Toxic effects of polystyrene and dibutyl phthalate on purple lettuce

doi:10.13287/j.1001-9332.202109.038

Abstract

Abstract: As a carrier of environmental pollutants, microplastics have received wide concerns in recent years. However, the direct and indirect effects of the coexistence of polystyrene particles (PS) and pollutants on vegetables are still unclear. Here, the combined effects of 0.25, 0.50, 1.0 mg·mL^-1 PS and 5 mg·L^-1 dibutyl phthalate (DBP) on the biomass and biochemical indices of purple lettuce were investigated in hydroponic experiments. The results showed that the presence of PS increased the inhibition of DBP on lettuce biomass and increased O₂^-· content in roots and leaves relative to the control group with DBP alone, with positive consequences on the activities of supero-xide dismutase, ascorbic acid peroxidase, dehydroascorbate reductase and monodehydroascorbate reductase. According to transmission electron microscope analysis, plasmolysis occurred in root cells under the treatment of DBP alone, cell wall was damaged in PS-only treatment, and the negative effect was enhanced when DBP and PS coexisted. Therefore, the combined pollution of PS and DBP aggravated the toxic effect on purple lettuce.

Key words: microplastics, plasticizer, antioxidant enzymes, compound pollution, oxidative damage

WANG Sheng-li, SONG Zheng-guo, WANG Cheng-wei, LIU Yu, GAO Min-ling. Toxic effects of polystyrene and dibutyl phthalate on purple lettuce[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3335-3340.

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