3种啮齿动物抗生素抗性基因多样性比较

doi:10.13287/j.1001-9332.202401.031

应用生态学报 ›› 2024, Vol. 35 ›› Issue (1): 229-236.doi: 10.13287/j.1001-9332.202401.031

3种啮齿动物抗生素抗性基因多样性比较

初鹏飞¹, 邢洁¹, 王声泽¹, 李婷¹, 乔征磊¹, 原宝东^2*

¹牡丹江师范学院生命科学与技术学院, 黑龙江牡丹江 157011;
²聊城大学生命科学学院, 山东聊城 252000

收稿日期:2023-06-19 接受日期:2023-10-24 出版日期:2024-01-18 发布日期:2024-03-21
通讯作者: * E-mail: yuanbao365@163.com
作者简介:初鹏飞, 男, 1999年生, 硕士研究生。主要从事动物生态学研究。E-mail: chupengfei1999@163.com
基金资助:
山东省自然科学基金面上项目(ZR2023MC016)

Comparison on the diversity of antibiotic resistance genes of three rodent species

CHU Pengfei¹, XING Jie¹, WANG Shengze¹, LI Ting¹, QIAO Zhenglei¹, YUAN Baodong^2*

¹College of Life Sciences and Technology, Mudanjiang Normal University, Mudanjiang 157011, Heilongjiang, China;
²College of Life Sciences, Liaocheng University, Liaocheng 252000, Shandong, China

Received:2023-06-19 Accepted:2023-10-24 Online:2024-01-18 Published:2024-03-21

摘要/Abstract

摘要： 抗生素抗性基因(ARGs)作为全球新型污染物受到广泛关注,其产生的原因主要是抗生素的滥用。为探究大林姬鼠、棕背鼠平和黑线姬鼠的抗生素抗性基因多样性,采用宏基因组测序分析来自黑龙江牡丹峰的17只大林姬鼠、17只棕背鼠平和三道关的9只黑线姬鼠的抗生素抗性基因多样性。从3种啮齿动物中共检测到19种类型及248个亚类的ARGs。棕背鼠平和黑线姬鼠差异显著的ARGs有7个,差异极显著的有5个;大林姬鼠和黑线姬鼠差异显著的ARGs有7个,差异极显著的有4个;棕背鼠平和大林姬鼠差异显著的ARGs有4个,差异极显著的有5个。3种啮齿动物丰度较高的抗生素抗性基因大类为大环内酯类、林可酰胺类、四环素类、β-内酰胺类。ARGs已广泛存在于3种啮齿动物体内,不同物种之间抗生素抗性基因的多样性存在显著差异,产生差异的原因可能是由于3种啮齿动物的分布区不同并存在食性分化。本研究可以为啮齿动物抗生素抗性基因的后续研究提供依据,并加深对ARGs传播危害的认识。

关键词: 棕背鼠平, 大林姬鼠, 黑线姬鼠, 抗生素抗性基因, 宏基因组测序

Abstract: Antibiotic resistance genes (ARGs) have attracted widespread attention as a new global pollutant, mainly due to the abuse of antibiotics. To investigate the diversity of ARGs in three rodent species, we used metagenomic sequencing analysis to analyze the diversity of antibiotic resistance genes of 17 individuals of Apodemus peninsulae and 17 individuals of Myodes rufocanus collected from Mudanfeng, and nine individuals of Apodemus agrarius collected from Sandaoguan. A total of 19 types and 248 subclasses of ARGs were detected in the three rodent species. Seven ARGs showed significant difference and five ARGs showed extremely significant difference between M. rufocanus and A. agrarius. Seven ARGs showed significant difference and four ARGs showed extremely significant difference between A. peninsulae and A. agrarius. Four ARGs showed significant difference and five ARGs showed extremely significant difference between M. rufocanus and A. peninsulae. ARGs showing high abundance in three rodents were macrolides, lincoamides, tetracyclines, and β-lactams. ARGs were widely distributed in the three rodent species. The significant differences in ARGs among different species might be due to the different distribution areas and their diet differentiation. The study could provide a basis for further studies of ARGs in mice and improve the understanding of the harm of ARGs transmission.

Key words: Myodes rufocanus, Apodemus peninsulae, Apodemus agrarius, antibiotic resistance gene, metagenomic sequencing

初鹏飞, 邢洁, 王声泽, 李婷, 乔征磊, 原宝东. 3种啮齿动物抗生素抗性基因多样性比较[J]. 应用生态学报, 2024, 35(1): 229-236.

CHU Pengfei, XING Jie, WANG Shengze, LI Ting, QIAO Zhenglei, YUAN Baodong. Comparison on the diversity of antibiotic resistance genes of three rodent species[J]. Chinese Journal of Applied Ecology, 2024, 35(1): 229-236.

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3种啮齿动物抗生素抗性基因多样性比较

Comparison on the diversity of antibiotic resistance genes of three rodent species

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