Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress.

doi:10.13287/j.1001-9332.201802.021

Abstract

Abstract: To explore the role of exogenous spermidine (Spd) in enhancing the resistance of maize to drought stress, 15% polyethylene glycol (PEG-6000) was used to simulate drought stress and with ‘Xianyu 335’ (drought-insensitive) and ‘Fenghe 1’ (drought sensitive) as the experiment materials, the effects of Spd (0.1 mmol·L^-1) on the growth, photosynthetic characteristics, chlorophyll content, osmotic adjustment substance, membrane lipid peroxidation and root activity of maize seedlings were investigated. The results showed that the application of Spd significantly promoted the growth of maize seedlings under drought stress, increased chlorophyll content, net photosynthetic rate (P_n), stomatal conductance (g_s), transpiration rate (T_r) and water use efficiency (WUE), and decreased the enhancement of intercellular carbon dioxide concentration (C_i) in ‘Fenghe 1’. Moreover, the stomatal and non-stomatal limitations of photosynthetic ability caused by drought stress in ‘Fenghe 1’ were effectively reduced by exogenous Spd. The application of Spd increased the content of proline and soluble sugar, decreased theO₂^-· generation rate, contents of H₂O₂ and MDA and cell membrane permeability, enhanced the root activity. The changes of drought-sensitive ‘Fenghe 1’ were greater than drought-tolerant ‘Xianyu 335’. These results indicated that exogenous Spd had positive effects on the seedlings to capture and converse solar energy, thus promoting photosynthesis and the growth of maize seedlings. It would also enhance the adaptability of seedlings to drought stress by reducing the production of reactive oxygen species (ROS), increasing the accumulation of osmotic adjustment substances to stabilize the cell membrane system and improving the root vigor. The positive effects of Spd was more obvious for drought-sensitive variety ‘Fenghe 1’.

LI Li-jie, GU Wan-rong, MENG Yao, WANG Yue-li, MU Jun-yi, LI Jing, WEI Shi. Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress.[J]. Chinese Journal of Applied Ecology, 2018, 29(2): 554-564.

References

[1] Shao R-X (邵瑞鑫), Li L-L (李蕾蕾), Zheng H-F (郑会芳), et al. Effects of exogenous nitric oxide on photosynthesis of maize seedlings under drought stress. Scientia Agricultura Sinica (中国农业科学), 2016, 49(2): 251-259 (in Chinese)
[2] Cai F (蔡福), Mi N (米娜), Ji R-P (纪瑞鹏), et al. Effects of drought stress and subsequent rewatering on major physiological parameters of spring maize during the key growth periods. Chinese Journal of Applied Ecology (应用生态学报), 2017, 28 (11): 3643-3652 (in Chinese)
[3] Yang X-C (杨晓晨), Ming B (明博), Tao H-B (陶洪斌), et al. Spatial distribution characteristics and impact on spring maize yield of drought in Northeast China. Chinese Journal of Eco-Agriculture (中国生态农业学报), 2015, 23(6): 758-767 (in Chinese)
[4] Zhang R-H (张仁和), Zheng Y-J (郑友军), Ma G-S (马国胜), et al. Effects of drought stress on photosynthetic traits and protective enzyme activity in maize seeding. Acta Ecologica Sinica (生态学报), 2011, 31(5): 1303-1311 (in Chinese)
[5] Du W-L (杜伟莉), Gao J (高杰), Hu F-L (胡富亮), et al. Responses of drought stress on photosynthetic trait and osmotic adjustment in two maize cultivars. Acta Agronomica Sinica (作物学报), 2013, 39(3): 530-536 (in Chinese)
[6] Shao HB, Liang ZS, Shao MA. Osmotic regulation of 10 wheat (Triticum aestivum L.) genotypes at soil water deficits. Colloids and Surfaces B: Biointerfaces, 2006, 47: 132-139
[7] Miller G, Suzuki N, Ciftci-Yilmaz S, et al. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell and Environment, 2010, 33: 453-467
[8] Roychoudhury A, Basu S, Sengupta DN. Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology, 2011, 168: 317-328
[9] Shu S, Yuan LY, Guo SR, et al. Effects of exogenous spermine on chlorophyll fluorescence, antioxidant system and ultrastructure of chloroplasts in Cucumis sativus L. under salt stress. Plant Physiology and Biochemistry, 2013, 63: 209-216
[10] Shu S, Guo SR, Sun J, et al. Effects of salt stress on the structure and function of the photosynthetic apparatus in Cucumis sativus and its protection by exogenous putrescine. Physiologia Plantarum, 2012, 146: 285-296
[11] Zhang C-M (张春梅), Zou Z-R (邹志荣), Huang Z (黄志), et al. Effects of exogenous spermidine on photosynthesis of tomato seedlings under drought stress. Agricultural Research in the Arid Areas (干旱地区农业研究), 2010, 28(3): 182-187 (in Chinese)
[12] Zhang C-M (张春梅), Zou Z-R (邹志荣), Zhang Z-X (张志新), et al. Effects of exogenous spermidine on reactive oxygen levels and antioxidative system of tomato seedling under polyethlene glycol stress. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2009, 15(3): 301-307 (in Chinese)
[13] Li Z (李州), Peng Y (彭燕), Pan M-H (潘明洪), et al. Effects of spermidine on the accumulation of osmoregulatory matter in leaves of white clover under PEG stress. Chinese Journal of Grassland (中国草地学报), 2014, 36(1): 31-36 (in Chinese)
[14] Zou Q (邹琦). Plant Physiology Experiment Guidance. Beijing: China Agriculture Press, 2003: 62-64, 72-75 (in Chinese)
[15] Li H-S (李合生). Plant Physiological and Biochemical Principles and Techniques. Beijing: Higher Education Press, 2006 (in Chinese)
[16] Nahar K, Hasanuzzaman M, Alam MM, et al. Glutathione-induced drought stress tolerance in mung bean: Coordinated roles of the antioxidant defence and methylg-lyoxal detoxification systems. AoB Plants, 2015, 7, plv069, doi: 10.1093/aobpla/plv069
[17] Zhu X-Q (朱新强), Zhang X-Y (张新颖), Shi S-L (师尚礼), et al. Comparison on the root drought resistance of four alfalfa cultivars under drought stress. Journal of Gansu Agricultural University (甘肃农业大学学报), 2012, 47(1): 103-107 (in Chinese)
[18] Wang S (王飒), Zhou Q (周琦), Zhu Z-L (祝遵凌). Physiological and biochemical characteristics of Carpinus betulus seedlings under drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2013, 33(12): 2459-2466 (in Chinese)
[19] Gitelson AA, Gritz Y, Merzlyak MN. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. Journal of Plant Physiology, 2003, 160: 271-282
[20] Yamasato A, Nagata N, Tanaka R, et al. The N-terminal domain of chlorophyllide a oxygenase confers protein instability in response to chlorophyll b accumulation in Arabidopsis. Plant Cell, 2005, 17: 1585-1597
[21] Zhou H, Guo S, An Y, et al. Exogenous spermidine delays chlorophyll metabolism in cucumber leaves (Cucumis sativus L.) under high temperature stress. Acta Phy-siologiae Plantarum, 2016, 38: 224. doi: 10.1007/s11738-016-2243-2
[22] Li X, Gong B, Xu K. Interaction of nitric oxide and polyamines involves antioxidants and physiological strategies against chilling-induced oxidative damage in Zingiber officinale Roscoe. Scientia Horticulturae, 2014, 170: 237-248
[23] Ji Y (季杨), Zhang X-Q (张新全), Peng Y (彭燕), et al. Effects of drought stress on the root growth and photosynthetic characters of Dactylis glomerata seedlings. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(10): 2763-2769 (in Chinese)
[24] Farquhar GD, Sharkey TD. Stomatal conductance and photosynthesis. Annual Review of Plant Biology, 1982, 33: 317-345
[25] Ashraf M, Harris PJC. Photosynthesis under stressful environments: An overview. Photosynthetica, 2013, 51: 163-190
[26] Shu S, Yuan LY, Guo SR, et al. Effects of exogenous spermidine on photosynthesis xanthophyll cycle and endogenous polyamines in cucumber seedlings exposed to salinity. African Journal of Biotechnology, 2012, 11: 6064-6074
[27] Madhulika S, Jitendra K, Samiksha S, et al. Roles of osmoprotectants in improving salinity and drought tolerance in plants: A review. Reviews in Environmental Science and Bio/Technology, 2015, 14: 407-426
[28] Campos KF, Carvalho K, Souza FS, et al. Drought tolerance and antioxidant enzymatic activity in transgenic ‘Swingle’ citrumelo plants over-accumulating proline. Environmental and Experimental Botany, 2011, 72: 242-250
[29] Shi HT, Ye TT, Chan ZL. Comparative proteomic and physiological analyses reveal the protective effect of exogenous polyamines in the bermudagrass (Cynodon dactylon) response to salt and drought stresses. Journal of Proteome Research, 2013, 12: 4807-4829
[30] Shi HT, Chan ZL. Improvement of plant abiotic stress tolerance through modulation of the polyamine pathway. Journal of Integrative Plant Biology, 2014, 56: 114-121
[31] Nayyar H, Gupta D. Differential sensitivity of C3 and C4 plants to water deficit stress: Association with oxidative stress and antioxidants. Environmental and Experimental Botany, 2006, 58: 106-113
[32] Hoekstra FA, Golovina EA, Buitink J. Mechanisms of plant desiccation tolerance. Trends in Plant Science, 2001, 6: 431-438
[33] Monakhova OF, Chernyad’ev II. Protective role of kartolin-4 in wheat plants exposed to soil drought. Applied Biochemistry and Microbiology, 2002, 38: 373-380
[34] Ge T-D (葛体达), Sui F-G (隋方功), Zhang J-Z (张金政), et al. Response of leaf and root membrane permeability and leaf water to soil drought in maize. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2005, 25(3): 507-512 (in Chinese)
[35] Shi J, Fu XZ, Peng T, et al. Spermine pretreatment confers dehydration tolerance of citrus in vitro plants via modulation of antioxidative capacity and stomatal response. Tree Physiology, 2010, 30: 914-922
[36] Wang K-Y (王考艳), Liu M-Y (刘美艳), Shen J (申杰), et al. Effect of exogenous spermidine on membrane lipid peroxidation and protective enzyme activities of maize root under water logging stress. Jiangsu Agricultural Sciences (江苏农业科学), 2010, 38(5): 139-141 (in Chinese)