Responses of soil microbial biomass nitrogen to organic fertilizer with different degrees of maturity and regu-lation to soil mineral nitrogen

doi:10.13287/j.1001-9332.202301.018

Abstract

Abstract: By combining the composting process with soil culture experiment, we conducted an experiment with four treatments, including conventional chemical fertilizer (CK), chemical fertilizer + compost maturity reaching 50% germination index (GI, the same below) organic fertilizer (CO1), chemical fertilizer + compost maturity reaching 80% GI organic fertilizer (CO2), chemical fertilizer + compost maturity reaching 100% GI organic ferti-lizer (CO3). We measured soil microbial biomass nitrogen (MBN), mineral nitrogen (NH₄⁺-N, NO₃^--N), net nitrification rate, microbial biomass carbon (MBC), dissolved organic carbon (DOC), soil urease and soil protease, aiming to reveal the regulatory effect of soil MBN on mineral nitrogen. The results showed that organic fertilizer application significantly increased MBN and NH₄⁺-N concentrations by 50.1%-62.4% and 109.9%-147.1%, reduced NO₃^--N concentration and net nitrification rate by 23.3%-46.8%, and 26.2%-51.5%, and enhanced MBC, DOC, urease and protease activities by 33.8%-69.6%, 7.4%-20.8%, 11.2%-69.0% and 9.4%-25.1%, respectively. The change ranges of CO2 and CO3 were significantly higher than CO1. Redundancy analysis (RDA) and structural equation model (SEM) results showed that the application of organic fertilizer with higher degree of maturity (GI≥80%) positively regulated soil MBC, MBN, NH₄⁺-N, and the activities of urease and protease, but had a negative effect on soil net nitrification rate. The combined application of chemical fertilizers and high decomposed organic fertilizers could significantly increase soil MBN and NH₄⁺-N contents, as well as soil urease and protease activities, but reduce soil net nitrification rate. To efficiently utilize organic solid wastes, it is recommended to use chemical fertilizer in combination of organic fertilizers with 80% decomposing degree in practical production to reduce the cost in both economy and time.

Key words: organic fertilizer with different maturity degree, microbial biomass nitrogen, mineral nitrogen, regulation.

DENG Ya-qin, XU Zhi, ZHANG Yong, WANG Yu-yun. Responses of soil microbial biomass nitrogen to organic fertilizer with different degrees of maturity and regu-lation to soil mineral nitrogen[J]. Chinese Journal of Applied Ecology, 2023, 34(1): 137-144.

References

[1] 吴荣, 刘善江, 孙昊, 等. 长期定位不同施肥方式对土壤肥力和微生物的影响. 中国土壤与肥料, 2020(4): 12-18
[2] 韩晓日, 郑国砥, 刘晓燕, 等. 有机肥与化肥配合施用土壤微生物量氮动态、来源和供氮特征. 中国农业科学, 2007, 40(4): 765-772
[3] 李世清, 李生秀. 有机物料在维持土壤微生物体氮库中的作用. 生态学报, 2001, 21(1): 136-142
[4] Li J, Marry C, Lin ZA, et al. Soil microbial community structure and function are significantly affected by long-term organic and mineral fertilization regimes in the North China Plain. Applied Soil Ecology, 2015, 96: 75-87
[5] 丁维婷, 房静静, 武雪萍, 等. 有机肥替代化肥不同比例对黑土土壤微生物学性质及春麦产量品质的影响. 中国土壤与肥料, 2021(2): 44-52
[6] Sinha MK, Sinha DP, Sinha H. Organic matter transformations in soils. Plant and Soil, 1977, 46: 579-590
[7] Liu MQ, Hu F, Chen XY, et al. Organic amendments with reduced chemical fertilizer promote soil microbial development and nutrient availability in a subtropical paddy filed: The influence of quantity, type and application time of organic amendments. Applied Soil Ecology, 2009, 42: 166-175
[8] 张勇, 徐智, 王宇蕴, 等. 有机无机配施体系中有机肥腐熟程度对化肥氮利用率的影响机制. 中国生态农业学报, 2021, 29(6): 1051-1060
[9] 李俊杰, 邹洪琴, 许发辉, 等. 土壤微生物量氮对小麦各生育期氮素形态的调控. 植物营养与肥料学报, 2021, 27(8): 1321-1329
[10] 骆坤, 胡荣桂, 张文菊, 等. 黑土有机碳、氮及其活性对长期施肥的响应. 环境科学, 2013, 34(2): 676-684
[11] 周建斌, 陈竹君, 李生秀. 土壤微生物量氮含量、矿化特性及其供氮作用. 生态学报, 2001, 21(10): 1718-1725
[12] 仇少君, 彭佩钦, 刘强, 等. 土壤微生物生物量氮及其在氮素循环中作用. 生态学杂志, 2006, 25(4): 443-448
[13] 巩闪闪, 刘晓静, 张志勇, 等. 不同施氮措施对冬小麦农田土壤酶活性和氮转化的影响. 生态环境学报, 2020, 29(11): 2215-2222
[14] 王淑平, 周广胜, 孙长占, 等. 土壤微生物量氮的动态及其生物有效性研究. 植物营养与肥料学报, 2003, 9(1): 87-90
[15] 吕凤莲, 侯苗苗, 张弘弢, 等. 塿土冬小麦-夏玉米轮作体系有机肥替代化肥比例研究. 植物营养与肥料学报, 2018, 24(1): 22-32
[16] 李燕青, 温延臣, 林治安, 等. 不同有机肥与化肥配施对氮素利用率和土壤肥力的影响. 植物营养与肥料学报, 2019, 25(10): 1669-1678
[17] 哈丽哈什·依巴提, 张丽, 陆强, 等. 猪粪堆肥与化肥不同配施方式对水稻产量和养分累积的影响. 南京农业大学学报, 2013, 36(5): 77-82
[18] Tang Z, Yu GH, Liu DY, et al. Different analysis techniques for fluorescence excitation-emission matrix spectroscopy to assess compost maturity. Chemosphere, 2011, 82: 1202-1208
[19] 王静, 王磊, 刘耀斌, 等. 长期施氮肥对黄棕壤微生物生物性状的影响及其调控因素. 中国生态农业学报, 2021, 29(5): 833-843
[20] Haynes RJ. Labile organic matter as an indicator of organic matter quality in arable and pastoral soils in New Zealand. Soil Biology and Biochemistry, 2000, 32: 211-219
[21] 李平, 郎漫. 开垦年限对黑土氮初级转化速率和净转化速率的影响. 土壤学报, 2020, 57(1): 165-173
[22] 袁颖红, 张文锋, 周际海, 等. 改良剂对旱地红壤活性有机碳及土壤酶活性的影响. 土壤, 2017, 49(5): 909-918
[23] 关松荫. 土壤酶及其研究法. 北京: 中国农业出版社, 1986
[24] 吴金水, 林启美, 黄巧云, 等. 土壤微生物生物量测定方法及其应用. 北京: 气象出版社, 2011
[25] 刘益仁, 徐阳春, 李想, 等. 有机肥部分替代化肥对土壤微生物生物量及矿质态氮含量的影响. 江西农业学报, 2009, 21(11): 70-73
[26] 蓝贤瑾, 刘益仁, 侯红乾, 等. 长期有机无机肥配施对红壤性水稻土微生物生物量和有机质结构的影响. 农业资源与环境学报, 2021, 38(5): 810-819
[27] 孙凤霞, 张伟华, 徐明岗, 等. 长期施肥对红壤微生物生物量碳氮和微生物碳源利用的影响. 应用生态学报, 2010, 21(11): 2792-2798
[28] Wang J, Zhu B, Zhang JB, et al. Mechanisms of soil N dynamics following long-term application of organic fertilizers to subtropical rain-fed purple soil in China. Soil Biology and Biochemistry, 2015, 91: 222-231
[29] 朱菜红, 董彩霞, 沈其荣, 等. 配施有机肥提高化肥氮利用效率的微生物作用机制研究. 植物营养与肥料学报, 2010, 16(2): 282-288
[30] 徐欣, 郑利远, 周珂, 等. 长期施肥对不同有机质水平黑土蛋白酶活性及氮素的影响. 中国土壤与肥料, 2019(1): 44-48
[31] 唐海明, 李超, 肖小平, 等. 有机肥氮投入比例对双季稻田根际土壤微生物生物量碳、氮和微生物熵的影响. 应用生态学报, 2019, 30(4): 1335-1343
[32] 付智丹, 周丽, 陈平, 等. 施氮量对玉米/大豆套作系统土壤微生物数量及土壤酶活性的影响. 中国生态农业学报, 2017, 25(1): 1463-1474
[33] 宋震震, 李絮花, 李娟, 等. 有机肥和化肥长期施用对土壤活性有机氮组分及酶活性的影响. 植物营养与肥料学报, 2014, 20(3): 525-533
[34] 王兴龙, 朱敏, 杨帆, 等. 配施有机肥减氮对川中丘区土壤微生物量与酶活性的影响. 水土保持学报, 2017, 31(3): 271-276
[35] 韩晓日, 邹德乙, 郭鹏程, 等. 长期施肥条件下土壤生物量氮的动态及其调控氮素营养的作用. 植物营养与肥料学报, 1996, 2(1): 16-22
[36] 张亚丽, 张娟, 沈其荣, 等. 秸秆生物有机肥的施用对土壤供氮能力的影响. 应用生态学报, 2002, 13(12): 1575-1578
[37] 金发会, 李世清, 卢红玲, 等. 石灰性土壤微生物量碳、氮与土壤颗粒组成和氮矿化势的关系. 应用生态学报, 2007, 18(12): 2739-2746
[38] 梁斌, 周建斌, 杨学云. 长期施肥对土壤微生物生物量碳、氮及矿质态氮含量动态变化的影响. 植物营养与肥料学报, 2010, 16(2): 321-326
[39] Mishra S, Di HJ, Cameron KC, et al. Gross nitrogen mineralisation rates in pastural soils and their relationships with organic nitrogen fractions microbial biomass and protease activity under glasshouse conditions. Biology and Fertility of Soils, 2005, 42: 45-53