the evenness was higher than BF. Results of principal component analysis (PCA) and biclustering heatmap analysis showed that different fertilization treatments had significant effects on the metabolic function of microbial community. SF treatment could promote the functional diversity of soil microbial community, especially for the utilization of carbohydrates, carboxylic acids and amino acids. In conclusion, straw charcoal fertilizer had positive effects on soil microbial activity in wheat soil of loess tableland in south Shanxi Province.

Key words: dry tableland in south Shanxi Province, fertilization practice, Biolog-ECO microplate, microbial functional diversity, straw charcoal

LIU Lianlian, SHANG Yanmeng, ZHANG Jie, LI Tingliang, XIE Yinghe, XIE Junyu, LI Li’na, HONG Jianping. Effects of different fertilization treatments on soil microbial functional diversity of dry tableland wheat field in south Shanxi Province.[J]. Chinese Journal of Applied Ecology, 2023, 34(3): 671-678.

References

[1] 李廷亮, 谢英荷, 高志强, 等. 黄土高原旱地小麦覆膜增产与氮肥增效分析. 中国农业科学, 2018, 51(14): 2735-2746
[2] 赵广才, 常旭虹, 王德梅, 等. 中国小麦生产发展潜力研究报告. 作物杂志, 2012(3): 1-5
[3] 郭胜利, 周印东, 张文菊, 等. 长期施用化肥对粮食生产和土壤质量性状的影响. 水土保持研究, 2003(1): 16-22
[4] Hao MD, Fan J, Wang QJ, et al. Wheat grain yield and yield stability in a long-term fertilization experiment on the Loess Plateau. Pedosphere, 2007, 17: 257-264
[5] Zhang WF, Cao GX, Li XL, et al. Closing yield gaps in China by empowering smallholder farmers. Nature, 2016, 537: 671-674
[6] 王嘉豪, 李廷亮, 黄璐, 等. 秸秆还田替代化肥对黄土旱塬小麦产量及水肥利用的影响. 水土保持学报, 2022, 36(3): 236-243, 251
[7] 李春越, 常顺, 钟凡心, 等. 种植模式和施肥对黄土旱塬农田土壤团聚体及其碳分布的影响. 应用生态学报, 2021, 32(1): 191-200
[8] 姚远, 马泉, 郑国利, 等. 不同秸秆还田方式对小麦产量及土壤养分与生理活性的影响. 麦类作物学报, 2021, 41(8): 1-10
[9] 高会议, 郭胜利, 刘文兆, 等. 黄土旱塬区冬小麦不同施肥处理的土壤呼吸及土壤碳动态. 生态学报, 2009, 29(5): 2551-2559
[10] Horrigue W, Dequiedt S, Prevost-Boure NC, et al. Predictive model of soil molecular microbial biomass. Ecological Indicators, 2016, 64: 203-211
[11] Coban O, de Deyn GB, van der Ploeg M. Soil microbiota as game-changers in restoration of degraded lands. Science, 2022, 375: 990
[12] Sun AQ, Jiao XY, Chen QL, et al. Microbial communities in crop phyllosphere and root endosphere are more resistant than soil microbiota to fertilization. Soil Biology and Biochemistry, 2021, 153: 108113
[13] Singh JS, Gupta VK. Soil microbial biomass: A key soil driver in management of ecosystem functioning. Science of the Total Environment, 2018, 634: 497-500
[14] 邢亚薇, 李春越, 刘津, 等. 长期施肥对黄土旱塬农田土壤微生物丰度的影响. 应用生态学报, 2019, 30(4): 1351-1358
[15] 邢鹏飞, 武晓森, 高圣超, 等. 不同施肥处理对玉米-小麦轮作土壤微生物群落功能多样性的影响. 微生物学杂志, 2016, 36(1): 22-29
[16] 顾美英, 唐光木, 葛春辉, 等. 不同秸秆还田方式对和田风沙土土壤微生物多样性的影响. 中国生态农业学报, 2016, 24(4): 489-498
[17] Tian SY, Zhu BJ, Yin R, et al. Organic fertilization promotes crop productivity through changes in soil aggregation. Soil Biology and Biochemistry, 2022, 165: 108533
[18] 李廷亮, 孟丽霞, 谢英荷, 等. 黄土旱塬垄膜沟播小麦产量及土壤微生物量对施肥的响应. 应用与环境生物学报, 2018, 24(4): 805-812
[19] Schjonning P, Christensen BT, Carstensen B. Physical and chemical properties of a sandy loam receiving animal manure, mineral fertilizer or no fertilizer for 90 years. European Journal of Soil Science, 1994, 45: 257-268
[20] 丰娟. 腐植酸及腐植酸类肥料的应用进展. 宜春学院学报, 2009, 31(6): 103-104
[21] 章孜亮, 刘金山, 王朝辉, 等. 基于土壤氮素平衡的旱地冬小麦监控施氮. 植物营养与肥料学报, 2012, 18(6): 1387-1396
[22] 曹寒冰, 王朝辉, 师渊超, 等. 渭北旱地冬小麦监控施氮技术的优化. 中国农业科学, 2014, 47(19): 3826-3838
[23] Govaerts B, Mezzalama M, Unno Y, et al. Influence of tillage, residue management, and crop rotation on soil microbial biomass and catabolic diversity. Applied Soil Ecology, 2007, 37: 18-30
[24] Garau G, Castaldi P, Santona L, et al. Influence of red mud, zeolite and lime on heavy metal immobilization, culturable heterotrophic microbial populations and enzyme activities in a contaminated soil. Geoderma, 2007, 142: 47-57
[25] 马克平, 刘玉明. 生物群落多样性的测度方法. Ⅰ. α多样性的测度方法(下). 生物多样性, 1994, 2(4): 231-239
[26] Simpson EH. Measurement of diversity. Nature, 1949, 163: 688
[27] 崔佩佩, 武爱莲, 王劲松, 等. 不同施肥处理对高粱根际土壤微生物功能多样性的影响. 华北农学报, 2018, 33(5): 195-202
[28] 梁楚涛, 张娇阳, 艾泽民, 等. 黄土丘陵区不同施肥处理对土壤微生物特性的影响. 生态学报, 2018, 38(10): 3592-3602
[29] 周运来, 张振华, 范如芹, 等. 小麦秸秆不同还田方式下土壤微生物碳代谢多样性特征. 生态与农村环境学报, 2017, 33(10): 913-920
[30] 张功臣, 赵征宇, 王波, 等. 生物质炭和微生物菌剂配施对设施土壤理化特性及黄瓜产量和品质的影响. 江苏农业科学, 2019, 47(18): 155-159
[31] Ma LJ, Kong FX, Wang Z, et al. Growth and yield of cotton as affected by different straw returning modes with an equivalent carbon input. Field Crops Research, 2019, 243: 107616
[32] 张奇茹, 刘冰, 谢英荷, 等. 秸秆炭、硅钾肥对旱地土壤有机碳组分及小麦产量的影响. 山西农业科学, 2021, 49(3): 332-337
[33] 王晶. 棉花秸秆炭对土壤微生物群落组成和功能的影响. 硕士论文. 石河子: 石河子大学, 2020
[34] 赵占辉, 张丛志, 蔡太义, 等. 不同稳定性有机物料对砂姜黑土理化性质及玉米产量的影响. 中国生态农业学报, 2015, 23(10): 1228-1235
[35] Pokharel P, Ma, Z, Chang SX. Biochar increases soil microbial biomass with changes in extra- and intracellular enzyme activities: A global meta-analysis. Biochar, 2020, 2: 65-79
[36] Gul S, Whalen JK, Thomas BW, et al. Physico-chemical properties and microbial responses in biochar-amended soils: Mechanisms and future directions. Agriculture, Ecosystems and Environment, 2015, 206: 46-59
[37] 孙涛, 朱新萍, 李典鹏, 等. 不同原料生物炭理化性质的对比分析. 农业资源与环境学报, 2017, 34(6): 543-549
[38] 陈龙池, 廖利平, 汪思龙, 等. 根系分泌物生态学研究. 生态学杂志, 2002, 21(6): 57-62
[39] Lehmann J. A handful of carbon. Nature, 2007, 447: 143-144
[40] 张祎彤, 苏柳方, 冯晓龙, 等. 成本收益视角下的秸秆还田效益分析. 中国人口·资源与环境, 2022, 32(3): 169-176