Abstract: A phosphate solubilizing fungus was isolated from the rhizosphere of tomato in greenhouse in Liaozhong County, Liaoning Province, Northeast China. The strain was identified as a new strain of Penicillium oxalicum by morphological characteristics and ITS rDNA sequence comparison, and then was named PSF1. Strain PSF1 could utilize various carbon sources such as glucose, sucrose, lactose, galactose, soluble starch and nitrogen sources such as ammonium sulfate, ammonium chloride, ammonium nitrate, potassium nitrate, urea for growth and metabolism, with an efficient phosphate solubilizing capacity. It grew well and had a high ability of phosphate solubilization under the conditions of C/N 10:1-60:1 and initial pH 7-8. Strain PSF1 had strong acid production ability, with the pH of culture mediums decreasing from 7.00-7.50 to 2.06-4.87 during the culture process. The highest phosphate solubilizing capacity in four phosphorus sources mediums was tricalcium phosphate (869.62 mg·L^-1) > phosphate rock power (233.56 mg·L^-1) > aluminum phosphate (44.77 mg·L^-1) > iron phosphate (28.42 mg·L^-1). Results from Pearson correlation analysis showed that there were significant negative correlations between the changes of phosphate solubili-zing capacity and pH in tricalcium phosphate, phosphate rock power and iron phosphate mediums, but no significant correlation in aluminum phosphate medium. Strain PSF1 had strong phosphate solu-bilizing capacity and wide growing conditions, thus would have strong phosphate solubilizing capacity in soil.