Response of some protective enzymes in Brassica chinensis seedlings to Pb2+, Cd2+ and Cr6+ stresses

Abstract

Abstract: Seedlings of Brassica chinensis were cultured in 1/2 Hoagland solution for seven days before they were treated with Pb²⁺, Cd²⁺ and Cr⁶⁺. The activities of their superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) responded to the increasing concentrations of the metals, and the responsive characters varied with different metals and different durations of metal treatments. CATactivity decreased with the increase of metal concentrations, and the order of its sensitivity to the three metals was Cd²⁺>Cr⁶⁺> Pb²⁺. SOD activity followed a rise-fall pattern along metal concentration gradient, but its sensitivity decreased with the duration of metal treatment. There was an obvious difference in PODactivity to different metals, only when the treatment was proceeded for a period of time.

Key words: Brassica chinensis, Protective enzymes, Lead, Cadmium, Chromium

CLC Number:

Q945
X171.5

REN Anzhi, GAO Yubao, LIU Shuang . Response of some protective enzymes in Brassica chinensis seedlings to Pb²⁺, Cd²⁺ and Cr⁶⁺ stresses[J]. Chinese Journal of Applied Ecology, 2002, (4): 510-512.

References

[1] Anderson MD,Prasad TK,Stewart CR.1995.Changes in isozyme profiles of cata lase,peroxidase and glutathione reductase during acclimation to chilling in mes ocotyls of maize seedlings.Plant Physiol,109 :1247～1257
[2] Cakmak I and Horst WJ.1991.Effect of aluminium on lipid peroxidation,supero xide dismutase,catalase and peroxidase activities in root tips of soybean.Physiol Plantarum,83:463～468
[3] Casano LM,Martin M,Sabater B.1994.Sensitivity of superoxide dismutase tra nscript levels and activities to oxidative stress is lower in mature-senescent than in young barley leaves.Plant Physiol,106:1033～1039
[4] Deng B-Y(邓碧玉),Yuan Q-S(袁勤生),Li W-J(李文杰).1991.Improved meth od of measuring SOD activities with 1,2,3-trihydroxy-benzene self-oxidation.Progr Biochem Biophys(生化与生物物理进展),18(2):163(in Chinese)
[5] Elstner EF.1982.Oxygen activation and oxygen toxicity.Ann Rev Pla nt Physiol,33:73～96
[6] Jiang M-Y(蒋明义),Jing J-H(荆家海),Wang S-T(王韶唐).1991.Effects of osmotic stress on membrane-lipid peroxidation and endogenous protective system s in rice seedlings.Acta Phytophysiol Sin(植物生理学报),17(1):80～84(in Chinese)
[7] Lee EH.1982.Superoxide dismutase.Plant Physiol,69:1444～1449
[8] Luo L-X(罗立新),Sun T-H(孙铁珩).1998.Effect of cadmium-surfactant co mbined pollution on physiological characteristics of wheat leaf.Chin J Appl Ecol(应用生态学报),9(1):95～1 00(in Chinese)
[9] Yan B(晏斌),Dai Q-J(戴秋杰),Liu X-Z(刘晓忠).1995.Accumulation of su peroxide radical in corn leaves during waterlogging.Acta Bot Sin(植物学报),37(9):738～744(in Chinese)
[10] Yan C-L(严重玲),Hong Y-T(洪业汤),Fu S-Z(付舜珍).1997.Effect of Cd,Pb stress on scarenging system of activated oxygen in leaves of tobacco.Acta Ecol Sin(生态学报),17(5):488～492(in Chinese)

[1]	WANG Fei, XIAO Yu, CHENG Xiao-mao, HUANG Xiao-xia. Effects of cadmium stress on growth and cadmium enrichment of Chlorophytum comosum and Chlorophytum comosum var. variegatum. [J]. Chinese Journal of Applied Ecology, 2021, 32(5): 1835-1844.
[2]	PAN Bo-gui, MO Han-qian, WANG Wei, CAI Kun-zheng, TIAN Ji-hui, CAI Yi-xia. Regulating effects of silicon on Cd-accumulation and stress-resistant responding in rice seedling [J]. Chinese Journal of Applied Ecology, 2021, 32(3): 1096-1104.
[3]	PANG Mei-xia, HUANG Zhi-li, LIU Xiao-long, TANG Yong-jun, LI Xiao-dong, JIN Gang. Effects of heavy metal cadmium on Caulerpa lentillifera based on transcriptome analysis [J]. Chinese Journal of Applied Ecology, 2021, 32(12): 4447-4456.
[4]	YOU Yue, WU Cai-nan, ZHONG Wei-jie, HOU Zhi-lin, LIU Yue, DU Shao-ting, JIN Chong-wei. Effects of dicyandiamide on cadmium accumulation in pakchoi under instant soluble nitrogen fertilizers [J]. Chinese Journal of Applied Ecology, 2020, 31(9): 3093-3100.
[5]	WANG Dun-fei, ZHENG Xin-yu, XIAO Qing-tie, WANG Wei, LIN Rui-yu. Effects of Pseudomonas aeruginosa on root activity and leaf physiological characteristics in rice (Oryza sativa L.) seedling under cadmium stress [J]. Chinese Journal of Applied Ecology, 2019, 30(8): 2767-2774.
[6]	NING Chu-han, LI Wen-bin, XU Qi-kai, LI Min, GUO Shao-xia. Arbuscular mycorrhizal fungi enhance cadmium uptake of wetland plants in contaminated water [J]. Chinese Journal of Applied Ecology, 2019, 30(6): 2063-2071.
[7]	ZHANG Jia-lei, GUO Feng, LI Xin-guo, YANG Sha, GENG Yun, MENG Jing-jing, FAN Zhong-xue, WAN Shu-bo. Effects of spraying paclobutrazol at different stages on physiological characteristics, yield and quality of peanut. [J]. Chinese Journal of Applied Ecology, 2018, 29(3): 874-882.
[8]	YUAN Man, XU Ying-chun, NIU Ye-qing, ZHOU Hui, AN Yi-lin, JIN Qi-jiang, WANG Yan-jie. Effects of ethylene and NO on AsA-GSH in lotus under cadmium stress [J]. Chinese Journal of Applied Ecology, 2018, 29(10): 3433-3440.
[9]	GUO Jing-yi, ZHANG Man, ZHANG Xi-zhou, LI Ting-xuan, ZHANG Lu, HUANG Fu, CHEN Dong-ming, SUN Xian-ming. Characterization of cadmium uptake kinetics in cadmium pollution-safe rice material. [J]. Chinese Journal of Applied Ecology, 2018, 29(1): 278-284.
[10]	ZHANG Xiao-na, PIAO Chun-lan, DONG You-kui, CUI Min-long. Transcriptome analysis of response to heavy metal Cd stress in soybean root [J]. Chinese Journal of Applied Ecology, 2017, 28(5): 1633-1641.
[11]	LI Meng, CHEN Jia-yue, XIE Jing-fang, LYU Jia-li, GAO Shao-long, LIU Rui-qing. Effects of sewage and Cd combined stress on metabolites of maize seedling [J]. Chinese Journal of Applied Ecology, 2017, 28(11): 3767-3774.
[12]	LIU Yuan, MA Wen-chao, ZHANG Wen, ZENG Cheng-cheng, CHEN Jin-ping, WEI Hong. Effect of cadmium stress on root vigor and accumulation of elements Ca, Mg, Mn, Zn, Fe in Salix variegate. [J]. Chinese Journal of Applied Ecology, 2016, 27(4): 1109-1115.
[13]	ZHANG Ran-ran, ZHANG Peng, DU Shao-ting. Oxidative stress-related signals and their regulation under Cd stress: A review [J]. Chinese Journal of Applied Ecology, 2016, 27(3): 981-992.
[14]	. Absorption and distribution of K, Na and Mg in Avicennia marina seedlings under cadmium stress. [J]. Chinese Journal of Applied Ecology, 2015, 26(5): 1313-1319.
[15]	LI He, LIAN Hai-feng, LIU Shi-qi, YU Xin-hui, SUN Ya-li, GUO Hui-ping. Effect of cadmium stress on physiological characteristics of garlic seedlings and the alleviation effects of exogenous calcium. [J]. Chinese Journal of Applied Ecology, 2015, 26(4): 1193-1198.

Response of some protective enzymes in Brassica chinensis seedlings to Pb²⁺, Cd²⁺ and Cr⁶⁺ stresses

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments