Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China

doi:10.13287/j.1001-9332.201910.030

Abstract

Abstract: To explore the distribution of fungal community in soil of brown coniferous forest in Daxing’anling and its relationship with soil characteristics, soil fungal community structure and diversity in three forest types, light brown coniferous forest, grass brown coniferous forest, and typical brown coniferous forest, were analyzed by high-throughput sequencing technology. The relationship between soil fungal community and soil characteristics was analyzed by R and SPSS 17.0 software. The results showed that soil physical and chemical characteristics differed significantly across the three brown coniferous forests. A total of 111 species belonged to six phyla, 23 class, 46 orders, 74 families and 88 genera were recorded in this study. Ascomycetes and basidiomycetes were dominant phyla. There were significant differences in relative abundance of phyla among three forest types. There was no significant difference in the Ace and Chao1 diversity indices among three brown coniferous forests, whereas there were significant differences in the Shannon and Simpson indices. Results from canonical correlation analysis and correlation analysis showed that both α and β diversity were significantly correlated with soil moisture, soil pH, soil organic matter, soil total nitrogen, and soil total potassium. The results indicated that soil characteristics were important factors affecting soil fungal diversity of brown coniferous forest in Xing’an larch forest in Daxing’anling.

YANG Li-bin, SUI Xin, WEI Dan, CUI Fu-xing, ZHU Dao-guang, NI Hong-wei. Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China[J]. Chinese Journal of Applied Ecology, 2019, 30(10): 3411-3418.

References

[1] Karabi M, Hamdi AB, Zenkhri S. Microbial diversity and organic matter fractions under two arid soils in Algerian Sahara. Technologies and Materials for Renewable Energy, Environment and Sustainability Beirut, Lebanon, 2016, doi: 10.1063/1.4959402
[2] Lagomarsino A, Moscatelli MC, Tizio AD, et al. Soil biochemical indicators as a tool to assess the short-term impact of agricultural management on changes in organic C in a Mediterranean environment. Ecological Indicators, 2009, 9: 518-527
[3] Handelsman J, Rondon MR, Brady SF, et al. Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products. Chemistry & Biology, 1998, 5: 245-249
[4] Yang LB, Sui X, Zhu DG, et al. Soil bacterial community structure and diversity of brown coniferous forest in Daxing’anling Mountain, China. Bangladesh Journal of Botany, 2018, 47: 677-688
[5] Levett D, Middleton P, Cole M, et al. Carbon cycling of alpine tundra ecosystems on Changbai Mountain and its comparison with arctic tundra. Science in China Earth Sciences, 2002, 45: 903-910
[6] Gao Y-G (高永刚), Zhao H-Y (赵慧颖), Gao F (高峰), et al. Climate change trend in future and its influence on wetlands in the Greater Khingan Mountains. Journal of Glaciology & Geocryology (冰川冻土), 2016, 38(1): 47-56 (in Chinese)
[7] Li C-Y (李春艳). Reseatch of the systematic ciassficaton of brown coniferous forest soil in Heilongjiang Pro-vince. Natural Science Journal of Harbin Normal University (哈尔滨师范大学自然科学学报), 1997(3): 103-109 (in Chinese)
[8] Lu Y (陆媛), Chen Y-Y (陈云明), Cao Y (曹杨), et al. C, N and P stoichiometric characteristics of plants and soil in quercus liaotungensis forest on Ziwu-ling Mountain of Loess Plateau. Journal of Soil and Water Conservation (水土保持学报), 2015, 29(3): 196-201 (in Chinese)
[9] Lyu W-Q (吕文强), Zhou C-Y (周传艳), Yan J-H (闫俊华), et al. Soil organic carbon density and its influencing factors for the forest region of Guizhou Province, China. Earth & Environment (地球与环境), 2016, 44(2): 147-153 (in Chinese)
[10] Xiong D (熊丹), Wu L-C (吴立潮), He J-N (何介南). Three major stand types of Mangshan’s soil organiccarbon distribution. Journal of Central South Forestry University (中南林业科技大学学报), 2017, 37(12): 120-126 (in Chinese)
[11] Cheng H (程欢), Gong Y-B (宫渊波), Wu Q (吴强), et al. Content and ecological stoichiometry characteristics of organic carbon, nitrogen and phosphorus of typical soils in sub-alpine/alpine mountain of western Sichuan. Journal of Natural Resources (自然资源学报), 2018, 33(1): 161-172 (in Chinese)
[12] Liu X-J (刘西军), Xu X-N (徐小牛), Chen X-L (陈学玲), et al. Study on the content of four metal elements in different forest soil. Journal of Soil & Water Conservation (水土保持学报), 2009, 23(1): 127-131 (in Chinese)
[13] Wang X-S (王雪松), Hou C-W (侯长旺), Xu T- J(许铁军), et al. Soil distribution rules and characteristics in Huzhong nature reserve. Forestry Science & Technology Information (林业科技情报), 2011, 43(4): 12-15(in Chinese)
[14] Corstanje R, Reddy KR, Prenger JP, et al. Soil microbial eco-physiological response to nutrient enrichment in a sub-tropical wetland. Ecological Indicators, 2007, 7: 277-289
[15] Wright AL, Reddy KR, Corstanje R. Patterns of heterotrophic microbial activity in eutrophic and oligotrophic peatlands. European Journal of Soil Biology, 2009, 45: 131-137
[16] Zhang W-R (张万儒). Forest Soil in China. Beijing: Science Press, 1986: 54-73 (in Chinese)
[17] Zhuang K-X (庄凯勋). Study on Natural Resources and Biodiversity of Huzhong National Nature Reserve in Heilongjiang Province. Harbin: Northeast Forestry University Press, 2010 (in Chinese)
[18] Chen Y (陈煜), Xu J-S (许金石), Zhang L-X (张丽霞), et al. Environmental interpretation of variations on forest community and understory herbaceous in Taibai Mountain, Shanxi, China. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2016, 36(4): 784-795 (in Chinese)
[19] Buee M, Reich M, Murat C, et al. 454 pyrosequencing analyses of forest soil reveal an unexpectedly high fungal diversity. New Phytologist, 2009, 184: 449-456
[20] Wang Y-Y (王艳云), Guo D-F (郭笃发). The application of 454 high-throughput sequencing technology into analysing the diversity of soil fungi in the field planting Tamarix chinensis and Angiospermae. Biotechnology Bulletin (生物技术通报), 2016, 32(7): 48-53 (in Chinese)
[21] Gao W-W (高微微), Kang Y (康颖), Lu H (卢宏), et al. Basic soil physicochemical properties and soil fungal diversity under different forest types of urban forest. Journal of Northeast Forestry University (东北林业大学学报), 2016, 44(3): 89-94,100 (in Chinese)
[22] Li C (李超), Liang J-F (梁俊峰), Zhou G-Y (周光益), et al. Diversity of soil fungi in Yangdongshan Shierdushui Nature Reserve. Mycosystema (菌物学报), 2014, 33(1): 152-161 (in Chinese)
[23] Freilich S, Kreimer A, Meilijson I, et al. The large-scale organization of the bacterial network of ecological co-occurrence interactions. Nucleic Acids Research, 2010, 38: 3857-3868
[24] Sui X (隋心), Zhang R-T (张荣涛), Zhong H-X (钟海秀), et al. Study on bacterial diversity of Deyeuxia angustifolia wetland by application of high-throughput sequencing technology in Sanjiang Plain. Soils (土壤), 2015, 47(5): 919-925 (in Chinese)
[25] Berg G, Smalla K. Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiology Ecology, 2009, 68: 1-13
[26] Shen C, Xiong J, Zhang H, et al. Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain. Soil Biology & Biochemistry, 2013, 57: 204-211
[27] Bai X-X (白晓旭), Shi R-J (史荣久), You Y-M (尤业明), et al. Bacterial community structure and diversity in soils of different forest ages and types in Baotianman forest, Henan Province, China. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(8): 2273-2281 (in Chinese)
[28] Edgar RC, Haas BJ, Clemente JC, et al. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics, 2011, 27: 2194-2200
[29] Maidak BL, Cole JR, Lilburn TG, et al. The RDP-II (ribosomal database project). Nucleic Acids Research, 2000, 28: 173-174
[30] Haegeman B, Hamelin, Jérome, Moriarty J, et al. Robust estimation of microbial diversity in theory and in practice. The ISME Journal, 2013, 7: 1092-1101
[31] Han S-Z (韩世忠), Gao R (高人), Li A-P (李爱萍), et al. Soil microbial community structure of two types of forests in the mid-subtropics of China. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(7): 2151-2158 (in Chinese)
[32] He Y-H (何苑皞), Zhou G-Y (周国英), Wang S-J (王圣洁), et al. Fungal diversity in Cunninghamia lanceolata plantation soil. Acta Ecologica Sinica (生态学报), 2014, 34(10): 2725-2736 (in Chinese)
[33] Carney KM, Matson PA. Plant Communities, soil microorganisms, and soil carbon cycling: Does altering the world belowground matter to ecosystem functioning. Ecosystems, 2005, 8: 928-940
[34] Donnison LM, Griffith GS, Bardgett RD. Determinants of fungal growth and activity in botanically diverse haymeadows: Effects of litter type and fertilizer additions. Soil Biology and Biochemistry, 2000, 32: 289-294
[35] Truu M, Juhanson J, Truu J. Microbial biomass, activity and community composition in constructed wetlands. Science of the Total Environment, 2009, 407: 3958-3971
[36] Smolander A, Kurka A, Kitunen V, et al. Microbial biomass C and N, and respiratory activity in soil of repeatedly limed and N- and P-fertilized Norway spruce stands. Soil Biology and Biochemistry, 1994, 26: 957-962
[37] Hu X-J (胡晓婧), Liu J-J (刘俊杰), Wei D (魏丹), et al.Comparison on fungal molecular ecological networks of agricultural soils with different latitudes in the black soil region of Northeast China. Chinese Journal of Applied Ecology (应用生态学报), 2018, 29(11): 303-311 (in Chinese)
[38] Zheng H (郑华), Ouyang Z-Y (欧阳志云), Wang X-K (王效科), et al. Effects of forest restoration patterns on soil microbial communities. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(11): 2019-2024 (in Chinese)
[39] Han Y-Z (韩有志), Wang Z-Q (王政权). Spatial hete-rogeneity and forest regeneration. Chinese Journal of Applied Ecology (应用生态学报), 2002, 13(5): 615-619 (in Chinese)