Change of soil labile organic carbon fractions and enzyme activities during peatland restoration in Changbai Mountains, Northeast China

doi:10.13287/j.1001-9332.202508.015

Abstract

Abstract: Peatlands are one of the most important terrestrial carbon storage reservoirs. The response of soil labile organic carbon fractions to environmental changes is a pivotal indicator for assessing the stability of soil organic carbon pools. Soil enzymes act as primary participants in the biogeochemical processes of peatlands, significantly influence the material cycling and energy flow. Taking natural peatlands, degraded peatlands, and peatlands restored for 3, 5, and 8 years in the Changbai Mountains as test objects, we examined the changes of soil labile organic carbon fractions, enzyme activities, soil physicochemical properties, and aboveground biomass during peatland restoration. The results showed that the contents of easily oxidizable organic carbon (EOC), microbial biomass carbon (MBC), and dissolved organic carbon (DOC) increased following peatland restoration. Both MBC and DOC exhi-bited a progressive increase with restoration duration, showing cumulative rises of 139.7% and 160.2%, respec-tively, after 8 years of restoration. In contrast, EOC recovered to the level comparable to natural peatland within just 3 years of restoration, exhibiting a notably rapid recovery. Restoration significantly increase the activities of β-1,4-N-acetylglucosamine glycosidase (NAG) and acid phosphatase (ACP) in the 0-10 cm soil layer. After 8 years of restoration, the activities of NAG and ACP increased by 30.1% and 84.1%, respectively. However, the activity of β-1,4-glucosidase (βG) increased by 60.8% after 3 years of restoration, decreased slightly after 5 years of restoration, and showed no significant difference between the peatland restored for 8 years and the degraded peatlands. Correlation analysis and structural equation modeling showed that soil organic carbon directly influenced soil labile organic carbon fractions, while soil labile organic carbon fractions and aboveground biomass collectively influenced soil enzyme activity. In conclusion, natural-based restoration could effectively increase soil labile organic carbon fractions and soil microbial enzyme activities, thereby promoting peatland recovery. This study would provide basic data and a reference framework for the ecological restoration and management of degraded peatlands.

Key words: peatland, soil labile organic carbon fraction, soil enzyme activity, Changbai Mountain, natural-based restoration

LI Linghui, LIU Yijun, WANG Yimeng, WANG Ming. Change of soil labile organic carbon fractions and enzyme activities during peatland restoration in Changbai Mountains, Northeast China[J]. Chinese Journal of Applied Ecology, 2025, 36(8): 2361-2369.

References

[1] Charman D. Peatlands and Environmental Change. New York, USA: John Wiley & Sons, 2002, 19: 505-507
[2] Yu ZC, Loisel J, Brosseau DP, et al. Global peatland dynamics since the Last Glacial Maximum. Geophysical Research Letters, 2010, 37: L13402
[3] Wang SR, Zhuang QL, Lahteenoja O, et al. Potential shift from a carbon sink to a source in Amazonian peatlands under a changing climate. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115: 12407-12412
[4] Wang HY, Wu JQ, Li G, et al. Changes in soil carbon fractions and enzyme activities under different vegetation types of the northern Loess Plateau. Ecology and Evolution, 2020, 10: 12211-12223
[5] Chen L, Zhou SL, Zhang Q, et al. Effect of organic material addition on active soil organic carbon and microbial diversity: A meta-analysis. Soil and Tillage Research, 2024, 241: 106128
[6] He LY, Lu SX, Wang CG, et al. Changes in soil orga-nic carbon fractions and enzyme activities in response to tillage practices in the Loess Plateau of China. Soil and Tillage Research, 2021, 209: 104940
[7] Zhou B, Zhao LX, Wang YB, et al. Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plastic-shed vegetable soils in China. Ecotoxicology and Environmental Safety, 2020, 195: 110495
[8] Monytes G, Solano-Campos F, Vega-Baudrit JR, et al. Environmentally relevant concentrations of silver nano-particles diminish soil microbial biomass but do not alter enzyme activities or microbial diversity. Journal of Hazardous Materials, 2020, 391: 122224
[9] Zhao ZH, Zhang CZ, Li F, et al. Effect of compost and inorganic fertilizer on organic carbon and activities of carbon cycle enzymes in aggregates of an intensively cultivated Vertisol. PLoS One, 2020, 15(3): e0229644
[10] Deng SR, Li JY, Du ZZ, et al. Rice ACID PHOSPHATASE 1 regulates Pi stress adaptation by maintaining intracellular Pi homeostasis. Plant, Cell and Environment, 2022, 45: 191-205
[11] Romanowicz KJ, Kane ES, Potvin LR, et al. Understanding drivers of peatland extracellular enzyme activity in the PEATcosm experiment: Mixed evidence for enzymic latch hypothesis. Plant and Soil, 2015, 397: 371-386
[12] Sinsabaugh RL, Hill BH, Follstad Shah JJ. Ecoenzyma-tic stoichiometry of microbial organic nutrient acquisition in soil and sediment. Nature, 2009, 462: 795-798
[13] Liu LF, Tian JQ, Wang HJ, et al. Stable oxic-anoxic transitional interface is beneficial to retard soil carbon loss in drained peatland. Soil Biology and Biochemistry, 2023, 181: 109024
[14] Cui J, Zhu ZK, Xu XL, et al. Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming. Soil Biology and Biochemistry, 2020, 142: 107720
[15] Georgiou K, Jackson RB, Vinduková O, et al. Global stocks and capacity of mineral-associated soil organic carbon. Nature Communications, 2022, 13: 3797
[16] Wang M, Talbot J, Moore TR. Drainage and fertilization effects on nutrient availability in an ombrotrophic peatland. Science of the Total Environment, 2018, 621: 1255-1263
[17] Bragazza L, Buttler A, Habermacher J, et al. High nitrogen deposition alters the decomposition of bog plant litter and reduces carbon accumulation. Global Change Biology, 2012, 18: 1163-1172
[18] Ma L, Zhu GF, Chen BL, et al. A globally robust relationship between water table decline, subsidence rate, and carbon release from peatlands. Communications Earth and Environment, 2022, 3: 254
[19] 鲍士旦. 土壤农化分析. 第3版. 北京: 中国农业出版社, 2000: 42-108
[20] Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 2002, 34: 1309-1315
[21] 吴金水, 林启美, 黄巧云, 等. 土壤微生物生物量测定方法及其应用. 北京: 气象出版社, 2006: 117-141
[22] Vance ED, Brookes PC, Jenkinson DS. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 1987, 19: 703-707
[23] Blair G, Lefroy R, Lisle L. Soil carbon fractions based on their degree of oxidation, and the development of a carbon management index for agricultural systems. Australian Journal of Agricultural Research, 1995, 46: 393-406
[24] Bell CW, Fricks BE, Rocca JD, et al. High-throughput fluorometric measurement of potential soil extracellular enzyme activities. Journal of Visualized Experiments, 2013, 15: e50961
[25] 马雪燕, 穆兴民, 王双银, 等. 黄土丘陵区不同植被恢复对土壤入渗影响及适宜模型研究. 水土保持学报, 2023, 37(2): 67-75
[26] Jiang XJ, Liu W, Chen C, et al. Effects of three morphometric features of roots on soil water flow behavior in three sites in China. Geoderma, 2018, 320: 161-171
[27] 邓思宇, 陈袁波, 余珂, 等. 不同类型泥炭沼泽湿地无机离子、溶解有机质的变化特征及生态学意义. 应用生态学报, 2021, 32(2): 571-580
[28] 徐飞. 垦殖与恢复对三江平原沼泽湿地土壤微生物群落结构与功能多样性的影响. 博士论文. 哈尔滨: 东北林业大学, 2017
[29] 李晨阳, 孔祥强, 董合忠. 植物吸收转运硝态氮及其信号调控研究进展. 核农学报, 2020, 34(5): 982-993
[30] Lin X, Tfaily MM, Steinweg JM, et al. Microbial community stratification linked to utilization of carbohydrates and phosphorus limitation in a boreal peatland at Marcell Experimental Forest, Minnesota, USA. Applied and Environmental Microbiology, 2014, 80: 3518-3530
[31] Oorschot MV, Gaalen NV, Maltby E, et al. Experimental manipulation of water levels in two French riverine grassland soils. Acta Oecologica, 2000, 21: 49-62
[32] Boye K, Noël V, Tfaily MM, et al. Thermodynamically controlled preservation of organic carbon in floodplains. Nature Geoscience, 2017, 10: 415-419
[33] 黄靖宇, 宋长春, 宋艳宇, 等. 湿地垦殖对土壤微生物量及土壤溶解有机碳、氮的影响. 环境科学, 2008, 29(5): 1380-1387
[34] Wang K, Sheng MY, Wang LJ, et al. Response of soil phytolith-occluded organic carbon accumulation to long-term vegetation restoration in Southwest China karst. Land Degradation and Development, 2022, 33: 3088-3102
[35] Wang J, Zhang A, Zheng YJ, et al. Long-term litter removal rather than litter addition enhances ecosystem carbon sequestration in a temperate steppe. Functional Ecology, 2021, 35: 2799-2807
[36] Cusack DF, Silver WL, Torn MS, et al. Effects of nitrogen additions on above-and belowground carbon dyna-mics in two tropical forests. Biogeochemistry, 2011, 104: 203-225
[37] Loris D, Denis L, Laurent A, et al. Hydro-ecological controls on dissolved carbon dynamics in groundwater and export to streams in a temperate pine forest. Biogeosciences, 2018, 15: 669-691
[38] 全紫曼, 漆燕, 周泽弘, 等. 山黧豆还田与氮肥减施对稻田土壤活性有机碳组分及酶活性的影响. 草业科学, 2024, 41(5): 1057-1067
[39] Bonn A, Allott T, Evans M, eds. Peatland Restoration and Ecosystem Services: Science, Policy and Practice. Cambridge, UK: Cambridge University Press, 2016: 129-150
[40] Zhang YM, Huang XY, Wang RC, et al. The distribution of long-chain n-alkan-2-ones in peat can be used to infer past changes in pH. Chemical Geology, 2020, 544: 119622
[41] Trivedi P, Delgado-Baquerizo M, Trivedi C, et al. Microbial regulation of the soil carbon cycle: Evidence from gene-enzyme relationships. The ISME Journal, 2016, 10: 2593-2604
[42] 刘文兰, 孙学刚, 焦健, 等. 黄河兰州段湿地土壤与甘蒙柽柳叶片碳氮磷生态化学计量特征. 北京林业大学学报, 2023, 45(6): 43-51
[43] 梁超, 朱雪峰. 土壤微生物碳泵储碳机制概论. 中国科学: 地球科学, 2021, 51(5): 680-695
[44] Sinsabaugh RL, Lauber CL, Weintraub MN, et al. Stoichiometry of soil enzyme activity at global scale. Ecology Letters, 2008, 11: 1252-1264
[45] Campbell TP, Ulrich D, Toyoda JG, et al. Microbial communities influence soil dissolved organic carbon concentration by altering metabolite composition. Frontiers in Microbiology, 2022, 12: 799014
[46] Yan SB, Yin LM, Dijkstra FA, et al. Priming effect on soil carbon decomposition by root exudate surrogates: A meta-analysis. Soil Biology and Biochemistry, 2023, 178: 108955
[47] 王钰婷, 徐志伟, 孙德敬, 等. 水位恢复对排水泥炭沼泽土壤酶活性的影响. 生态学杂志, 2022, 41(10): 1940-1947
[48] 韩子琛, 郭强, 夏允, 等. 亚热带三种林分土壤酶活性和酶化学计量比特征. 应用生态学报, 2024, 35(6): 1501-1508