Abstract: With three grape varieties, Red Barbara, Summer Black and Hutai No. 8 as test mate-rials, we investigated the effects of foliar spraying of organic selenium fertilizer on greenhouse grape quality and selenium content. The results showed that spraying 12 mg·L^-1 amino acid chelated selenium-enriched foliar fertilizer on grape leaves significantly increased selenium content and the quantity and quality of grape yield, including the contents of soluble sugar, organic acid, soluble protein, soluble solids, vitamin C and proanthocyanidins. However, there was no increase in resveratrol. Among the three varieties, selenium content of Summer Black in 2017 and 2018 was increased by 36.7% and 37.1%, respectively, being higher than that of Red Barbara and Hutai No. 8. Red Barbara sprayed with selenium fertilizer had better quality due to high sugar and low acid contents, as well as high health-care components. Moreover, the selenium content of Hutai No. 8 in 2018 was 53.26 μg·kg^-1, higher than the others, indicating a stronger ability of selenium enrichment. We concluded that the increase range of Se content was larger in Summer Black, Red Barbara showed the better nutrition value and quality, and Hutai No. 8 was a suitable variety for selenium-rich grape production.