Diversity and community assembly mechanism of soil ectomycorrhizal  fungi in urban parks of Baotou City, China

doi:10.13287/j.1001-9332.202305.007

Abstract

Abstract: Ectomycorrhizal (EM) fungi play an important role in forest ecosystems. However, little is known about the mechanisms driving diversity and community composition of soil EM fungi in urban forest parks which are intensively affected by anthropogenic activities. In this study, we investigated the EM fungal community using Illumina high-throughput sequencing with soil samples collected from three typical forest parks, including Olympic Park, Laodong Park, and Aerding Botanical Garden of Baotou City. The results showed that soil EM fungi richness index followed a pattern of Laodong Park (146.43±25.17) > Aerding Botanical Garden (102.71±15.31) > Olympic Park (68.86±6.83). Russula, Geopora, Inocybe, Tomentella, Hebeloma, Sebacina, Amanita, Rhizopogon, Amphinema, and Lactarius were the dominant genera in the three parks. EM fungal community composition was significantly different among the three parks. Results of linear discriminant analysis effect size (LEfSe) indicated that all parks had biomarker EM fungi that exhibiting significantly different abundance. The normalized stochasticity ratio (NST) and the inferring community assembly mechanisms by phylogenetic-bin-based null model analysis (iCAMP) showed that both stochastic and deterministic processes determined soil EM fungal communities in the three urban parks, with a dominant role of the stochastic process. Drift and dispersal limitation in the stochastic process and homogeneous selection in the deterministic process were the dominant ecological processes of soil EM fungal community assembly in the three urban parks.

Key words: Baotou City, urban forest park, ectomycorrhizal fungi, ecological process

WANG Yonglong, ZHANG Xuan, XU Ying, ZHAO Yanling, WANG Jiaqi, ZHANG Yujia, YANG Yanci. Diversity and community assembly mechanism of soil ectomycorrhizal fungi in urban parks of Baotou City, China[J]. Chinese Journal of Applied Ecology, 2023, 34(5): 1225-1234.

References

[1] Smith SE, Read DJ. Mycorrhizal Symbiosis. Cambridge, UK: Academic Press, 2008
[2] 郭伟, 郝汉, 张伟浩, 等. 外生菌根真菌通过调节离子平衡提高蒙古栎耐盐性. 应用生态学报, 2022, 33(12): 3303-3311
[3] 王艺, 丁贵杰. 马尾松菌根化苗木对干旱的生理响应及抗旱性评价. 应用生态学报, 2013, 24(3): 639-645
[4] 朱教君, 康宏樟, 许美玲, 等. 外生菌根真菌对科尔沁沙地樟子松人工林衰退的影响. 应用生态学报, 2007, 18(12): 2693-2698
[5] Gorzelak MA, Asay AK, Pickles BJ, et al. Inter-plant communication through mycorrhizal networks mediates complex adaptive behaviour in plant communities. AoB Plants, 2015, 7: plv050
[6] Cahanovitc R, Livne-Luzon S, Angel R, et al. Ectomycorrhizal fungi mediate belowground carbon transfer between pines and oaks. The ISME Journal, 2022, 16: 1420-1429
[7] Tedersoo L, Mett M, Ishida TA, et al. Phylogenetic relationships among host plants explain differences in fungal species richness and community composition in ectomycorrhizal symbiosis. New Phytologist, 2013, 199: 822-831
[8] Wang YL, Gao C, Chen L, et al. Host plant phylogeny and geographic distance strongly structure Betulaceae-associated ectomycorrhizal fungal communities in Chinese secondary forest ecosystems. FEMS Microbiology Eco-logy, 2019, 95: fiz037
[9] Miyamoto Y, Maximov TC, Bryanin SV, et al. Host phylogeny is the primary determinant of ectomycorrhizal fungal community composition in the permafrost ecosystem of eastern Siberia at a regional scale. Fungal Eco-logy, 2022, 55: 101117
[10] Miyamoto Y, Sakai A, Hattori M, et al. Strong effect of climate on ectomycorrhizal fungal composition: Evidence from range overlap between two mountains. The ISME Journal, 2015, 9: 1870-1879
[11] Glassman SI, Wang IJ, Bruns TD. Environmental filtering by pH and soil nutrients drives community assembly in fungi at fine spatial scales. Molecular Ecology, 2017, 26: 6960-6973
[12] Glassman SI, Peay KG, Talbot JM, et al. A continental view of pine-associated ectomycorrhizal fungal spore banks: A quiescent functional guild with a strong biogeographic pattern. New Phytologist, 2015, 205: 1619-1631
[13] Gao Q, Yang ZL. Diversity and distribution patterns of root-associated fungi on herbaceous plants in alpine meadows of southwestern China. Mycologia, 2016, 108: 281-291
[14] Gong S, Feng B, Jian SP, et al. Elevation matters more than season in shaping the heterogeneity of soil and root associated ectomycorrhizal fungal community. Microbio-logy Spectrum, 2022, 10: e01950-21
[15] 闫冰, 陆晴, 夏嵩, 等. 城市土壤微生物多样性研究进展. 生物多样性, 2022, 30(8): 191-204
[16] Mahmood S, Finlay RD, Fransson AM, et al. Effects of hardened wood ash on microbial activity, plant growth and nutrient uptake by ectomycorrhizal spruce seedlings. FEMS Microbiology Ecology, 2003, 43: 121-131
[17] Parke EL, Linderman RG, Black CH. The role of ectomycorrhizas in drought tolerance of Douglar-fir seedlings. New Phytologist, 1983, 95: 83-95
[18] Zheng W, Morris EK, Rillig MC. Ectomycorrhizal fungi in association with Pinus sylvestris seedlings promote soil aggregation and soil water repellency. Soil Biology and Biochemistry, 2014, 78: 326-331
[19] Huang JP, Li Y, Fu CB, et al. Dryland climate change: Recent progress and challenges. Reviews of Geophysics, 2017, 55: 719-778
[20] Huang JP, Yu HP, Guan XD, et al. Accelerated dryland expansion under climate change. Nature Climate Change, 2016, 6: 166-171
[21] 张旋, 杜薇, 徐颖, 等. 包头市半干旱型森林公园土壤细菌多样性与功能. 生物多样性, 2022, 30(7): 193-204
[22] Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 2010, 7: 335-336
[23] Bengtsson-Palme J, Ryberg M, Hartmann M, et al. Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Methods in Ecology and Evolution, 2013, 4: 914-919
[24] Tedersoo L, Bahram M, Põlme S, et al. Global diversity and geography of soil fungi. Science, 2014, 346: 1256688
[25] Tedersoo L, Smith ME. Lineages of ectomycorrhizal fungi revisited: Foraging strategies and novel lineages revealed by sequences from belowground. Fungal Biology Reviews, 2013, 27: 83-99
[26] Lai JS, Zou Y, Zhang JL, et al. Generalizing hierarchical and variation partitioning in multiple regression and canonical analyses using the rdacca.hp R package. Me-thods in Ecology and Evolution, 2022, 13: 782-788
[27] Toju H, Tanabe AS, Ishii HS. Ericaceous plant-fungus network in a harsh alpine-subalpine environment. Mole-cular Ecology, 2016, 25: 3242-3257
[28] Ning DL, Deng Y, Tiedje JM, et al. A general framework for quantitatively assessing ecological stochasticity. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116: 16892-16898
[29] Ning DL, Yuan MT, Wu LW, et al. A quantitative framework reveals ecological drivers of grassland microbial community assembly in response to warming. Nature Communications, 2020, 11: 4717
[30] Sloan WT, Lunn M, Woodcock S, et al. Quantifying the roles of immigration and chance in shaping prokaryote community structure. Environmental Microbiology, 2006, 8: 732-740
[31] 陈历睿, 林佳妮, 沈蓉, 等. 三峡库区马尾松林土壤真菌群落特征及影响因素. 应用生态学报, 2022, 33(9): 2397-2404
[32] Wu BW, Gao C, Chen L, et al. Host phylogeny is a major determinant of Fagaceae-associated ectomycorrhizal fungal community assembly at a regional scale. Frontiers in Microbiology, 2018, 9: 2409
[33] 李敏, 吕桂芬, 牛艳芳, 等. 内蒙古不同气候带白桦外生菌根真菌群落结构及影响因素. 生态学报, 2022, 42(12): 4847-4860
[34] Wang YL, Zhao YL, Xu Y, et al. Ectomycorrhizal fungal communities associated with Larix gemelinii Rupr. in the Great Khingan Mountains, China. PeerJ, 2021, 9: e11230
[35] Wang YL, Zhang X, Xu Y, et al. Fungal diversity and community assembly of ectomycorrhizal fungi associated with five pine species in Inner Mongolia, China. Frontiers in Microbiology, 2021, 12: 646821
[36] Yelle DJ, Ralph J, Lu F, et al. Evidence for cleavage of lignin by a brown rot basidiomycete. Environmental Microbiology, 2008, 10: 1844-1849
[37] Tedersoo L, May TW, Smith ME. Ectomycorrhizal life-style in fungi: Global diversity, distribution, and evolution of phylogenetic lineages. Mycorrhiza, 2010, 20: 217-263
[38] 王家源, 殷小琳, 任悦, 等. 毛乌素沙地樟子松外生菌根真菌多样性特征. 微生物学通报, 2020, 47(11): 3856-3867
[39] Jiao S, Chu H, Zhang B, et al. Linking soil fungi to bacterial community assembly in arid ecosystems. iMeta, 2022, 1: e2
[40] Beck S, Powell JR, Drigo B, et al. The role of stochasticity differs in the assembly of soil- and root-associated fungal communities. Soil Biology and Biochemistry, 2015, 80: 18-25

Diversity and community assembly mechanism of soil ectomycorrhizal fungi in urban parks of Baotou City, China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GUO Wei, HAO Han, ZHANG Wei-hao, HU Zeng-hui, LENG Ping-sheng. Ectomycorrhizal fungi enhance salt tolerance of Quercus mongolica by regulating ion balance [J]. Chinese Journal of Applied Ecology, 2022, 33(12): 3303-3311.
[2]	FAN Hua-ye, LI Ying, ZHANG Ting-long, GAO Huan-lin, HU Shuai. Research advances in model simulation and data assimilation of water and carbon fluxes in land surface vegetation [J]. Chinese Journal of Applied Ecology, 2020, 31(6): 2098-2108.
[3]	FENG Huan, MENG Pan-pan, DOU Qing, ZHANG Shou-xia, WANG Hai-hua, WANG Chun-yan. Advances in mechanisms of nutrient exchange between mycorrhizal fungi and host plants [J]. Chinese Journal of Applied Ecology, 2019, 30(10): 3596-3604.
[4]	WU Qi-qian, WANG Chuan-kuan. Effects of changes in seasonal snow-cover on litter decomposition and soil nitrogen dynamics in forests. [J]. Chinese Journal of Applied Ecology, 2018, 29(7): 2422-2432.
[5]	DONG Hong-juan, WU Xin-wei, WANG Hong-jiao, XIA Shan-shan, PAN Ying. Effects of spatial structure on predator-prey interactions: A review. [J]. Chinese Journal of Applied Ecology, 2017, 28(2): 712-720.
[6]	ZHAO Nan-xing, HAN Qi-sheng, HUANG Jian. Soil propagule bank of ectomycorrhizal fungi in natural forest of Pinus bungeana [J]. Chinese Journal of Applied Ecology, 2017, 28(12): 3855-3861.
[7]	TENG Ming-jun1, ZENG Li-xiong1, XIAO Wen-fa1, ZHOU Zhi-xiang2, HUANG Zhi-lin1, WANG Peng-cheng2, DIAN Yuan-yong2. Research progress on remote sensing of ecological and environmental changes in the Three Gorges Reservoir area, China. [J]. Chinese Journal of Applied Ecology, 2014, 25(12): 3683-3693.
[8]	WU Ting-juan. Effects of global change on soil fauna diversity: A review. [J]. Chinese Journal of Applied Ecology, 2013, 24(2): 581-588.
[9]	. Ecological effect of road network in the typical area of Yunnan Province based on integration of landscape pattern and process. [J]. Chinese Journal of Applied Ecology, 2009, 20(08): 1925-1931.
[10]	JIANG Yong. Micronutrient cycling and its affecting factors in forest ecosystems. [J]. Chinese Journal of Applied Ecology, 2009, 20(01): 197-204 .
[11]	LI Xiao;HUANG Yi;WEI Zhi-cheng . Degradation effect of two pure cultured ectomycorrhizal fungi on diesel. [J]. Chinese Journal of Applied Ecology, 2008, 19(07): 1579-1584 .
[12]	HUANG Guilin^1,2; HE Ping^2，3; HOU Meng¹. Present status and prospects of estuarine wetland research in China [J]. Chinese Journal of Applied Ecology, 2006, 17(09): 1751-1756 .
[13]	LIANG Zhenchun; HUANG Yi; AO Xiaolan. Pb-tolerance of ectomycorrhizal fungi from polluted and unpolluted sites under different P levels [J]. Chinese Journal of Applied Ecology, 2006, 17(06): 1081-1085 .
[14]	LIN Bo, LIU Qing, WU Yan, HE Hai, QIAO Yongkang . Dynamics of litters in artificial restoration process of subalpine coniferous forest [J]. Chinese Journal of Applied Ecology, 2004, (9): 1491-1496.
[15]	LIN Bo, LIU Qing, WU Yan, HE Hai, QIAO Yongkang . Dynamics of litters in artificial restoration process of subalpine coniferous forest [J]. Chinese Journal of Applied Ecology, 2004, (9): 1491-1496.