Screening, identification and phosphate-solubilizing characteristics of phosphate-solubilizing bacteria strain D2 (Pantoea sp.)in rhizosphere of Pinus tabuliformis in iron tailings yard.

doi:10.13287/j.1001-9332.201611.003

Abstract

Abstract: Two strains of phosphate-solubilizing bacteria were isolated from the rhizosphere of Pinus tabuliformis in iron tailings vegetation restoration areas in Malan Town, Qianan City, Hebei Pro-vince. The bacterial strain D2 with strong phosphate-solubilizing capacity was obtained via screening with plate and shake flask. Based on the morphology, physiology and biochemistry, and the sequence analysis of 16S rDNA, the D2 was identified as a member of Pantoea sp. A fermentation experiment was conducted to investigate the effect of carbon and nitrogen sources on the phosphate-solubilizing capacity of the strain D2; under different nitrogen sources, the organic acids in liquid culture, as well as their types and contents were determined by high performance liquid chromatography. The results showed that the strain D2 was capable of efficiently solubilizing tricalcium phosphate, and the highest value of available phosphorus was up to 392.13 mg·L^-1 in liquid culture. The strain D2 displayed the strongest phosphate-solubilizing capability when glucose and ammonium sulfate were used as carbon and nitrogen sources in the culture media, respectively. Under varied nitrogen sources, the resulting organic acids and their types and contents were different. When the nitrogen source in culture media was ammonium sulfate, ammonium chloride, potassium nitrate, sodium nitrate or ammonium nitrate, all four organic acids, including oxalic acid, formic acid, acetic acid and citric acid, were produced. In addition, malic acid was uniquely produced when ammonium sulfate, ammonium chloride or ammonium nitrate was used as the nitrogen source. By Pearson’s correlation analysis, a significant positive correlation between the acetic acid content and the available phosphorus content was found (r=0.886, P<0.05), suggesting that acetic acid produced by strain D2 played an important role in promoting inorganic phosphorus dissolution, which was most likely to be one of the important phosphate-solubilizing mechanisms of the strain.

WANG Jun-juan, YAN Ai-hua, WANG Wei, LI Ji-quan, LI Yu-ling. Screening, identification and phosphate-solubilizing characteristics of phosphate-solubilizing bacteria strain D2 (Pantoea sp.)in rhizosphere of Pinus tabuliformis in iron tailings yard.[J]. Chinese Journal of Applied Ecology, 2016, 27(11): 3705-3711.

References

[1] Huang M-H (黄铭洪), Luo Y-M (骆永明). Land remediation and ecological restoration of mine land. Acta Pedologica Sinica (土壤学报), 2003, 40(2): 161-169 (in Chinese)
[2] Xiao L-G (肖力光), Yi J-H (伊晋宏), Cui Z-X (崔正旭). Iron tailings comprehensive utilization at home and abroad. Journal of Jilin Institute of Architecture and Civil Engineering (吉林建筑工程学院学报), 2010, 27(4): 22-25 (in Chinese)
[3] Johnson MS, Bradshaw AD. Ecological principles for the restoration of disturbed and degraded land. Applied Bio-logy, 1979, 4: 141-200.
[4] Wang J-J (王俊娟), Yang H-B (杨何宝), Wang W (王薇), et al. Ameliorative effects of Medicago sativa L. inoculated with rhizobium and fertilization on the growth substrate of iron tailings. Journal of Soil and Water Conservation (水土保持学报), 2016, 30(1): 272-277 (in Chinese)
[5] Li X-Y (李晓莹), Xu X-H (徐学华), Guo J (郭江), et al. Effects of different forestation species on the substrate physicochemical properties and soil fauna in iron tailings. Acta Ecologica Sinica (生态学报), 2014, 34(20): 5747-5755 (in Chinese)
[6] Zhang Y-Y (张云翼), Zou B-Y(邹碧莹). Research progress of phosphate-solubilizing bacteria in soil. Modern Agricultural Science and Technology (现代农业科技), 2008(15): 182-183 (in Chinese)
[7] Yang Y-J (杨艳菊), Wang G-L (王改兰), Zhang H-P (张海鹏), et al. Status quo of phosphate solubilizing microorganisms and its application in agriculture. Hunan Agricultural Sciences (湖南农业科学), 2012(11): 23-25 (in Chinese)
[8] Qin L-J (覃丽金), Wang Z-H (王真辉), Chen Q-B (陈秋波). Advances of researches on rhisosphere phosphate-solubilizing microorganism. Journal of South China University of Tropical Agriculture (华南热带农业大学学报), 2006, 12(2): 44-46 (in Chinese)
[9] Zhu P-M (朱培淼), Yang X-M (杨兴明), Xu Y-C (徐阳春), et al. High effective phosphate-solubilizing bacteria: Their isolation and promoting effect on corn seedling growth. Chinese Journal of Applied Ecology (应用生态学报), 2007, 18(1): 107-112 (in Chinese)
[10] Nautiyal CS. An efficient microbiological growth medium for screening phosphate-solubilizing microorganisms. FEMS Microbiology Letters, 1999, 170: 265-270
[11] Cheng L-J (程丽娟), Xue Q-H (薛泉宏). Laboratory Manual of Microbiology. Beijing: Science Press, 2012 (in Chinese)
[12] Bao S-D (鲍士旦). Soil and Agricultural Chemistry Analysis. 2^nd Ed. Beijing: China Agriculture Press, 2000 (in Chinese)
[13] Dong X-Z (东秀珠), Cai M-Y (蔡妙英). Manual of Systematic Determinative Bacteriology. Beijing: Science Press, 2001 (in Chinese)
[14] Du S (杜森), Gao X-Z (高祥照). Technical Specifications in Soil Analysis. Beijing: China Agriculture Press, 2006 (in Chinese)
[15] Qiao Z-W (乔志伟), Hong J-P (洪坚平), Xie Y-H (谢英荷), et al. Screening, identification and phosphate-solubilizing characteristics of Rahnella sp. phosphate-solubilizing bacteria in calcareous soil. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(8): 2294-2300 (in Chinese)
[16] Liu H (刘辉), Wu X-Q (吴小芹), Ren J-H (任嘉红). Phosphate-dissolving characteristics and growth promoting effect of Pseudomonads fluorescent JW-JSI on poplar seedlings. Scientia Silvae Sinicae (林业科学), 2013, 49(9): 112-117 (in Chinese)
[17] Shi F-C (史发超), Yin Z-W (殷中伟), Jiang H-M (江红梅), et al. Screening, identification of P-dissolving fungus P83 strain and its effects on phosphate solubilization and plant growth promotion. Acta Microbiologica Sinica (微生物学报), 2014, 54(11): 1333-1343 (in Chinese)
[18] Chen Q (陈倩), Liu S-J (刘善江), Bai Y (白杨), et al. Screening and identification of phosphate-solubilizing bacteria from reclaimed soil in Shanxi mining area. Journal of Plant Nutrition and Fertilizer (植物营养与肥料学报), 2014, 20(6): 1505-1516 (in Chinese)
[19] Zhang L-Z (张丽珍), Feng L-L (冯利利), Meng Q-X (蒙秋霞), et al. Isolation, identification, real-time PCR investigation of an endophytic phosphate-solubili-zing bacteria from Caragana korshinskii Kom. roots. Acta Ecologica Sinica (生态学报), 2013, 33(13): 3941-3946 (in Chinese)
[20] Chung H, Park M, Madhaiyan M, et al. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biology and Biochemistry, 2005, 37: 1970-1974
[21] Reyes I, Bernier L, Simard RR, et al. Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microbiology Ecology, 1999, 28: 281-290
[22] Wan L (万璐), Kang L-H (康丽华), Liao B-W (廖宝文), et al. Mangrove PSB: isolation, culture and phosphate-dissolving ability. Forest Research (林业科学研究), 2014, 17(1): 89-94 (in Chinese)
[23] Wenzel CL, Ashford AE, Summerell BA, et al. Phosphate solubilizing bacteria associated with proteoid roots of seedlings of waratah. New Phytologist, 1994, 128:487-496
[24] Wang Y-K (王岳坤), Yu F (于飞), Tang C-R (唐朝荣). Screening and molecular identification of phosphate-solubilizing bacteria in rhizosphere soils in Hainan ecosystem. Acta Microbiologica Sinica (微生物学报), 2009, 49(1): 64-71 (in Chinese)
[25] Paul NB, Rao WVBS. Phosphate-dissolving bacteria in the rhizosphere of some cultivated legumes. Plant and Soil, 1971, 35: 127-132
[26] Son HJ, Park GT, Cha MS, et al. Solubilization of insoluble inorganic phosphates by a novel salt- and pH-to-lerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresource Technology, 2006, 97: 204-210
[27] Yu Q-Y (于群英), Ma Z-Y (马忠友), Wang J-F (汪建飞), et al. Screening of phosphorus solubilizing bacteria and its effects on transformation of soil inorganic phosphorus fractions. Journal of Soil and Water Conservation (水土保持学报), 2012, 26(5): 103-107 (in Chinese)
[28] Liu W-G (刘文干), He Y-Q (何园球), Zhang K (张坤), et al. Isolation, identification and characterization of a strain of phosphate-solubilizing bacteria from red soil. Acta Microbiologica Sinica (微生物学报), 2012, 52(3): 326-333 (in Chinese)
[29] Goldstein AH. Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by Gram-negative bacteria// Torriani-Gorini A, Yagil E, Silver S, eds. Phosphate in Microorganisms: Cellular and Molecular Niology. Washington, DC: ASM Press, 1996: 197-203
[30] Yu W-B (虞伟斌), Yang X-M (杨兴明), Shen Q-R (沈其荣), et al. Mechanism on phosphate solubilization of Pseudomonas sp. K₃ and its phosphate solubilization ability under buffering condition. Journal of Plant Nutrition and Fertilizer (植物营养与肥料学报), 2010, 16(2): 354-361 (in Chinese)
[31] He M-X (贺梦醒), Gao Y (高毅), Hu Z-X (胡正雪), et al. Screening, identification, and phosphate-solubilizing capability of phosphate-solubilizing bacterial strain B25. Chinese Journal of Applied Ecology (应用生态学报), 2012, 23(1): 235-239 (in Chinese)