Effects of chlorobenzene stress on seedling growth and cell division of Vicia faba

Abstract

Abstract: Effects of 1, 2,4-trichlorobenzene (TCB) stress on seedling growth, cell division and chromosomal aberration frequency of root-tip cells of Vicia faba were studied. The results indicated that the growth of the root length and mitotic index of root tip cells were successively decreased and even stopped with the increase of TCBconcentrations and treatment duration. Numerical and structural chromosomal aberrations at metaphase and anaphase of root-tip cells in Vicia faba seedlings were produced by 50～300μg·g^-1TCBtreatment for 12～96h. The percentage of c-mitosis, chromosomal bridge and chromosomal asymmetry array in root tip cells exposed to 50～100μg·g^-1 TCB for 12～24h was up to 1.0～10.3%. The percentage of chromosomal stickiness (S), chromosomal stickiness + chromosomal breakage (S+B), chromosomal stickiness + chromosomal ring (S+R), chromosomal stickiness + chromosomal asymmetry array (S+A) and chromosomal stickiness + chromosomal bridge (S+Be) in root tip cells reached 47.9～88.9%, and 18.1～29.6% for different kinds of chromosomal breakage at 300μg·g^-1 TCB for 12～96h. Thus,the chromosomal aberration of root tip cells in Vicia faba seedlings could be used as a sensitive biomarker of monitoring soil contaminated with TCB.

CLC Number:

X173

LIU Wan, ZHOU Qixing, LI Peijun, SUN Tieheng, TAI Peidong, XU Huaxia, ZHANG Chungui, ZHANG Hairong . Effects of chlorobenzene stress on seedling growth and cell division of Vicia faba[J]. Chinese Journal of Applied Ecology, 2003, (4): 585-588.

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