应用生态学报 ›› 2023, Vol. 35 ›› Issue (5): 1408-1418.doi: 10.13287/j.1001-9332.202405.031
张根1,2*, 陈宝锐2, 潘璐璐1,2, 王慧3,4, 杨波3,4, 卜钦鹏2
收稿日期:
2023-10-07
接受日期:
2024-03-12
出版日期:
2024-05-18
发布日期:
2024-11-18
通讯作者:
*E-mail: zhanggen1988@163.com
作者简介:
张 根, 男, 1988年生, 博士研究生。主要从事生态学与环境毒理学研究。E-mail: zhanggen1988@163.com
基金资助:
ZHANG Gen1,2*, CHEN Baorui2, PAN Lulu1,2, WANG Hui3,4, YANG Bo3,4, BU Qinpeng2
Received:
2023-10-07
Accepted:
2024-03-12
Online:
2024-05-18
Published:
2024-11-18
摘要: 在农业上,抗生素广泛用于动物的疾病预防和治疗。但抗生素的过度使用,甚至滥用,使得抗生素残留和抗生素耐药性问题日渐严重。抗生素通过食物链的生物富集和放大,将影响生态环境安全,并最终危害人体健康。传统的抗生素检测技术存在程序繁琐、操作复杂、耗时长等一系列问题,难以满足即时、高效和准确的现场检测需求。因此,为应对抗生素引起的食品安全问题、规范抗生素在农业上的使用,建立农产品抗生素的快速检测技术显得十分重要。本文综述了世界主要国家地区抗生素在养殖业的使用和管理情况,以及抗生素在农产品中的残留特征和对生物体及环境的危害,归纳了近5年内农产品中抗生素快速检测技术的发展情况,对各项快速检测技术的优缺点进行了对比,最后对未来发展方向进行了展望。本文可为农产品抗生素管控和即时检测提供借鉴。
张根, 陈宝锐, 潘璐璐, 王慧, 杨波, 卜钦鹏. 农产品抗生素残留现状及快速检测方法研究进展[J]. 应用生态学报, 2023, 35(5): 1408-1418.
ZHANG Gen, CHEN Baorui, PAN Lulu, WANG Hui, YANG Bo, BU Qinpeng. Research status and rapid detection methods of antibiotic residues in agricultural products[J]. Chinese Journal of Applied Ecology, 2023, 35(5): 1408-1418.
[1] Rhouma M, Soufi L, Cenatus S, et al. Current insights regarding the role of farm animals in the spread of antimicrobial resistance from a one health perspective. Veterinary Sciences, 2022, 9: 480 [2] Caneschi A, Bardhi A, Barbarossa A, et al. The use of antibiotics and antimicrobial resistance in veterinary medicine, a complex phenomenon: A narrative review. Antibiotics, 2023, 12: 487 [3] Tang Y, Lou X, Yang G, et al. Occurrence and human health risk assessment of antibiotics in cultured fish from 19 provinces in China. Frontiers in Cellular and Infection Mmicrobiology, 2022, 12: 964283 [4] 王国兰, 冯金露, 罗玲, 等. 污水处理厂中四环素和磺胺类抗生素抗性基因的分布、传播及去除. 应用生态学报, 2019, 30(8): 2875-2882 [5] Mann A, Nehra K, Rana JS, et al. Antibiotic resistance in agriculture: Perspectives on upcoming strategies to overcome upsurge in resistance. Current Research in Microbial Sciences, 2021, 2: 100030 [6] More SJ. European perspectives on efforts to reduce antimicrobial usage in food animal production. Irish Veterinary Journal, 2020, 11: S1-S12 [7] 蔡天贵, 张龙, 张晋东. 抗生素抗性基因的生态风险研究进展. 应用生态学报, 2022, 33(5): 1435-1440 [8] Zellweger RM, Carrique-Mas J, Limmathurotsakul D, et al. A current perspective on antimicrobial resistance in Southeast Asia. Journal of Antimicrobial Chemotherapy, 2017, 72: 2963-2972 [9] Cuong NV, Padungtod P, Thwaites G, et al. Antimicrobial usage in animal production: A review of the literature with a focus on low- and middle-income countries. Antibiotics, 2018, 7: 75 [10] Nhung NT, Cuong NV, Thwaites G, et al. Antimicrobial usage and antimicrobial resistance in animal production in Southeast Asia: A review. Antibiotics, 2016, 5: 37 [11] Nuangmek A, Rojanasthien S, Patchanee P, et al. Knowledge, attitudes and practices toward antimicrobial usage: A cross-sectional study of layer and pig farm owners/managers in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, May 2014 to February 2016. Korean Journal of Veterinary Research, 2018, 58: 17-25 [12] Pham-Duc P, Cook MA, Cong-Hong H, et al. Know-ledge, attitudes and practices of livestock and aquaculture producers regarding antimicrobial use and resistance in Vietnam. PLoS One, 2019, 14(9): e0223115 [13] Om C, Mclaws ML. Antibiotics: Practice and opinions of Cambodian commercial farmers, animal feed retailers and veterinarians. Antimicrobial Resistance & Infection Control, 2016, 5: 42 [14] Ström G, Boqvist S, Albihn A, et al. Antimicrobials in small-scale urban pig farming in a lower middle-income country: Arbitrary use and high resistance levels. Antimicrobial Resistance & Infection Control, 2018, 7: 35 [15] Nuangmek A, Rojanasthien S, Yamsakul P, et al. Perspectives on antimicrobial use in pig and layer farms in thailand: Legislation, policy, regulations and potential. Veterinary Integrative Sciences, 2020, 19: 1-21 [16] Chauhan AS, George MS, Chatterjee P, et al. The social biography of antibiotic use in smallholder dairy farms in India. Antimicrobial Resistance & Infection Control, 2018, 7: 60 [17] Suyamud B, Chen Y, Quyen DTT, et al. Antimicrobial resistance in aquaculture: Occurrence and strategies in Southeast Asia. Science of the Total Environment, 2024, 907: 167942 [18] Van Boeckel TP, Brower C, Gilbert M, et al. Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112: 5649-5654 [19] 农业农村部. 农业农村部关于印发《全国兽用抗菌药使用减量化行动方案(2021—2025 年)》的通知[EB/OL]. (2021-10-25) [2024-03-08]. http://www.moa.gov.cn/xw/bmdt/202110/t20211025_6380448.htm [20] 农业农村部. 兽医公报[EB/OL]. (2019-12-20) [2024-03-08]. http://www.moa.gov.cn/gk/sygb/202003/P020200320559902047100.pdf [21] Xu J, Sangthong R, Mcneil E, et al. Antibiotic use in chicken farms in northwestern China. Antimicrobial Resistance and Infection Control, 2020, 9: 10 [22] Fang J, Gong G, Yuan J, et al. Antibiotic use in pig farming and its associated factors in L County in Yunnan, China. Veterinary Medicine and Science, 2021, 7: 440-454 [23] National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheris. Report on the Japanese Veterinary Antimicrobial Resistance Monitoring System 2016-2017. Tokyo: Ministry of Agriculture, Forestry and Fisheris of Japan, 2020 [24] Shoji T, Sato N, Fukuda H, et al. Clinical implication of the relationship between antimicrobial resistance and infection control activities in Japanese hospitals: A principal component analysis-based cluster analysis. Anti-biotics, 2022, 11: 229 [25] Lim SK, Lee JE, Lee HS, et al. Trends in antimicrobial sales for livestock and fisheries in Korea during 2003-2012. Korean Journal of Veterinary Science, 2014, 54: 81-86 [26] Kirchhelle C. Pharming animals: A global history of antibiotics in food production (1935-2017). Palgrave Communications, 2018, 4: 96 [27] Burch D. Use of antibiotics in animals and people. Vete-rinary Record, 2015, 177: 549-550 [28] Schmerold I, Van Geijlswijk I, Gehring R. European regulations on the use of antibiotics in veterinary medicine. European Journal of Pharmaceutical Sciences, 2023, 189: 106473 [29] Purnhagen KP, Clemens S, Eriksson D, et al. Europe's farm to fork strategy and its commitment to biotechnology and organic farming: Conflicting or complementary goals. Trends in Plant Science, 2021, 26: 600-606 [30] Speksnijder DC, Mevius DJ, Bruschke CJM, et al. Reduction of veterinary antimicrobial use in the Netherlands: The Dutch Success Model. Zoonoses and Public Health, 2015, 62: 79-87 [31] Aarestrup F. Get pigs off antibiotics. Nature, 2012, 486: 465-466 [32] Wallinga D, Smit LM, Davis MF, et al. A review of the effectiveness of current US policies on antimicrobial use in meat and poultry production. Current Environmental Health Reports, 2022, 9: 339-354 [33] Administration US Food and Drug. 2009 Summary Report on Antimicrobials Sold or Distributed for Use in Food-Producing Animals. Washington DC: US FDA, 2014 [34] Administration US Food and Drug. Final Rule to Collect Antimicrobial Sales and Distribution Information by Animal Species. Washington DC: US FDA, 2020 [35] Mathers JJ, Flick SC, Cox LA. Longer-duration uses of tetracyclines and penicillins in U.S. food-producing animals: Indications and microbiologic effects. Environment International, 2011, 37: 991-1004 [36] Sneeringer S, Short G, Maclachlan M, et al. Impacts on livestock producers and veterinarians of FDA policies on use of medically important antibiotics in food animal production. Applied Economic Perspectives and Policy, 2020, 42: 674-694 [37] Singer RS, Porter LJ, Schrag NFD, et al. Estimates of on-farm antimicrobial usage in turkey production in the United States, 2013-2017. Zoonoses and Public Health, 2020, 67: 36-50 [38] Wang H, Ren L, Yu X, et al. Antibiotic residues in meat, milk and aquatic products in Shanghai and human exposure assessment. Food Control, 2017, 80: 217-225 [39] Lee HJ, Cho SH, Shin D, et al. Prevalence of antibiotic residues and antibiotic resistance in isolates of chicken meat in Korea. Korean Journal for Food Science of Animal Resources, 2018, 38: 1055-1063 [40] Jammoul A, El Darra N. Evaluation of antibiotics residues in chicken meat samples in Lebanon. Antibiotics, 2019, 8: 69 [41] Al-Ghamdi MS, Al-Mustafa ZH, El-Morsy F, et al. Residues of tetracycline compounds in poultry products in the eastern province of Saudi Arabia. Public Health, 2000, 114: 300-304 [42] Yang Y, Qiu W, Li Y, et al. Antibiotic residues in poultry food in Fujian Province of China. Food Additives & Contaminants: Part B, 2020, 13: 177-184 [43] Zhang Y, Lu J, Yan Y, et al. Antibiotic residues in cattle and sheep meat and human exposure assessment in southern Xinjiang, China. Food Science & Nutrition, 2021, 9: 6152-6161 [44] Moghadam MM, Amiri M, Riabi HRA, et al. Evaluation of antibiotic residues in pasteurized and raw milk distributed in the South of Khorasan-e Razavi Province, Iran. Journal of Clinical and Diagnostic Research, 2016, 10: FC31-FC35 [45] Orso D, Floriano L, Ribeiro LC, et al. Simultaneous determination of multiclass pesticides and antibiotics in honey samples based on ultra-high performance liquid chromatography-tandem mass spectrometry. Food Analy-tical Methods, 2016, 9: 1638-1653 [46] Geng J, Liu X, Wang J, et al. Accumulation and risk assessment of antibiotics in edible plants grown in contaminated farmlands: A review. Science of the Total Environment, 2022, 853: 158616 [47] Pan M, Wong CKC, Chu LM. Distribution of antibiotics in wastewater-irrigated soils and their accumulation in vegetable crops in the Pearl River Delta, Southern China. Journal of Agricultural and Food Chemistry, 2014, 62: 11062-11069 [48] Pan M, Chu LM. Transfer of antibiotics from wastewater or animal manure to soil and edible crops. Environmental Pollution, 2017, 231: 829-836 [49] Bacanlı M, Baçaran N. Importance of antibiotic residues in animal food. Food and Chemical Toxicology, 2019, 125: 462-466 [50] Hautekeete ML. Hepatotoxicity of antibiotics. Acta Gastroenterol Belg, 1995, 58: 290-296 [51] Darwish WS, Eldaly EA, El-Abbasy MT, et al. Anti-biotic residues in food: The African scenario. Japanese Journal of Veterinary Research, 2013, 61: S13-S22 [52] Treiber FM, Beranek-Knauer H. Antimicrobial residues in food from animal origin: A review of the literature focusing on products collected in stores and markets worldwide. Antibiotics, 2021, 10: 534 [53] Puangseree J, Jeamsripong S, Prathan R, et al. Resis-tance to widely-used disinfectants and heavy metals and cross resistance to antibiotics in Escherichia coli isolated from pigs, pork and pig carcass. Food Control, 2021, 124: 107892 [54] Arsène MMJ, Podoprigora IV, Davares AKL, et al. Antibacterial activity of grapefruit peel extracts and green-synthesized silver nanoparticles. Veterinary World, 2021, 14: 1330-1341 [55] Zhang Y, Wang X, Yin X, et al. Toxicity assessment of combined fluoroquinolone and tetracycline exposure in zebrafish (Danio rerio). Environmental Toxicology, 2016, 31: 736-750 [56] Zhang SQ, Li P, Zhao XL, et al. Hepatotoxicity in carp (Cyprinus carpio) exposed to environmental levels of norfloxacin (NOR): Some latest evidences from transcriptomics analysis, biochemical parameters and histopathological changes. Chemosphere, 2021, 283: 131210 [57] Jakobsson HE, Jernberg C, Andersson AF, et al. Short-term antibiotic treatment has differing long-term impacts on the human throat and gut microbiome. PLoS One, 2010, 5(3): e9836 [58] Raymann K, Moran NA. The role of the gut microbiome in health and disease of adult honey bee workers. Current Opinion in Insect Science, 2018, 26: 97-104 [59] Jie Z, Xia H, Zhong SL, et al. The gut microbiome in atherosclerotic cardiovascular disease. Nature Communications, 2017, 8: 845 [60] Rocha DC, Da Silva Rocha C, Tavares DS, et al. Vete-rinary antibiotics and plant physiology: An overview. Science of the Total Environment, 2021, 767: 144902 [61] Marques RZ, Wistuba N, Brito JCM, et al. Crop irrigation (soybean, bean, and corn) with enrofloxacin-contaminated water leads to yield reductions and antibiotic accumulation. Ecotoxicology and Environmental Safety, 2021, 216: 112193 [62] Pan M, Chu LM. Phytotoxicity of veterinary antibiotics to seed germination and root elongation of crops. Ecotoxicology and Environmental Safety, 2016, 126: 228-237 [63] Jia WL, Song C, He LY, et al. Antibiotics in soil and water: Occurrence, fate, and risk. Current Opinion in Environmental Science & Health, 2023, 32: 100437 [64] Ye H, Li S, Xi Y, et al. Highly sensitive determination of antibiotic residues in aquatic products by high-performance liquid chromatography-tandem mass spectrometry. Antibiotics, 2022, 11: 1427 [65] 黄孟丽, 张博文, 江改青, 等. ASE净化-HPLC-MS/MS法测定动物源性食品中12种多肽类抗生素. 食品科技, 2023, 48(5): 278-286 [66] 邱稀木, 朱晓华, 边文冀, 等. 高效液相色谱-串联质谱法测定河蟹肝胰腺组织中林可酰胺类抗生素残留. 福建农业学报, 2021, 36(10): 1231-1237 [67] 张璐, 姜伟. 基于磺酸基共价有机框架快速测定牛奶中氟喹诺酮类抗生素残留. 食品工业科技, 2023, 44(7): 269-277 [68] 王露, 司晓萍, 唐辉, 等. 禽畜肉中7种抗生素残留检测. 分析试验室, 2019, 38(7): 854-858 [69] 李涛, 谭璐, 徐文泱, 等. UPLC-Q/Orbitrap MS法检测水产品中12种抗生素残留. 食品工业, 2020, 41(5): 300-303 [70] 李雨薇, 张洪昌, 胡双庆, 等. 双水相-超高效液相色谱串联质谱法测定猪肉中17种抗生素. 东华大学学报: 自然科学版, 2023, 49(2): 159-166 [71] 吴婉琴, 范小龙, 黄坤, 等. 超高效液相色谱-串联质谱法测定鸡肉中9种大环内酯类抗生素. 食品安全质量检测学报, 2021, 12(1): 34-42 [72] 薛荣旋, 卢丽明, 黄诚, 等. QuEChERS-超高效液相色谱-串联质谱法测定水产品中6种氟喹诺酮类抗生素残留量. 中国食品卫生杂志, 2020, 32(5): 519-523 [73] 李朔, 张璨, 马玲, 等. QuEChERS结合超高效液相色谱-串联质谱法同步测定鱼肉制品中24种磺胺类抗生素. 食品工业科技, 2022, 43(9): 301-308 [74] 季宝成, 侯铸琛, 任承雨, 等. 基于硅烷化三聚氰胺海绵的改良QuEChERS结合UPLC-MS/MS快速测定猪肉中49种抗生素多残留. 食品科学, 2024, 45(2): 290-298 [75] 颜伟华, 周莹, 郭浩炜, 等. UPLC-MS/MS快速筛查豆芽中27种植物生长调节剂和抗生素类药物. 食品科学, 2021, 42(12): 302-308 [76] 龚蕾, 韩智, 曹琦, 等. 超高效液相色谱串联质谱法测定蔬菜中31种抗生素. 食品安全质量检测学报, 2021, 12(1): 43-49 [77] 陈乾, 刘洋, 肖丽君, 等. 超高效液相色谱串联质谱法同时测定叶菜中13种抗生素. 环境科学, 2020, 41(2): 952-961 [78] 张今君, 夏慧丽, 高海波, 等. 液相色谱—串联三重四极杆复合线性离子阱质谱法测定水生蔬菜中45种抗生素. 食品与机械, 2022, 38(11): 68-75 [79] Hu X, Zhao J, Cheng X, et al. Polydopamine-mediated quantity-based magnetic relaxation sensing for the rapid and sensitive detection of chloramphenicol in fish samples. Food Research International, 2022, 162: 111919 [80] 刘倩, 张航, 蒋开拓, 等. 便携式水产品四环素含量检测装置研制. 农业工程学报, 2023, 39(5): 8-14 [81] Zhang H, Pan Q, Cai W, et al. C-doped ZnO nanocomposites molecularly imprinted photoelectrochemical sensor for ultrasensitive and selective detection of oxytetracycline in milk. Food Chemistry, 2023, 426: 136535 [82] Wu S, Mao J, Zhang Y, et al. Sensitive electrochemical detection of enrofloxacin in eggs based on carboxylated multi-walled carbon nanotubes-reduced graphene oxide nanocomposites: Molecularly imprinted recognition versus direct electrocatalytic oxidation. Food Chemistry, 2023, 413: 135579 [83] Zhang L, Wang J, Deng J, et al. A novel fluorescent ‘turn-on' aptasensor based on nitrogen-doped graphene quantum dots and hexagonal cobalt oxyhydroxide nanoflakes to detect tetracycline. Analytical and Bioanalytical Chemistry, 2020, 412: 1343-1351 [84] Wang W, Deng P, Liu X, et al. A CsPbBr3 quantum dots/ultra-thin BN fluorescence sensor for stability and highly sensitive detection of tetracycline. Microchemical Journal, 2021, 162: 105876 [85] Li F, Wu Y, Chen D, et al. Sensitive dual-labeled electrochemical aptasensor for simultaneous detection of multi-antibiotics in milk. International Journal of Hydrogen Energy, 2021, 46: 23301-23309 [86] Wang Y, Zhang B, Guo M, et al. Rapid detection of cordycepin in food by surface-enhanced Raman technique. Journal of Future Foods, 2023, 3: 24-28 [87] Jin M, Wang X, Russel M, et al. Towards the rapid detection of multiple antibiotics in eggs by surface-enhanced Raman spectroscopy coupled with hollow fiber micro-extraction. Microchemical Journal, 2022, 181: 107743 [88] Jiang Y, Sun DW, Pu H, et al. Ultrasensitive analysis of kanamycin residue in milk by SERS-based aptasensor. Talanta, 2019, 197: 151-158 [89] Moreno V, Adnane A, Salghi R, et al. Nanostructured hybrid surface enhancement Raman scattering substrate for the rapid determination of sulfapyridine in milk samples. Talanta, 2019, 194: 357-362 [90] Zhang Y, Liao T, Wang G, et al. An ultrasensitive NIR-IIa' fluorescence-based multiplex immunochromatographic strip test platform for antibiotic residues detection in milk samples. Journal of Advanced Research, 2023, 50: 25-34 [91] Wu SW, Wang MY, Liu BH, et al. Sensitive enzyme-linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food. Journal of Food Safety, 2020, 40: e12759 [92] Hendrickson OD, Byzova NA, Zvereva EA, et al. Sensitive lateral flow immunoassay of an antibiotic neomycin in foodstuffs. Journal of Food Science and Techno-logy, 2021, 58: 292-301 [93] Huang L, Liu G, Fu Y. Recent developments in biosensing strategies for the detection of small molecular contaminants to ensure food safety in aquaculture and fisheries. Trends in Food Science & Technology, 2023, 133: 15-27 [94] Mahmoudpour M, Ezzati Nazhad Dolatabadi J, Torbati M, et al. Nanomaterials and new biorecognition molecules based surface plasmon resonance biosensors for mycotoxin detection. Biosensors and Bioelectronics, 2019, 143: 111603 [95] Fang B, Hu S, Wang C, et al. Lateral flow immunoassays combining enrichment and colorimetry-fluorescence quantitative detection of sulfamethazine in milk based on trifunctional magnetic nanobeads. Food Control, 2019, 98: 268-273 [96] Zheng J, He L. Surface-enhanced raman spectroscopy for the chemical analysis of food. Comprehensive Reviews in Food Science and Food Safety, 2014, 13: 317-328 [97] Kneipp K, Wang Y, Kneipp H, et al. Population pumping of excited vibrational states by spontaneous surface-enhanced Raman scattering. Physical Review Letters, 1996, 76: 2444-2447 [98] Kannan PK, Late DJ, Morgan H, et al. Recent deve-lopments in 2D layered inorganic nanomaterials for sensing. Nanoscale, 2015, 7: 13293-13312 [99] Chen Y, Wang Y, Liu L, et al. A gold immunochro-matographic assay for the rapid and simultaneous detection of fifteen β-lactams. Nanoscale, 2015, 7: 16381-16388 [100] Li Y, Li J, Huang H, et al. Rapid quantitative detection for multiple antibiotics in honey using a quantum dot microsphere immunochromatographic strip. Food Control, 2021, 130: 108256 |
[1] | 初鹏飞, 邢洁, 王声泽, 李婷, 乔征磊, 原宝东. 3种啮齿动物抗生素抗性基因多样性比较 [J]. 应用生态学报, 2024, 35(1): 229-236. |
[2] | 王文洁, 于丽明, 邵梦莹, 贾延天, 刘柳青青, 马小涵, 郑宇, 刘一帆, 张瑛臻, 罗先香, 李锋民, 郑浩. 畜禽养殖环境中抗生素抗性基因污染的研究进展 [J]. 应用生态学报, 2023, 34(5): 1415-1429. |
[3] | 李雅, 殷丽萍, 刘丹, 梁云权, 潘瑛. 中国抗生素污染现状及对浮游生物的影响 [J]. 应用生态学报, 2023, 34(3): 853-864. |
[4] | 温丽联, 宋金明, 李学刚, 马骏, 戴佳佳, 袁华茂, 段丽琴, 王启栋. 氟喹诺酮类抗生素的环境污染及其对微生物介导氮循环的影响 [J]. 应用生态学报, 2023, 34(11): 3114-3126. |
[5] | 蔡天贵, 张龙, 张晋东. 抗生素抗性基因的生态风险研究进展 [J]. 应用生态学报, 2022, 33(5): 1435-1440. |
[6] | 王玉洁, 唐宇嘉, 张亚平, 张妙月, 储双双, 仇荣亮. 生物炭对土壤中抗生素抗性基因的阻控潜力及机制研究进展 [J]. 应用生态学报, 2022, 33(11): 3116-3126. |
[7] | 陈莫莲, 安新丽, 杨凯, 朱永官. 土壤噬菌体及其介导的抗生素抗性基因水平转移研究进展 [J]. 应用生态学报, 2021, 32(6): 2267-2274. |
[8] | 张薇, 张萌, 陈凯, 金玉贺, 阎卉依, 胡艳美, 金兰淑. 恩诺沙星和铜复合污染对蚯蚓消化酶活性的影响 [J]. 应用生态学报, 2019, 30(6): 2049-2055. |
[9] | 文汉卿,史俊**,寻昊,邓慧萍. 抗生素抗性基因在水环境中的分布、传播扩散与去除研究进展 [J]. 应用生态学报, 2015, 26(2): 625-635. |
[10] | 路杨1,2,刘秀位1,2,张喜英1**. 农产品水足迹研究进展 [J]. 应用生态学报, 2015, 26(10): 3207-3214. |
[11] | 陈琳琳,李宝泉**. 新型抗生素抗性基因传播元件ISCR及其生态风险 [J]. 应用生态学报, 2015, 26(10): 3215-3225. |
[12] | 余亮亮,蔡银莺**. 基于农户受偿意愿的农田生态补偿——以湖北省京山县为例 [J]. 应用生态学报, 2015, 26(1): 215-223. |
[13] | 杨凤霞1,毛大庆2**,罗义3,汪庆4,母全华4. 环境中抗生素抗性基因的水平传播扩散 [J]. 应用生态学报, 2013, 24(10): 2993-3002. |
[14] | 李伟明,鲍艳宇**,周启星. 四环素类抗生素降解途径及其主要降解产物研究进展 [J]. 应用生态学报, 2012, 23(08): 2300-2308. |
[15] | 柴如山,牛耀芳,朱丽青,王欢,章永松. 大气CO2浓度升高对农产品品质影响的研究进展 [J]. 应用生态学报, 2011, 22(10): 2765-2775. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||