Abstract: Pisolithus tinctorius can form ectomycorrhizae with pine and eucalyptus.The aqueous,ethanol and acetone extracts from its fruitbodies significantly inhibited the seedling growth of Echinochloa crusgalli and Oryza sativa, and acetone extract at concentration of 0.0125g·ml^-1 inhibited the seedling growth of Pennisetum alopecuroides and Brassica campastris. Two pure compounds were isolated from the fruitbodies of P.tinctorius by silica gel column chromatography, and were identified as pisolactone and ergosterol. Ergosterol significantly inhibited the root growth of E.crusgalli seedlings at concentration of 400μg·ml^-1,and pisolactone could do it at concentration of 100μg·ml^-1.

Key words: Pisolithus tinctorius, Allelopathy, Pisolactone, Ergosterol