铜绿假单胞菌对镉胁迫苗期水稻根系活力及叶片生理特性的影响

doi:10.13287/j.1001-9332.201908.037

应用生态学报 ›› 2019, Vol. 30 ›› Issue (8): 2767-2774.doi: 10.13287/j.1001-9332.201908.037

铜绿假单胞菌对镉胁迫苗期水稻根系活力及叶片生理特性的影响

汪敦飞¹, 郑新宇^1,2, 肖清铁^1,2, 王微¹, 林瑞余^1,2*

¹福建农林大学生命科学学院福建省农业生态过程与安全监控重点实验室, 福州 350002;
²福建农林大学作物生态与分子生理学福建省高校重点实验室, 福州 350002

收稿日期:2018-12-19 出版日期:2019-08-15 发布日期:2019-08-15
通讯作者: * E-mail: lrylin2004@163.com
作者简介:汪敦飞,男,1993年生,硕士研究生.主要从事环境污染微生物修复研究.E-mail:notwang2018@163.com
基金资助:
国家重点研发计划项目(2017YFD0800900)、国家科技基础性工作专项(2015FY111300)和福建省自然科学基金项目(2009J01056)

Effects of Pseudomonas aeruginosa on root activity and leaf physiological characteristics in rice (Oryza sativa L.) seedling under cadmium stress

WANG Dun-fei¹, ZHENG Xin-yu^1,2, XIAO Qing-tie^1,2, WANG Wei¹, LIN Rui-yu^1,2*

¹College of Life Sciences, Fujian Agriculture and Forestry University, Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fuzhou 350002, China;
²Fujian Provincal Universities Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Received:2018-12-19 Online:2019-08-15 Published:2019-08-15
Contact: * E-mail: lrylin2004@163.com

摘要/Abstract

摘要： 为揭示耐镉铜绿假单胞菌缓解镉胁迫水稻的生理效应,以无镉处理为对照,通过添加菌液、空载体、菌剂及20 μmol·L^–1 Cd进行水培试验,分析了菌株对苗期水稻根系活力及叶片生理特性的影响.结果表明: 镉胁迫显著抑制了水稻的根系活力,降低了叶片光合效率、抗氧化酶活性及可溶性蛋白、类黄酮与总酚含量,提高了叶片丙二醛(MDA)和超氧阴离子(O₂^-·)含量.与镉处理相比,添加菌液、菌剂处理的水稻根系活力分别提高了36.1%～42.5%、49.4%～53.0%;叶片净光合速率提高了118.5%～147.1%、137.6%～156.9%;可溶性蛋白含量提高了37.0%～49.3%、37.7%～72.6%.菌剂处理的水稻叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性分别比Cd处理提高了36.9%～42.6%、82.7%～92.6%、43.3%～52.2%,菌液处理的SOD、POD、 CAT则分别比Cd处理提高了25.8%～36.6%、40.9%～55.9%、24.0%～29.2%,菌剂对水稻叶片抗氧化酶的促进效应显著高于菌液;菌剂、菌液处理的水稻叶片MDA含量分别比Cd处理降低了44.8%～54.7%、29.4%～41.9%;O₂^-·含量减少了9.9%～10.2%、3.0%～7.1%;菌剂处理后类黄酮、总酚含量分别比Cd处理提高了125.4%～135.7%、100.8%～119.4%;菌液处理后则分别提高了139.4%～146.7%、115.0%～134.7%.可见,铜绿假单胞菌及其菌剂通过提高苗期水稻根系活力、光合作用促进了苗期水稻的生长.铜绿假单胞菌通过增强水稻抗氧化酶活性、提高类黄酮和总酚等抗氧化物质含量,表现出显著的缓解镉胁迫效应.

Abstract: To reveal the physiological effects of rice alleviated by cadmium-tolerant Pseudomonas aeruginosa under cadmium stress condition, the influences of bacterial strian on the root vigor and leaf physiological characteristics were analyzed under a set of hydroponic experiments involving adding bacteria suspension, empty carrier, microbial inoculum with 20 μmol·L^-1Cd. Cadmium-free treatment as control. The results showed that the root vigor was significantly inhibited, leaf photosynthetic rate decreased, and the contents of soluble protein, flavonoid and total phenols in rice leaves were reduced, while the contents of malondialdehyde (MDA) and superoxide anion(O₂^-·) increased significantly under cadmium stress condition. Compared with cadmium treatment, root vigors of rice were increased by 36.1%-42.5% and 49.4%-53.0% respectively in bacteria suspension and microbial inoculum additions, net photosynthetic rates in leaves were increased by 118.5%-147.1% and 137.6%-156.9%, and the contents of soluble protein were increased by 37.0%-49.3% and 37.7%-72.6%, respectively. For the bacteria suspension treatment, the activities of SOD, POD and CAT in leaves were increased by 25.8%-36.6%, 40.9%-55.9%, 24.0%-29.2%, and the activities of SOD, POD and CAT in leaves under microbial inoculum treatment were increased by 36.9%-42.6%, 82.7%-92.6% and 43.3%-52.2%, respectively, with the stimulative effects on antioxidation enzymes in rice leaves being higher than those of bacteria suspension. Compared with cadmium treatment, the contents of MDA and O₂^-· in rice leaves were reduced by 44.8%-54.7%, 29.4%-41.9% and 9.9%-10.2%, 3.0%-7.1% in microbial inoculum and bacteria suspension treatments, respectively. In contrast, the contents of flavonoids and total phenols were increased by 125.4%-135.7%, 100.8%-119.4% and 139.4%-146.7%, 115.0%-134.7%, respectively. In summary, P. aeruginosa and the microbial inoculum could promote rice seedling growth by improving root vigor and photosynthetic rate, as well as the contents of flavonoids and total phenols, which led to the fact that P. aeruginosa could significantly alleviate the stress of cadmium on rice.

汪敦飞, 郑新宇, 肖清铁, 王微, 林瑞余. 铜绿假单胞菌对镉胁迫苗期水稻根系活力及叶片生理特性的影响[J]. 应用生态学报, 2019, 30(8): 2767-2774.

WANG Dun-fei, ZHENG Xin-yu, XIAO Qing-tie, WANG Wei, LIN Rui-yu. Effects of Pseudomonas aeruginosa on root activity and leaf physiological characteristics in rice (Oryza sativa L.) seedling under cadmium stress[J]. Chinese Journal of Applied Ecology, 2019, 30(8): 2767-2774.

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铜绿假单胞菌对镉胁迫苗期水稻根系活力及叶片生理特性的影响

Effects of Pseudomonas aeruginosa on root activity and leaf physiological characteristics in rice (Oryza sativa L.) seedling under cadmium stress

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