Abstract: The growth and physiological characters of 1～3 years old Huangshan pine seedlings in pure and mixture plots with four different transparancy were determined,and the germination percentage of seeds and their complex stressing vigour were tested.Meanwhile,the regeneration mechanism of Huangshan pine in natural forest was studied in this paper.The results showed that the plumpness of seeds was 65%,the germination percentage was 27%,and the complex stressing vigour was 26%.The lower germination percentage affected the reproduction of Huangshan pine.The photosynthetic rate was more affected by solar energy,but less by humidity and surface temperature.With high light composation,Huangshan pine's regeneration under forest was difficult.The seedlings of Huangshan pine in mixed coniferous and broadleave tree plots were at a disadvantage because of slow growth and insufficient ligth.

Key words: Pinus huangshanensis, Regeneration, Species protection, tea plant, soil microbial community, phospholipid fatty acid., microbial utilization rate of carbon source, ratooning continuous cropping