Molecular responses of rice (Oryza sativa L.) to the stress of lowly enhanced UV-B radiation

Abstract

Abstract: The approach of mRNA differential display (DD) was used to analyze the gene differential expression of rice seedlings subjected to lowly enhanced Ultraviolet Bradiation. The results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as RUVB1 and RUVB2 respectively,which could be recovered and re amplified, were specifically induced. Sequence analysis for RUVB2 through Internet BLASTsearching indicated that it was more than95% identities with three rice ESTs, but very low similarity to others in the GenBank, and was mapped on the OSJNBb0091N21BAC(GenBank accession No.:AC091122) in Oryza sativa L.

Key words: Molecular responses, mRNA differential display, Oryza sativa L., UV-B radiation

CLC Number:

S511

ZENG Jianmin, LIN Wenxiong, LIANG Kangjing, CHEN Fangyu, GUO Yuchun, TANG Lina, HE Huaqin, WANG Jingyuan, LIANG Yiyuan . Molecular responses of rice (Oryza sativa L.) to the stress of lowly enhanced UV-B radiation[J]. Chinese Journal of Applied Ecology, 2003, (6): 941-944.

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