Application of ISSR technology in genetic diversity detection of Jute

Abstract

Abstract: The genetic diversity among 27 accessions of Corchorus, including 10 Jute species, was investigated with ISSR technique.283 DNA bands were amplified with 25 ISSR primers, among which, 263 (92.85%) were polymorphic, with 10.48 bands per primer in average.Afurther systemic cluster analysis indicated that the accessions could be clustered into three groups, and the group Ⅱ (including two cultispecies and their close wild species) was obviously genetically different from the groups I and Ⅲ (including eight wild species).Moreover, 16 accessions in group Ⅱ presented a higher intraspecific genetic resemblance, while 11 accessions among groups I & Ⅲ showed an abundant interspecific genetic diversity.After synthesized the relevant findings of morphology and DNA classification, it's found that C.urticifolius could be one of the original wild species, C.tilaculariszic was a variation of C.tilaculari, and Tian Jute could be an untitled wild species.

Key words: Jute, ISSR, Genetic diversity, Consanguinity

CLC Number:

Q941

QI Jianmin, ZHOU Dongxin, WUWeiren, LIN Lihui, WU Jianmei, FANG Pingping . Application of ISSR technology in genetic diversity detection of Jute[J]. Chinese Journal of Applied Ecology, 2003, (9): 1473-1477.

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