Abstract: The expansion of built-up area will cause stress effect on the regional natural ecological security pattern during urbanization process. Taking rapid expanding regions of four inland and coastal cities as study areas, including Tongzhou in Beijing, Zhengding in Hebei, Tanggu in Tianjin and Xiamen in Fujian, we constructed regional landscape stress indexes according to the principle of landscape ecology and comparatively analyzed the landscape pattern characteristics of rapid expanding regions and the differences of stress effect of artificial landscapes on four natural landscapes ecological security pattern in the process of rapid urbanization. Results showed that landscape erosion indexes of Tongzhou, Zhengding, Tanggu and Xiamen in 2015 were 1.039, 0.996, 1.239 and 0.945, respectively, which indicated that the natural landscapes were eroded significantly. Natural landscape types of those four regions presented different threatened levels. Among all natural landscape types, unused land and waters were worst threatened in Tongzhou, Zhengding and Tanggu, while in Xiamen cultivated land and waters showed the highest threat levels. The waters threat indexes of those four areas were all more than 0.743. Landscape isolation indexes of waters and unused land of the inland cities were greater than those of coastal cities, which meant water distribution of inland cities in the space was less gathered than that of coastal cities. Besides, compared with the other natural landscape, unused land and waters suffered the largest stress from artificial landscapes.