Abstract: Land use change is an important factor affecting soil carbon and nitrogen cycle, and this is a hot spot in the study of global climate change. Based on the fixed depth method (FD) and the equivalent mass method (ESM), we explored the effects of land use change on soil organic carbon (SOC) and total nitrogen (TN) storage from the perspectives of forest reclamation and farmland vegetation restoration in Karst area. The storage of SOC and TN significantly decreased after the fore-sts were converted to grassland, eucalyptus and farmland. On average, the SOC and TN losses calculated by the FD method were 47.4% and 41.6%, respectively, the losses of SOC and TN calculated by ESM method were 54.8% and 49.7%. The SOC and TN storage increased significantly after abandonment of farmland to grassland or planting eucalyptus. On average, the SOC and TN stocks calculated based on the FD method increased by 60.5% and 49.7%, while the SOC and TN calculated by the ESM method increased by 85.5% and 70.8% respectively. The difference between FD method and ESM method was analyzed, it was concluded that FD method ignored the difference of soil bulk density after land use change. Soil bulk density was significantly increased after forest destruction. The loss of SOC and TN storage was overestimated by the FD method. Soil bulk density decreased after vegetation restoration, FD method will underestimate the enhancement of SOC and TN storage. It is suggested that the ESM method should be used to estimate the impacts of land use change on SOC and TN storage.