不同遗传型土生空团菌-马尾松菌根苗耐Cd能力综合评价

doi:10.13287/j.1001-9332.202410.008

应用生态学报 ›› 2024, Vol. 35 ›› Issue (10): 2677-2687.doi: 10.13287/j.1001-9332.202410.008

不同遗传型土生空团菌-马尾松菌根苗耐Cd能力综合评价

张骁^1,2, 闫添艺¹, 张玉虎¹, 武奥琳², 谢荣樟³, 张桃香¹, 杨文浩^2*

¹福建农林大学林学院森林共生生物学国际联合实验室, 福州 350002;
²福建农林大学资源与环境学院, 福州 350002;
³三明市三元区林业局, 福建三明 365000

收稿日期:2024-04-18 接受日期:2024-08-15 出版日期:2024-10-18 发布日期:2025-04-18
通讯作者: * E-mail: whyang@fafu.edu.cn
作者简介:张骁, 男, 2001年生, 硕士研究生。主要从事土壤重金属污染植物修复研究。E-mail: zx2193447224@foxmail.com
基金资助:
国家自然科学基金面上项目(42077132)和福建省林业厅科学技术基金(KLb20004A)

Comprehensive evaluation of resistance to Cd stress in mycorrhizal seedlings formed by different genotypes of Cenococcum geophilum and Pinus massoniana

ZHANG Xiao^1,2, YAN Tianyi¹, ZHANG Yuhu¹, WU Aolin², XIE Rongzhang³, ZHANG Taoxiang¹, YANG Wenhao^2*

¹International Joint Laboratory of Forest Symbiosis Biology, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
²College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
³Sanming Sanyuan District Forestry Bureau, Sanming 365000, Fujian, China

Received:2024-04-18 Accepted:2024-08-15 Online:2024-10-18 Published:2025-04-18

摘要/Abstract

摘要： 为筛选具有优异耐Cd能力的土生空团菌-马尾松菌根苗,探究土生空团菌促进马尾松抗Cd胁迫的机理,本研究将分离自不同宿主的9种土生空团菌分别侵染马尾松形成菌根苗,在Cd污染土壤中培育3个月,对生物量、根系结构和光合作用的各项指标进行主成分分析,并结合隶属函数对各菌根苗的耐Cd能力进行了综合评价。结果表明: Cd胁迫下菌根苗的干重和鲜重分别是对照的1.0～2.1倍和1.0～1.4倍;土生空团菌改善了马尾松菌根苗的根系结构,各菌根苗的根体积和根平均直径分别是对照的1.5～2.3和1.0～1.3倍;土生空团菌显著增强了菌根苗的光合作用,其中菌根苗Cg-HS3净光合速率、气孔导度、蒸腾速率和胞间CO₂浓度分别是对照的11.8、3.5、3.0和0.4倍;土生空团菌显著降低了菌根苗对Cd的转运,各菌根苗转运系数是对照的30.4%～73.0%。通过计算各菌根苗耐Cd能力综合评价D值,并进行组间连接聚类分析,将各菌根苗划分为Cd敏感型(Cg-YS4、Cg-HS2、Cg-YS1、Cg-HS1)、低耐Cd型(Cg-HS3、Cg-QQ、Cg-YS3、Cg-YS2)和高耐Cd型(Cg-QG)。总体而言,菌根苗Cg-QG和Cg-HS3可以作为抵抗Cd胁迫环境进行矿区生态恢复的优良材料。

关键词: 土生空团菌, 马尾松, 耐Cd性评价

Abstract: To select the mycorrhizal seedlings of Pinus massoniana with excellent Cd-resistance and explore the mechanism of Cenococcum geophilum promoting the resistance to Cd stress of P. massoniana, nine species of C. geophilum isolated from different hosts infected P. massoniana to form mycorrhizal seedlings, were cultured in Cd-contaminated soil for three months. We conducted the principal component analysis (PCA) on biomass, root structure, and photosynthesis, and evaluated the Cd tolerance of mycorrhizal seedlings by membership function. The results showed that dry and fresh weight of mycorrhizal seedlings under Cd stress were 1.0-2.1 and 1.0-1.4 times of that under the control. Root structure of P. massoniana mycorrhizal seedlings was improved by C. geophilum, with root volume and average root diameter being 1.5-2.3 and 1.0-1.3 times of that under the control. Photosynthesis of mycorrhizal seedlings was significantly enhanced by C. geophilum, with photosynthetic rate, stomatal conductance, transpiration rate, and intercellular CO₂ concentration of Cg-HS3 being 11.8, 3.5, 3.0, and 0.4 times of that under the control, respectively. The transport of Cd by mycorrhizal seedlings was significantly reduced by C. geophilum, which was 30.4%-73.0% of that under the control. Based on the D value of comprehensive evaluation of Cd tole-rance of mycorrhizal seedlings, and cluster analysis of inter-group linkage, the mycorrhizal seedlings were classified into Cd-sensitive type (Cg-YS4, Cg-HS2, Cg-YS1, Cg-HS1), low Cd-tolerance type (Cg-HS3, Cg-QQ, Cg-YS3, Cg-YS2), and high Cd-tolerance type (Cg-QG). In conclusion, Cg-QG and Cg-HS3 can be used as excellent materials to resist Cd stress and restore the ecological environment in mining area.

Key words: Cenococcum geophilum, Pinus massoniana, evaluation of resistance to Cd stress

张骁, 闫添艺, 张玉虎, 武奥琳, 谢荣樟, 张桃香, 杨文浩. 不同遗传型土生空团菌-马尾松菌根苗耐Cd能力综合评价[J]. 应用生态学报, 2024, 35(10): 2677-2687.

ZHANG Xiao, YAN Tianyi, ZHANG Yuhu, WU Aolin, XIE Rongzhang, ZHANG Taoxiang, YANG Wenhao. Comprehensive evaluation of resistance to Cd stress in mycorrhizal seedlings formed by different genotypes of Cenococcum geophilum and Pinus massoniana[J]. Chinese Journal of Applied Ecology, 2024, 35(10): 2677-2687.

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不同遗传型土生空团菌-马尾松菌根苗耐Cd能力综合评价

Comprehensive evaluation of resistance to Cd stress in mycorrhizal seedlings formed by different genotypes of Cenococcum geophilum and Pinus massoniana

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